Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey

Postoienko Volodymyr; Bezpalyi Ivan; Postoienko Hanna

doi:doi:10.11648/j.ajaf.20221003.12

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Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey

Postoienko Volodymyr, Bezpalyi Ivan, Postoienko Hanna

Published in American Journal of Agriculture and Forestry (Volume 10, Issue 3)

Received: 23 January 2022 Accepted: 13 February 2022 Published: 19 May 2022

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Abstract

The influence of biotechnological factors, such as purebred families and honey base on the ethology of bees in the process of nectar collection and the species composition of the association of probiotic cultures in freshly pumped honey to bees was found out. The research was conducted using Ukrainian steppe bees. The following most typical external features of their breed, namely the length of the proboscis from 6.34 to 6.44 mm, cubital index in the range of 2.17 ± 0.04 - 2.61 ± 0.05, discoidal displacement 76 - 100 %, the shape of the edge of the wax mirror 68 - 96% of convex cases. The species composition of honey plants in typical conditions of the Forest-Steppe zone of Ukraine, their contribution to the general honey harvest during the whole beekeeping season and the formation of antibacterial defense mechanism of bees based on the association of probiotic cultures in their intestines and freshly pumped honey are analyzed. Data on differentiation of selected cultures from bee honey by cultural-morphological and biochemical properties are given. Probiotic cultures were identified by cultivation on differential media. Thus, cultures of Lactobacillus plantarum and Bifidobacterium bifidum were accumulated on MRS medium (solid and liquid), and Enterococcus faecium was accumulated on tryptone soy medium. It is shown that the quantitative characteristics of honey accumulation in the bee nest depend on a number of biotechnological factors. The main indicators that affect the ethology of bees when collecting nectar are purebred and species composition of the honey base. The results of the research serve as a basis for further studies of the ethology of the Ukrainian steppe breed of bees during honey collection, its popularization and use in large apiaries (100 and more families) according to the breed zoning plan in the Forest-Steppe zone of Ukraine and for the development of new antibacterial, specific for bees, organic products based on probiotic cultures, isolated from honey.

Published in	American Journal of Agriculture and Forestry (Volume 10, Issue 3)
DOI	10.11648/j.ajaf.20221003.12
Page(s)	94-99
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biotechnological Factors, Ethology of Bees, Ukrainian Steppe Breed, Honey Base, Honey Collection, Freshly Pumped Honey, Probiotic Cultures

References

[1]	Alpatov, V. V. (1948) Porody medonosnoj pchely. Moskva: Izdatel'stvo Moskovskogo obshhestva ispytatelej prirody [In Russian].
[2]	Bilash, G. D., & Krivcov, N. I. (1991). Selekcija pchel. Moskva: Agropromizdat. [In Russian].
[3]	Bodnarchuk, L. I., Bahrii, I. H., & Buhera, S. I. (1996). Pleminna robota u bdzhilnytstvi z osnovamy biometrii. Kyiv: Instytut bdzhilnytstva im. P. I. Prokopovycha UAAN. [In Ukrainian].
[4]	Bodnarchuk, L. I., Solomakha, T. D., & Illiash, A. M. (1993). Atlas medonosnykh roslyn Ukrainy. Kyiv [In Ukrainian].
[5]	Brovarskyi, V. D., Brindza, Ya., & Velychko, S. M. (2015). Etolohiia bdzhil pry formuvanni zapasiv bilkovoho kormu. Ahrobioriznomanittia Dlia Pokrashchannia Kharchuvannia, Zdorovia i Yakosti Zhyttia, 1, 65–68. [In Ukrainian].
[6]	Brovarskyi, V. D., Brindza, Ya., Otchenashko, V. V., Povoznikov, M. H., & Adamchuk, L. O., (2017). Metodyka doslidnoi spravy u bdzhilnytstvi. Kyiv: Vydavnychyi dim «Vinnichenko» [in Ukrainian].
[7]	Davydenko, I. K., Mykytenko, H. D., & Chelak, S. O. (1992). Pleminna robota u bdzhilnytstvi. Kyiv: Urozhai [In Ukrainian].
[8]	Galatiuk, A. E. (2014). The etiology and preventive maintenance of collapse of bee colonies. Bee World, 4, 1–3. [In Russian].
[9]	Hrynyk, S. (2008) Ekolohichni aspekty vyrobnytstva produktiv bdzhilnytstva. Ukrainskyi Pasichnyk, 7, 33–37. [In Ukrainian].
[10]	Lazarieva, L. M., Postoienko, V. O., & Shtanhret, L. M. (2017) Pylkovyi analiz medu z riznykh rehioniv Ukrainy. Tvarynnytstvo Ukrainy, 3–4, 26–29. [In Ukrainian].
[11]	Le Conte, Y., & Navajas, M. (2008). Influencia de los cambios climáticos en las poblaciones de abejas y sus enfermedades. Revue Scientifique et Technique de l’OIE, 27 (2), 485.
[12]	Lebedev, V. P., Irenkova, N. V., & Lebedev, V. I. (2001). Povedenie pchel pri sbore i ispolzovanii korma. Pchelovodstvo, 7, 22–24 [In Russian].
[13]	Levchenko, I. A. (2007) Skorost' obrazovanija i perestrojki pishhevyh uslovnyh refleksov u aborigennyh i zavoznyh porod pchel. Pchelovodstvo, 10, 22–25. [In Russian].
[14]	Petrenko, S. O., Petrenko, I. O., Yasko, V. M., Bohdan, M. K., Antonenko P. P., Postoienko, V. O., Reshetnichenko, O. P., Makarikhina, I. V., & Yasko, A. I. (2018). Tekhnolohiia vyrobnytstva, zberihannia ta pererobky produktsii bdzhilnytstva. Odesa: Nove vydannia [In Ukrainian].
[15]	Polishchuk, V. P. (2006). Vnutrishnoporodnyi typ ukrainskykh bdzhil «Khmelnytskyi». Pasichnyk, 1, 12–13. [In Ukrainian].
[16]	Razanov, S. F. (2008). Vyrobnytstvo medu i vosku u bahatokorpusnykh vulykakh. Tvarynnytstvo Ukrainy, 12, 43–44. [In Ukrainian].
[17]	Shatko, O. V., Dutka, L. L., & Dehodiuk, V. M. (2009). Osoblyvosti formuvannia rynku ukrainskoho medu. Pasika, 3, 2–5. [In Ukrainian].
[18]	Tsekhmistrenko, H. A. (2006). Analiz svitovoi struktury vyrobnytstva i torhivli medom. Pasika, 1, 26–29. [In Ukrainian].
[19]	Vakulenko, L. L. (2008). Teoretychni osnovy analizu rynku medu naturalnoho. Tavriiskyi Naukovyi Visnyk, 58, 231–237. [In Ukrainian].
[20]	Yatsenko, O. M. (2008). Cuchasnyi stan haluzi bdzhilnytstva u sviti ta Ukraini. Visnyk Derzhavnoho Ahroekolohichnoho Universytetu, 1, 218–226. [In Ukrainian].
[21]	«Pravyla vvezennia v Ukrainu ta vyvezennia za yii mezhi bdzhil i produktiv bdzhilnytstva»: Zakon Ukrainy No 184/82 vid 20.09.2000 r. Retrived from: https://zakon.rada.gov.ua/laws/show/z0738-00#Text [In Ukrainian].
[22]	Hosny, I. M., El-Ghani, S. Abdel, Nadir, A. S. (2009). Nutrient composition and microbiological quаlity of three unifloral honeys with emphasis on processing of honey probiotic youghurt. Global veterinaria, 3 (2). рр. 107–112 [in English].
[23]	Postoienko, V. O. Sposib profilaktyky i likuvannia infektsiinykh khvorob i stymuliatsii zhyttiediialnosti ta imunnoi systemy bdzhil iz zastosuvanniam preparativ probiotychnoi dii «Enteronormin» abo «Apinormin» [Method of prevention and treatment of infectious diseases and stimulation of life activity and immune system of bees with use of probiotic drugs «Enteronormin» or «Apinormin»]. Patent Ukrainy na korysnu model № 115447, MPK (2017.01); A 61K 35/612 (2015.01); zaiavl. 28.12.2016; opubl. 10.04.2017. Biul. № 7 [in Ukrainian].
[24]	Lazareva, L. V. (2020). Meda Ukrainy [Honey of Ukraine]. Nitra: Slovatskiy selskokhozyaystvennyy universitet v Nitre. Retrieved from http://www.slpk.sk/eldo/2020/dl/9788055222745/9788055222745.html [in Ukrainian].

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APA Style

Postoienko Volodymyr, Bezpalyi Ivan, Postoienko Hanna. (2022). Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. American Journal of Agriculture and Forestry, 10(3), 94-99. https://doi.org/10.11648/j.ajaf.20221003.12

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ACS Style

Postoienko Volodymyr; Bezpalyi Ivan; Postoienko Hanna. Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. Am. J. Agric. For. 2022, 10(3), 94-99. doi: 10.11648/j.ajaf.20221003.12

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AMA Style

Postoienko Volodymyr, Bezpalyi Ivan, Postoienko Hanna. Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. Am J Agric For. 2022;10(3):94-99. doi: 10.11648/j.ajaf.20221003.12

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@article{10.11648/j.ajaf.20221003.12,
  author = {Postoienko Volodymyr and Bezpalyi Ivan and Postoienko Hanna},
  title = {Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey},
  journal = {American Journal of Agriculture and Forestry},
  volume = {10},
  number = {3},
  pages = {94-99},
  doi = {10.11648/j.ajaf.20221003.12},
  url = {https://doi.org/10.11648/j.ajaf.20221003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20221003.12},
  abstract = {The influence of biotechnological factors, such as purebred families and honey base on the ethology of bees in the process of nectar collection and the species composition of the association of probiotic cultures in freshly pumped honey to bees was found out. The research was conducted using Ukrainian steppe bees. The following most typical external features of their breed, namely the length of the proboscis from 6.34 to 6.44 mm, cubital index in the range of 2.17 ± 0.04 - 2.61 ± 0.05, discoidal displacement 76 - 100 %, the shape of the edge of the wax mirror 68 - 96% of convex cases. The species composition of honey plants in typical conditions of the Forest-Steppe zone of Ukraine, their contribution to the general honey harvest during the whole beekeeping season and the formation of antibacterial defense mechanism of bees based on the association of probiotic cultures in their intestines and freshly pumped honey are analyzed. Data on differentiation of selected cultures from bee honey by cultural-morphological and biochemical properties are given. Probiotic cultures were identified by cultivation on differential media. Thus, cultures of Lactobacillus plantarum and Bifidobacterium bifidum were accumulated on MRS medium (solid and liquid), and Enterococcus faecium was accumulated on tryptone soy medium. It is shown that the quantitative characteristics of honey accumulation in the bee nest depend on a number of biotechnological factors. The main indicators that affect the ethology of bees when collecting nectar are purebred and species composition of the honey base. The results of the research serve as a basis for further studies of the ethology of the Ukrainian steppe breed of bees during honey collection, its popularization and use in large apiaries (100 and more families) according to the breed zoning plan in the Forest-Steppe zone of Ukraine and for the development of new antibacterial, specific for bees, organic products based on probiotic cultures, isolated from honey.},
 year = {2022}
}

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TY  - JOUR
T1  - Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey
AU  - Postoienko Volodymyr
AU  - Bezpalyi Ivan
AU  - Postoienko Hanna
Y1  - 2022/05/19
PY  - 2022
N1  - https://doi.org/10.11648/j.ajaf.20221003.12
DO  - 10.11648/j.ajaf.20221003.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 94
EP  - 99
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20221003.12
AB  - The influence of biotechnological factors, such as purebred families and honey base on the ethology of bees in the process of nectar collection and the species composition of the association of probiotic cultures in freshly pumped honey to bees was found out. The research was conducted using Ukrainian steppe bees. The following most typical external features of their breed, namely the length of the proboscis from 6.34 to 6.44 mm, cubital index in the range of 2.17 ± 0.04 - 2.61 ± 0.05, discoidal displacement 76 - 100 %, the shape of the edge of the wax mirror 68 - 96% of convex cases. The species composition of honey plants in typical conditions of the Forest-Steppe zone of Ukraine, their contribution to the general honey harvest during the whole beekeeping season and the formation of antibacterial defense mechanism of bees based on the association of probiotic cultures in their intestines and freshly pumped honey are analyzed. Data on differentiation of selected cultures from bee honey by cultural-morphological and biochemical properties are given. Probiotic cultures were identified by cultivation on differential media. Thus, cultures of Lactobacillus plantarum and Bifidobacterium bifidum were accumulated on MRS medium (solid and liquid), and Enterococcus faecium was accumulated on tryptone soy medium. It is shown that the quantitative characteristics of honey accumulation in the bee nest depend on a number of biotechnological factors. The main indicators that affect the ethology of bees when collecting nectar are purebred and species composition of the honey base. The results of the research serve as a basis for further studies of the ethology of the Ukrainian steppe breed of bees during honey collection, its popularization and use in large apiaries (100 and more families) according to the breed zoning plan in the Forest-Steppe zone of Ukraine and for the development of new antibacterial, specific for bees, organic products based on probiotic cultures, isolated from honey.
VL  - 10
IS  - 3
ER  -

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Author Information

Postoienko Volodymyr

Laboratory of Technological and Special Measures for the Prevention of Bee Diseases, Prokopovych National Beekeeping Research Institute, Kyiv, Ukraine
Bezpalyi Ivan

Faculty of Biology and Technology, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
Postoienko Hanna

Laboratory of Technological and Special Measures for the Prevention of Bee Diseases, Prokopovych National Beekeeping Research Institute, Kyiv, Ukraine

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APA Style

Postoienko Volodymyr, Bezpalyi Ivan, Postoienko Hanna. (2022). Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. American Journal of Agriculture and Forestry, 10(3), 94-99. https://doi.org/10.11648/j.ajaf.20221003.12

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ACS Style

Postoienko Volodymyr; Bezpalyi Ivan; Postoienko Hanna. Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. Am. J. Agric. For. 2022, 10(3), 94-99. doi: 10.11648/j.ajaf.20221003.12

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AMA Style

Postoienko Volodymyr, Bezpalyi Ivan, Postoienko Hanna. Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey. Am J Agric For. 2022;10(3):94-99. doi: 10.11648/j.ajaf.20221003.12

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@article{10.11648/j.ajaf.20221003.12,
  author = {Postoienko Volodymyr and Bezpalyi Ivan and Postoienko Hanna},
  title = {Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey},
  journal = {American Journal of Agriculture and Forestry},
  volume = {10},
  number = {3},
  pages = {94-99},
  doi = {10.11648/j.ajaf.20221003.12},
  url = {https://doi.org/10.11648/j.ajaf.20221003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20221003.12},
  abstract = {The influence of biotechnological factors, such as purebred families and honey base on the ethology of bees in the process of nectar collection and the species composition of the association of probiotic cultures in freshly pumped honey to bees was found out. The research was conducted using Ukrainian steppe bees. The following most typical external features of their breed, namely the length of the proboscis from 6.34 to 6.44 mm, cubital index in the range of 2.17 ± 0.04 - 2.61 ± 0.05, discoidal displacement 76 - 100 %, the shape of the edge of the wax mirror 68 - 96% of convex cases. The species composition of honey plants in typical conditions of the Forest-Steppe zone of Ukraine, their contribution to the general honey harvest during the whole beekeeping season and the formation of antibacterial defense mechanism of bees based on the association of probiotic cultures in their intestines and freshly pumped honey are analyzed. Data on differentiation of selected cultures from bee honey by cultural-morphological and biochemical properties are given. Probiotic cultures were identified by cultivation on differential media. Thus, cultures of Lactobacillus plantarum and Bifidobacterium bifidum were accumulated on MRS medium (solid and liquid), and Enterococcus faecium was accumulated on tryptone soy medium. It is shown that the quantitative characteristics of honey accumulation in the bee nest depend on a number of biotechnological factors. The main indicators that affect the ethology of bees when collecting nectar are purebred and species composition of the honey base. The results of the research serve as a basis for further studies of the ethology of the Ukrainian steppe breed of bees during honey collection, its popularization and use in large apiaries (100 and more families) according to the breed zoning plan in the Forest-Steppe zone of Ukraine and for the development of new antibacterial, specific for bees, organic products based on probiotic cultures, isolated from honey.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Biotechnological Evaluation of Bee Ethology During Honey Collection and Probiotic Properties of Honey
AU  - Postoienko Volodymyr
AU  - Bezpalyi Ivan
AU  - Postoienko Hanna
Y1  - 2022/05/19
PY  - 2022
N1  - https://doi.org/10.11648/j.ajaf.20221003.12
DO  - 10.11648/j.ajaf.20221003.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 94
EP  - 99
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20221003.12
AB  - The influence of biotechnological factors, such as purebred families and honey base on the ethology of bees in the process of nectar collection and the species composition of the association of probiotic cultures in freshly pumped honey to bees was found out. The research was conducted using Ukrainian steppe bees. The following most typical external features of their breed, namely the length of the proboscis from 6.34 to 6.44 mm, cubital index in the range of 2.17 ± 0.04 - 2.61 ± 0.05, discoidal displacement 76 - 100 %, the shape of the edge of the wax mirror 68 - 96% of convex cases. The species composition of honey plants in typical conditions of the Forest-Steppe zone of Ukraine, their contribution to the general honey harvest during the whole beekeeping season and the formation of antibacterial defense mechanism of bees based on the association of probiotic cultures in their intestines and freshly pumped honey are analyzed. Data on differentiation of selected cultures from bee honey by cultural-morphological and biochemical properties are given. Probiotic cultures were identified by cultivation on differential media. Thus, cultures of Lactobacillus plantarum and Bifidobacterium bifidum were accumulated on MRS medium (solid and liquid), and Enterococcus faecium was accumulated on tryptone soy medium. It is shown that the quantitative characteristics of honey accumulation in the bee nest depend on a number of biotechnological factors. The main indicators that affect the ethology of bees when collecting nectar are purebred and species composition of the honey base. The results of the research serve as a basis for further studies of the ethology of the Ukrainian steppe breed of bees during honey collection, its popularization and use in large apiaries (100 and more families) according to the breed zoning plan in the Forest-Steppe zone of Ukraine and for the development of new antibacterial, specific for bees, organic products based on probiotic cultures, isolated from honey.
VL  - 10
IS  - 3
ER  -

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