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Antidiabetic Potential of Turmeric with/without Fermented Milk Enriched with Probiotics in Diabetic Rats
Issue:
Volume 1, Issue 1, June 2013
Pages:
1-7
Received:
26 April 2013
Accepted:
Published:
30 May 2013
Abstract: Purpose: To investigate the effect of turmeric supplement (T), fermented milk with Probiotic strain Bifidobacterium lactis DN-173 010 (P), a combination of (T+P) on glycemia, lipidemia, and oxidative status in streptozotocin–induced diabetic rats.Materials and methods: Thirty male albino diabetic rats were fed a normal diet and divided to groups (n=6/group) according to the following treatments for 45 days: Turmeric (T) (225mg/kg/d), fermented milk Probiotics (P) (0.5ml/kg/d), Turmeric-Probiotics combination (T+ P) (225mg/kg/d + 0.5ml/kg/d), Oral hypoglycemic agent (OHA) (0.5 ml of glibenclamide; 450 µg/kg/day), and non-treated (DM). Blood glucose and glycayted hemoglobin were determined after diabetes induction and at the end of experiment. Serum insulin, total cholesterol (TC), LDL-C, HDL-C, TG, malondialdehyde (MDA), and total antioxidant capacity (TOAC) biomarker of oxidative stress were determined at the end of experiment. Results: All treatments resulted in a decrease in glucose and HbA1C compared to pretreatment. No difference in insulin concentration was observed. Serum TC was reduced by (T), while LDL-C decreased with (T) and (T+P) treatments. HDL-C was elevated with all treatments compared to control and (OHA) groups. MDA decreased with all treatments. TOAC was elevated with (T) and (T+P) but not with (P) treatment. Conclusion: Administration of Turmeric, Probiotics, and T+P combination were capable in attenuating hyperglycemia, dyslipidemia and oxidative stress initiated by STZ. However, (T) and (T+P) exerted a more potent hypolipidemic, and antioxidative effect compared to (P) alone.
Abstract: Purpose: To investigate the effect of turmeric supplement (T), fermented milk with Probiotic strain Bifidobacterium lactis DN-173 010 (P), a combination of (T+P) on glycemia, lipidemia, and oxidative status in streptozotocin–induced diabetic rats.Materials and methods: Thirty male albino diabetic rats were fed a normal diet and divided to groups (n...
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Estimation of Apparent Digestibility Coefficient of Plant Feed Ingredients (Soybean and Sunflower Meal) for Labeo Rohita
Farkhanda Asad,
Tooba Rehman,
Noureen Aziz Qureshi,
Nimra Tahir
Issue:
Volume 1, Issue 1, June 2013
Pages:
8-11
Received:
14 May 2013
Accepted:
Published:
10 June 2013
Abstract: The present study was carried out to estimate the apparent nutrient digestibility of soybean meal and sunflower meal for Labeo rohita. Prior to being fed with test diets, the fish were acclimatized on reference diet for about one week. Test diet-1 (soybean meal) and test diet-2 (sunflower meal) was prepared by mixing reference diet and test feed ingredients (soybean meal and sunflower meal) in a 70:30 ratio. Representative samples of feed and feces of fish were analyzed for the dry matter, crude protein, crude fat, crude fiber, ash and gross energy following AOAC (1990) to determine the apparent digestibility of soybean and sunflower meal for Labeo rohita. The outcomes showed that the apparent digestibility coefficient (ADC) of dry matter was highest (64.50%±3.5) for reference diet followed by soybean meal (34.5%±2.50) and sunflower meal (23.5%±1.50). In case of crude protein, ADC was highest for sunflower meal (77%±3.0) followed by soybean meal (63.5%±2.50) and reference diet (37.5%±0.50) while the apparent crude fat digestibility coefficient was highest for soybean meal (71.50%±3.50) followed by sunflower meal (70.50%±0.50) and reference diet (61.00%±3.0). Percentage value of apparent digestibility for crude fiber was maximum for test diet 2 (31.50%±3.50) followed by test diet 1 (11.50%±0.50) and reference diet (9.50%±0.50). In case of ash, ADC was highest for reference diet (46.50%±2.5) followed by test diet 2 (27.50%±2.5) and test diet 1 (15.50%±1.5). Values for gross energy were (43%±2.0), (42.5%±0.5) and (26.5%±7.5) for test diet 2, test diet 1 and reference diet, respectively. These findings can be sum up as soybean meal and sunflower meal proved as a promising fish feed ingredient being more efficiently utilized and digested in Labeo rohita, thus fish meal can be efficiently replaced with plant ingredients in rohu diets.
Abstract: The present study was carried out to estimate the apparent nutrient digestibility of soybean meal and sunflower meal for Labeo rohita. Prior to being fed with test diets, the fish were acclimatized on reference diet for about one week. Test diet-1 (soybean meal) and test diet-2 (sunflower meal) was prepared by mixing reference diet and test feed in...
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Binding of Heavy Metals with C-Phycocyanin: A Comparison between Equilibrium Dialysis, Fluorescence and Absorption Titration
Issue:
Volume 1, Issue 1, June 2013
Pages:
12-16
Received:
7 May 2013
Accepted:
Published:
10 June 2013
Abstract: The energetic of interaction of metal ions with C-Phycocyanin (C-PC) Spirulina platensis were investigated using optical and thermodynamic methods. Comparison of the binding constants of heavy metals with C-PC by the instrumentality of difference methods shown, that binding affinities of toxic and heavy metal ions for C-PC strongly depend upon the metal. The efficiency of interaction for different metals is arranged in the same sequence for the absorption titration as it is for fluorescence. Obtained results using equilibrium dialysis are in good agreement with results using fluorescence spectroscopy in the case Ag+, but difference is observed for Ni2+ and Cr3+ ions.
Abstract: The energetic of interaction of metal ions with C-Phycocyanin (C-PC) Spirulina platensis were investigated using optical and thermodynamic methods. Comparison of the binding constants of heavy metals with C-PC by the instrumentality of difference methods shown, that binding affinities of toxic and heavy metal ions for C-PC strongly depend upon the ...
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Biological activities of collagen peptides obtained by enzymic Hydrolysis from Far-Eastern holothurians
Popov A.,
Popov A.,
Artyukov A.,
Krivoshapko O.,
Kozlovskaya E.
Issue:
Volume 1, Issue 1, June 2013
Pages:
17-26
Received:
30 May 2013
Accepted:
Published:
30 June 2013
Abstract: The certain sea cucumbers are the marine animals which are important as human delicacy food source. In addition a number of unique biological and pharmacological active compounds have been ascribed to various species of sea cucumbers. In current work the collagen peptides (CPs) from the bodies of the far-eastern holothurians (sea cucumber) Apostichopus japonicus and Cucumaria japonica by treated with a complex of proteolytic enzymes from Kamchatka crab Paralithodes camtschaticus were obtained, and the antitumor, anticoagulant, anti-inflammatory, and wound healing properties these CPs were estimated. The element and amino acid analysis of CPs from trepang and cucumaria are suggested that it’s could be regarded as a typical collagen fragments containing 6% sulfated carbohydrate and/or amino acid residues. CPs inhibited growth and progression solid Erlich tumor, but not in alike degree. Our results clearly showed, that these CPs possessed the moderate anticoagulant activity, and are preferential inhibitors of the initial link of the blood coagulation system. We also determined that the CPs revealed significant wound healing effect in regarding to thermal wounds and have high anti-inflammatory activity by used carrageenane model of acute inflammatory. Apparently, that the degree of biological activity depend on characteristics of amino acid composition and concentration of CPs. Taken together, these results indicate that CPs from sea cucumbers have similar to biological activities with other collagen peptides (components of the extracellular matrix, endostatin and others) and sulfated polysaccharides. We proposed that the obtained CPs may be applied as components functional food and nontoxic remedies of supplementary therapy for prevention and treatment of various diseases.
Abstract: The certain sea cucumbers are the marine animals which are important as human delicacy food source. In addition a number of unique biological and pharmacological active compounds have been ascribed to various species of sea cucumbers. In current work the collagen peptides (CPs) from the bodies of the far-eastern holothurians (sea cucumber) Apostich...
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Assessment of the Prophylactic Efficacy of a Crude Aqueous Extract of Morinda Lucida Leaves on Plasmodium Falciparum Infection of Albino Rats
Oko Augustine Okpani,
Nweke Friday Nwalo,
Ugwu Okechukwu Ogbonnia,
Ehihia Loveth Ude.
Issue:
Volume 1, Issue 1, June 2013
Pages:
27-31
Received:
20 May 2013
Accepted:
Published:
30 June 2013
Abstract: A study on the prophylactic efficacy of a crude aqueous extract of the leaves of Morinda lucida on plasmodium falciparum was carried out using sixteen albino rats grouped into curative, suppressive, prophylactic and control ;A,B,C and D respectively. The rats were inoculated with the parasites and allowed for 21days before 2mls of the extract was administered to each of the rats in group A twice daily for 3days, and for group B, 2mls was administered just once, 3hours before scarifying them, while for group C, 2mls was given twice daily for 3days before inoculation with the parasites, but group D were not given the extract. The results showed that the extract cleared the parasites in group A rats and did not allow its growth in group C rats, but did not suppress it in group B rats, while the control showed the ability of the parasites surviving in the rats. There was a significant at (P< 0.05) weight loss by the rats in all the groups, following the inoculation of the parasite. It can be inferred therefore, that a crude aqueous extract of Morinda lucida has a prophylactic efficacy in addition to its curative activity against plasmodium parasites and could be used to mitigate the impact of plasmodium parasites.
Abstract: A study on the prophylactic efficacy of a crude aqueous extract of the leaves of Morinda lucida on plasmodium falciparum was carried out using sixteen albino rats grouped into curative, suppressive, prophylactic and control ;A,B,C and D respectively. The rats were inoculated with the parasites and allowed for 21days before 2mls of the extract was ...
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The Use of Enzymatic Hydrolyzate of Chlorophytum Comosum with Experimental Toxic Liver Damage in Rats
Areshidze David,
Timchenko Luydmila,
Кozlova Maria
Issue:
Volume 1, Issue 1, June 2013
Pages:
32-36
Received:
26 May 2013
Accepted:
Published:
30 July 2013
Abstract: Investigation of the influence of the enzymatic hydrolyzate of Chlorophytum comosumthe first on the liver of rats at its toxic damage showed that the substrate has expressed a pronounced hepatoprotective effect. Under its influence in the rat liver morphological changes induced by CCl4 are much less pronounced than in the controls. There are also less significant deviations from normal levels of ALT, AST and bilirubin. Information analysis of the state of the organ indicates that the level of adaptation and regeneration opportunities of the liver of rats treated with an enzymatic hydrolyzate of Chlorophytum comosum is significantly higher than that of the liver of rats with experimental toxic liver damage without the use of tested substrate.
Abstract: Investigation of the influence of the enzymatic hydrolyzate of Chlorophytum comosumthe first on the liver of rats at its toxic damage showed that the substrate has expressed a pronounced hepatoprotective effect. Under its influence in the rat liver morphological changes induced by CCl4 are much less pronounced than in the controls. There are also l...
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