The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.
| Published in | European Journal of Biophysics (Volume 6, Issue 1) |
| DOI | 10.11648/j.ejb.20180601.13 |
| Page(s) | 17-22 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Vitamin C, Human Serum Albumin, Bovine Serum Albumin, UV-Absorption, Fluorescence Spectroscopy, Binding Constant, Binding Mode
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APA Style
Husain Alsamamra, Sawsan Abusharkh, Musa Abuteir. (2018). Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. European Journal of Biophysics, 6(1), 17-22. https://doi.org/10.11648/j.ejb.20180601.13
ACS Style
Husain Alsamamra; Sawsan Abusharkh; Musa Abuteir. Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. Eur. J. Biophys. 2018, 6(1), 17-22. doi: 10.11648/j.ejb.20180601.13
AMA Style
Husain Alsamamra, Sawsan Abusharkh, Musa Abuteir. Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C. Eur J Biophys. 2018;6(1):17-22. doi: 10.11648/j.ejb.20180601.13
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Download@article{10.11648/j.ejb.20180601.13,
author = {Husain Alsamamra and Sawsan Abusharkh and Musa Abuteir},
title = {Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C},
journal = {European Journal of Biophysics},
volume = {6},
number = {1},
pages = {17-22},
doi = {10.11648/j.ejb.20180601.13},
url = {https://doi.org/10.11648/j.ejb.20180601.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20180601.13},
abstract = {The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.},
year = {2018}
}
TY - JOUR T1 - Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C AU - Husain Alsamamra AU - Sawsan Abusharkh AU - Musa Abuteir Y1 - 2018/05/11 PY - 2018 N1 - https://doi.org/10.11648/j.ejb.20180601.13 DO - 10.11648/j.ejb.20180601.13 T2 - European Journal of Biophysics JF - European Journal of Biophysics JO - European Journal of Biophysics SP - 17 EP - 22 PB - Science Publishing Group SN - 2329-1737 UR - https://doi.org/10.11648/j.ejb.20180601.13 AB - The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching. VL - 6 IS - 1 ER -
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