Simultaneous <i>in situ</i> Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip

Paul Alexandrov; Alexander Notchenko; Margaret Gradova; Oleg Gradov

doi:doi:10.11648/j.ajop.20150305.19

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Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip

Paul Alexandrov, Alexander Notchenko, Margaret Gradova, Oleg Gradov

Published in American Journal of Optics and Photonics (Volume 3, Issue 5)

Received: 21 July 2015 Accepted: 7 August 2015 Published: 7 September 2015

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Abstract

The need for simultaneous data recording from multiple channels and synchronization of the correlation data processing in patch-clamp on microelectrode arrays / chips with many data capture points corresponding to single channel ionic kinetic processes of individual cells leads to the idea that adaptive variation of the local potential registration conditions in multichannel devices without signal preprocessing in real time is impossible. Moreover, the advisability of direct registration coupling with the model realization for kinetic identification of the process during patch-clamp can be realized only in case of their synchronization. We propose here such a measurement system.

Published in	American Journal of Optics and Photonics (Volume 3, Issue 5)
DOI	10.11648/j.ajop.20150305.19
Page(s)	118-122
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

FRAP, Fluorescence Recovery Kinetics, Lab-on-a-Chip, Photobleaching, Photovoltage Clamp, Real Time Target Machine, Patch-Clamp, Ion Flux

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APA Style

Paul Alexandrov, Alexander Notchenko, Margaret Gradova, Oleg Gradov. (2015). Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. American Journal of Optics and Photonics, 3(5), 118-122. https://doi.org/10.11648/j.ajop.20150305.19

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ACS Style

Paul Alexandrov; Alexander Notchenko; Margaret Gradova; Oleg Gradov. Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. Am. J. Opt. Photonics 2015, 3(5), 118-122. doi: 10.11648/j.ajop.20150305.19

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AMA Style

Paul Alexandrov, Alexander Notchenko, Margaret Gradova, Oleg Gradov. Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. Am J Opt Photonics. 2015;3(5):118-122. doi: 10.11648/j.ajop.20150305.19

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@article{10.11648/j.ajop.20150305.19,
  author = {Paul Alexandrov and Alexander Notchenko and Margaret Gradova and Oleg Gradov},
  title = {Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip},
  journal = {American Journal of Optics and Photonics},
  volume = {3},
  number = {5},
  pages = {118-122},
  doi = {10.11648/j.ajop.20150305.19},
  url = {https://doi.org/10.11648/j.ajop.20150305.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajop.20150305.19},
  abstract = {The need for simultaneous data recording from multiple channels and synchronization of the correlation data processing in patch-clamp on microelectrode arrays / chips with many data capture points corresponding to single channel ionic kinetic processes of individual cells leads to the idea that adaptive variation of the local potential registration conditions in multichannel devices without signal preprocessing in real time is impossible. Moreover, the advisability of direct registration coupling with the model realization for kinetic identification of the process during patch-clamp can be realized only in case of their synchronization. We propose here such a measurement system.},
 year = {2015}
}

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TY  - JOUR
T1  - Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip
AU  - Paul Alexandrov
AU  - Alexander Notchenko
AU  - Margaret Gradova
AU  - Oleg Gradov
Y1  - 2015/09/07
PY  - 2015
N1  - https://doi.org/10.11648/j.ajop.20150305.19
DO  - 10.11648/j.ajop.20150305.19
T2  - American Journal of Optics and Photonics
JF  - American Journal of Optics and Photonics
JO  - American Journal of Optics and Photonics
SP  - 118
EP  - 122
PB  - Science Publishing Group
SN  - 2330-8494
UR  - https://doi.org/10.11648/j.ajop.20150305.19
AB  - The need for simultaneous data recording from multiple channels and synchronization of the correlation data processing in patch-clamp on microelectrode arrays / chips with many data capture points corresponding to single channel ionic kinetic processes of individual cells leads to the idea that adaptive variation of the local potential registration conditions in multichannel devices without signal preprocessing in real time is impossible. Moreover, the advisability of direct registration coupling with the model realization for kinetic identification of the process during patch-clamp can be realized only in case of their synchronization. We propose here such a measurement system.
VL  - 3
IS  - 5
ER  -

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Author Information

Paul Alexandrov

Talrose Institute for Energy Problems of Chemical Physics, RAS, Moscow, Russia
Alexander Notchenko

Institute for Information Transmission Problems, RAS, Kharkevich Institute, Moscow, Russia
Margaret Gradova

Semenov Institute of Chemical Physics, RAS, Moscow, Russia
Oleg Gradov

Talrose Institute for Energy Problems of Chemical Physics, RAS, Moscow, Russia

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APA Style

Paul Alexandrov, Alexander Notchenko, Margaret Gradova, Oleg Gradov. (2015). Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. American Journal of Optics and Photonics, 3(5), 118-122. https://doi.org/10.11648/j.ajop.20150305.19

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ACS Style

Paul Alexandrov; Alexander Notchenko; Margaret Gradova; Oleg Gradov. Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. Am. J. Opt. Photonics 2015, 3(5), 118-122. doi: 10.11648/j.ajop.20150305.19

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AMA Style

Paul Alexandrov, Alexander Notchenko, Margaret Gradova, Oleg Gradov. Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip. Am J Opt Photonics. 2015;3(5):118-122. doi: 10.11648/j.ajop.20150305.19

Copy | Download

@article{10.11648/j.ajop.20150305.19,
  author = {Paul Alexandrov and Alexander Notchenko and Margaret Gradova and Oleg Gradov},
  title = {Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip},
  journal = {American Journal of Optics and Photonics},
  volume = {3},
  number = {5},
  pages = {118-122},
  doi = {10.11648/j.ajop.20150305.19},
  url = {https://doi.org/10.11648/j.ajop.20150305.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajop.20150305.19},
  abstract = {The need for simultaneous data recording from multiple channels and synchronization of the correlation data processing in patch-clamp on microelectrode arrays / chips with many data capture points corresponding to single channel ionic kinetic processes of individual cells leads to the idea that adaptive variation of the local potential registration conditions in multichannel devices without signal preprocessing in real time is impossible. Moreover, the advisability of direct registration coupling with the model realization for kinetic identification of the process during patch-clamp can be realized only in case of their synchronization. We propose here such a measurement system.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Simultaneous in situ Detection of the Optical Fluorescence, Fluorescence Recovery Kinetics After Photobleaching & Membrane Ion Flux on the Electrophysiological Lab-on-a-Chip
AU  - Paul Alexandrov
AU  - Alexander Notchenko
AU  - Margaret Gradova
AU  - Oleg Gradov
Y1  - 2015/09/07
PY  - 2015
N1  - https://doi.org/10.11648/j.ajop.20150305.19
DO  - 10.11648/j.ajop.20150305.19
T2  - American Journal of Optics and Photonics
JF  - American Journal of Optics and Photonics
JO  - American Journal of Optics and Photonics
SP  - 118
EP  - 122
PB  - Science Publishing Group
SN  - 2330-8494
UR  - https://doi.org/10.11648/j.ajop.20150305.19
AB  - The need for simultaneous data recording from multiple channels and synchronization of the correlation data processing in patch-clamp on microelectrode arrays / chips with many data capture points corresponding to single channel ionic kinetic processes of individual cells leads to the idea that adaptive variation of the local potential registration conditions in multichannel devices without signal preprocessing in real time is impossible. Moreover, the advisability of direct registration coupling with the model realization for kinetic identification of the process during patch-clamp can be realized only in case of their synchronization. We propose here such a measurement system.
VL  - 3
IS  - 5
ER  -

Copy | Download