Frontiers in Environmental Microbiology

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Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere

Received: Dec. 30, 2017    Accepted: Feb. 24, 2018    Published: Mar. 21, 2018
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Abstract

The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.

DOI 10.11648/j.fem.20180402.12
Published in Frontiers in Environmental Microbiology ( Volume 4, Issue 2, April 2018 )
Page(s) 45-54
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Tea, Biodiversity, Plant Growth-Promoting Rhizobacteria, Biology, Carbon Source Utilization, Acid Tolerant Strains

References
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    Atefeh Varmazyari, Ramazan Çakmakçı. (2018). Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Frontiers in Environmental Microbiology, 4(2), 45-54. https://doi.org/10.11648/j.fem.20180402.12

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    ACS Style

    Atefeh Varmazyari; Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front. Environ. Microbiol. 2018, 4(2), 45-54. doi: 10.11648/j.fem.20180402.12

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    AMA Style

    Atefeh Varmazyari, Ramazan Çakmakçı. Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere. Front Environ Microbiol. 2018;4(2):45-54. doi: 10.11648/j.fem.20180402.12

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  • @article{10.11648/j.fem.20180402.12,
      author = {Atefeh Varmazyari and Ramazan Çakmakçı},
      title = {Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere},
      journal = {Frontiers in Environmental Microbiology},
      volume = {4},
      number = {2},
      pages = {45-54},
      doi = {10.11648/j.fem.20180402.12},
      url = {https://doi.org/10.11648/j.fem.20180402.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.fem.20180402.12},
      abstract = {The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Diversity and Metabolic Potential of the Dominant Culturable N2-Fixing and P-Solubilising Bacteria from Tea (Camellia sinensis L.) Rhizosphere
    AU  - Atefeh Varmazyari
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    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
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    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20180402.12
    AB  - The purpose of this study was to investigate the diversity of cultivable N2-fixing and P-solubilizing bacteria originated from 167 rhizospheric acidic soils samples of tea. Based on the fatty acid methyl ester profiles, 34 bacterial genera were identified with a similarity index of >0.3, but 69.2% of the identified isolates belonged to five genera: Bacillus, Pseudomonas, Paenibacillus, Stenotrophomonas and Arthrobacter. Among the 263 bacterial strains, 213 strains exhibited N2-fixing activity and 159 were efficient in phosphate solubilisation; 134 strains were efficient in N2-fixation and P-solubilisation. Most of the N2-fixing and P-solubilizing bacteria isolated were Gram-positive (59.3 and 52.8%), and Gram-negative constituted only 40.7 and 47.2%. A total of 102 dominant strains were characterized by carbon sources using BIOLOGM GN2 and GP2 plates. B. pumilus, B. subtilis, B. licheniformis, B. laevolacticus, P. fluorescens, P. putida, S. maltophilia and B. megaterium were the most frequent P-solubilizing and N2-fixing species in the tea rhizosphere soils. Utilization of high variety of C-sources by the N2-fixing and P-solubilizing acid tolerant strains may play an important role in adapting to a variety of crop plants, and thus potentially beneficial to the growth of tea plants in that specific acidic ecosystem.
    VL  - 4
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Author Information
  • Department of Agronomy, Faculty of Agriculture, Atatürk University, Erzurum, Turkey

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