The Application of Active Respiratory Gating Technology in Lung Cancer Radiotherapy
Issue:
Volume 2, Issue 3, June 2014
Pages:
25-29
Received:
28 August 2014
Accepted:
10 September 2014
Published:
20 September 2014
Abstract: Objective: The technology of ABC ( Active Breathing Control) was used in breath-holding time in order to improve the accuracy of radiotherapy for lung cancer patients, and the influence of respiratory movement on diaphragm motion was analyzed and evaluated, in addition the feasibility and limitation of ABC was also assessed. Methods: 15 lung cancer patients were selected for the training of ABC, at the beginning of the training, let the patients practice breath-holding by themselves at home: the practice time is 30 minutes a day, one week practice, therefore the patients slowly adapted to the new breath-holding time, which is more than 40 seconds. In the CT scan and reset simulation, the images of ABC and FB( Free Breathing) were collected respectively; At the same time of precise radiation treatment, the error values between the CT images by using ABC system and the images of the real-time scans were collected. Results: The breath-holding time of the 15 patients were more than 40 seconds, and their average breath-holding time achieved 58.3 seconds, it is longer than that of the patients before training by 24.9 seconds. The diaphragm motion is reduced by 16.76mm than that of the free breathing. In the precise radiotherapy by using ABC, the average errors of left and right, head and feet , front and back were 0.29mm, 0.51mm, 0.49mm; After the bed displacement, the average errors were 0.08cm,0.18cm,0.15 cm. Conclusions: After using ABC system in the precise radiotherapy, the average error range of left and right, head and feet , front and back was within 2mm, and the respiratory diaphragm motion was smaller than before, so the radiation effect became more accurate, and this provided more protection for the patients.
Abstract: Objective: The technology of ABC ( Active Breathing Control) was used in breath-holding time in order to improve the accuracy of radiotherapy for lung cancer patients, and the influence of respiratory movement on diaphragm motion was analyzed and evaluated, in addition the feasibility and limitation of ABC was also assessed. Methods: 15 lung cancer...
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LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
Kaszuba-Zwoińska Jolanta,
Chorobik Paulina,
Nowak Bernadeta,
Ziomber Agata,
Juszczak Kajetan,
Zaraska Wiesław,
Thor Piotr
Issue:
Volume 2, Issue 3, June 2014
Pages:
30-36
Received:
27 August 2014
Accepted:
11 September 2014
Published:
30 September 2014
Abstract: Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
Abstract: Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of ...
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