Analysis of Peroxidase Activity and Total Phenol from <i>Spathoglottis plicata</i> Bl Plantlet Toward to <i>Fusarium oxysporum</i>

Endang Nurcahyani; Rochmah Agustrina; Erdi Suroso; Gardis Andari

doi:doi:10.11648/j.ijaas.20160206.11

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Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum

Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari

Published in International Journal of Applied Agricultural Sciences (Volume 2, Issue 6)

Received: 25 July 2016 Accepted: 14 September 2016 Published: 14 October 2016

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Abstract

Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.

Published in	International Journal of Applied Agricultural Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ijaas.20160206.11
Page(s)	79-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Spathoglottis plicata, Induced Resistance, Fusarium oxysporum, Peroxidase Activity, Total Phenol

References

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[3]	Bacon CW, Porter JK, Norred WP dan Leslie JF. 1996. Production of Fusaric Acid by Fusarium sp. Applied and Enviromental Microbiology. 62 (11): 4039-4043.
[4]	Bouizgarne B, Bouteau HEM, Frankart C, Reboutier D, Madiona K, Pennarun AM, Monestiez M, Trouverie J, Amiar Z, Briand J, Brault M, Rona JP, Ouhdouch Y, & Hadramu EI. 2006. Early Physiological Responses of Arabidopsis thaliana Cells to Fusaric Acid: Toxic and Signallling Effects. New Phytologist 169: 209–218.
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[8]	Landa BB, Cachinero-Diaz JM, Lemanceu P, Jimenez-Diaz RM & Alabouvette C. 2002. Effect of fusaric acid and phytoanticipans on growth of rhizobacteria and Fusarium oxysporum. Canadian Journal of Microbiology 48: 971-985.
[9]	Nurcahyani E, Sumardi I, Hadisutrisno B, & Suharyanto E. 2012. Supression of Development of Vanilla Foot Rot Disease (Fusarium oxysporum f. sp. vanillae) Throught In Vitro Fusaric Acid Selection. Journal of Tropical Plant Pests and Diseases. Acreditation of DIKTI No.110/DIKTI/Kep/2009. ISSN: 1411-7525. Vol. 12 /No. 1: 12-22.
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[12]	Ruzin, SE. 1999. Plant Microtechnique and Microscopy. Oxford University Press, New York.
[13]	Saravanan T, Bhaskaran R, and Muthusamy M. 2004. Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (cv. Rasthali) against Fusarium Wilt Disease. Plant Pathology Journal. 3: 72-80.
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[15]	Toyoda H, Hasyashi H and Yamamoto K. 1984. Selection of resistant tomato calli to fusaric acid. Ann. Phytopathol. Soc. Japan 50: 538–540.
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APA Style

Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. (2016). Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. International Journal of Applied Agricultural Sciences, 2(6), 79-82. https://doi.org/10.11648/j.ijaas.20160206.11

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ACS Style

Endang Nurcahyani; Rochmah Agustrina; Erdi Suroso; Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int. J. Appl. Agric. Sci. 2016, 2(6), 79-82. doi: 10.11648/j.ijaas.20160206.11

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AMA Style

Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int J Appl Agric Sci. 2016;2(6):79-82. doi: 10.11648/j.ijaas.20160206.11

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@article{10.11648/j.ijaas.20160206.11,
  author = {Endang Nurcahyani and Rochmah Agustrina and Erdi Suroso and Gardis Andari},
  title = {Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {2},
  number = {6},
  pages = {79-82},
  doi = {10.11648/j.ijaas.20160206.11},
  url = {https://doi.org/10.11648/j.ijaas.20160206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20160206.11},
  abstract = {Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.},
 year = {2016}
}

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TY - JOUR
T1 - Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum
AU - Endang Nurcahyani
AU - Rochmah Agustrina
AU - Erdi Suroso
AU - Gardis Andari
Y1 - 2016/10/14
PY - 2016
N1 - https://doi.org/10.11648/j.ijaas.20160206.11
DO - 10.11648/j.ijaas.20160206.11
T2 - International Journal of Applied Agricultural Sciences
JF - International Journal of Applied Agricultural Sciences
JO - International Journal of Applied Agricultural Sciences
SP - 79
EP - 82
PB - Science Publishing Group
SN - 2469-7885
UR - https://doi.org/10.11648/j.ijaas.20160206.11
AB - Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.
VL - 2
IS - 6
ER -

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Author Information

Endang Nurcahyani

Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Rochmah Agustrina

Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia
Erdi Suroso

Dept. of Agricultural Technology, Faculty of Agriculture, University of Lampung, Bandar Lampung, Indonesia
Gardis Andari

Dept. of Biology, Faculty of Mathematics and Natural of Science, University of Lampung, Bandar Lampung, Indonesia

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APA Style

Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. (2016). Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. International Journal of Applied Agricultural Sciences, 2(6), 79-82. https://doi.org/10.11648/j.ijaas.20160206.11

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ACS Style

Endang Nurcahyani; Rochmah Agustrina; Erdi Suroso; Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int. J. Appl. Agric. Sci. 2016, 2(6), 79-82. doi: 10.11648/j.ijaas.20160206.11

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AMA Style

Endang Nurcahyani, Rochmah Agustrina, Erdi Suroso, Gardis Andari. Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum. Int J Appl Agric Sci. 2016;2(6):79-82. doi: 10.11648/j.ijaas.20160206.11

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@article{10.11648/j.ijaas.20160206.11,
  author = {Endang Nurcahyani and Rochmah Agustrina and Erdi Suroso and Gardis Andari},
  title = {Analysis of Peroxidase Activity and Total Phenol from Spathoglottis plicata Bl Plantlet Toward to Fusarium oxysporum},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {2},
  number = {6},
  pages = {79-82},
  doi = {10.11648/j.ijaas.20160206.11},
  url = {https://doi.org/10.11648/j.ijaas.20160206.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20160206.11},
  abstract = {Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.},
 year = {2016}
}

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