Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre

doi:doi:10.11648/j.ijcems.20210703.11

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Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 3)

Received: 1 March 2021 Accepted: 12 May 2021 Published: 27 May 2021

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Abstract

Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 3)
DOI	10.11648/j.ijcems.20210703.11
Page(s)	55-63
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Boswellia Dalzielii, Inflammation, Anti-inflammatory, Anti-nociceptive, Phytochemicals, Mice

References

[1]	K. Ramkumar, S. Sharma, N. Vasudeva, and S. Kumar, “In vivo anti-inflammatory potential of various extracts of Sida tiagii Bhandari,” Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 2, pp. 5947-5952, 2012.
[2]	B. Calvino, B. Bann-Warth, P. Bertin, A. Binard, R. M. Grilo, A. Saraux, J. Sibilia, R. Revzs, and Vergne-Salle, « Pain, Inflammation, and the Nervous System / Immune System Interaction: An Introduction », Ed. ISBN, BSIP, Paris, pp. 9 – 13, 2007.
[3]	D. Wakefield, R. K. Kumar,” Life sciences,” Nature Publishing Group, pp. 1-6, 2001.
[4]	F. Batteux, C. Chéreau, and B. Weill, «immunopathology and inflammatory réaction», Ed. De Boeck, Paris, pp. 1-19, 2003.
[5]	P. Cailloce, ways of pain, pp. 1-5, 2009.
[6]	I. Vinuela-Fernandez, E. Jones, E. M. Welsh, and S. M. Fleetwood-Walker, “Pain mechanisms and their implication for the management of pain in farm and companion animals,” Veterinary Journal, vol. 174, no. 2, pp. 227-239, 2007.
[7]	J. Y. Jouzeau, M. Daouphars, A. Benani, and P. Netter, « Pharmacology and classification of cyclo-oxygénase inhibitors », Gastroénterologie Clinique Biologique, vol. 28, no. 3, pp. 7 - 17, 2004.
[8]	J. Ojewole, “Analgesic, anti-inflammatory and hypoglycaemic effects of Rhuschir indensis (Baker F.) [Anacardiaceae] stem bark aqueous extract in mice and rats, Journal of Ethnopharmacology, vol. 113, no. 2, pp. 338-345, 2007.
[9]	C. Sostres, C. J. Gargallo, M. T. Arroyo, A. Lanas, “Adverse effects of non-steroidal antiinflammatory drugs (NSAIDs, aspirin, and coxibs) on upper gastrointestinal tract”, Best practice and research clinical gastroenterology, vol. 24, no. 2, pp. 121-132, 2010.
[10]	A. Ouédraogo, A. Thiombiano, K. Hahn-Hadjali, and S. Guinko, «sexual Regeneration of Boswellia dalzielii Hutch, a valuable medicinal tree in Burkina Faso», Tropical woods and forests, vol. 289, no 286, pp. 41-42, 2006.
[11]	M. Arbonnier, Trees, shrubs and lianas of the drylands of West Africa. Cirad / MnhnUicn, Montpellier: CIRAD, MNHN, pages 541, 2000
[12]	J. M. Dalziel, “The useful plants of west Tropical Africa,” The Crown Agents, London, pp. 314-315, 1937.
[13]	J. M. Dalziel, “Useful Plants of West Tropical Africa”, Crown Agents for Overseas Governments and Administration, London, P. 315, 1956.
[14]	H. M. Burkill, “Useful Plants of West Tropical Africa,” Royal Botanical Gardens Kew, Vol. 1, P. 300, 1985.
[15]	J. B. Harbone “Phytochemical Methods a guide to modern technique of plants analysis”, Chapman and Hall, 2^nd ed. London, 1984.
[16]	G. E. Trease and W. C. Evans, Pharmacognosy, 13^th ed. Ballière Tindall, pp. 436-445, 1987.
[17]	C. A. Winter, E. A. Risley, and G. W. Nuss, “Carrageenan-induced oedema in hind paw of the rat as an assay for anti-inﬂammatory drugs, In” Proceedings for the Society of Experimental Biology and Medicine, vol. 111, no. 3, pp. 544–547, 1962.
[18]	F. B. C. Okoye, and P. O. Osadebe, “Studies on the mechanisms of anti-inflammatory activity of the extracts and fractions of Alchornea forbunda leaves,” Asian Pacific Journal of Tropical Medicine, vol. 2, no. 3, pp. 7-14, 2009.
[19]	F. Igbe, F. P. Ching, and A. Eromon,” Anti–inflammatory activity of aqueous fruit pulp extract of Huntera umbellate K Schum in acute and chronic inflammation,” Acta Poloniae Pharmaceutica, vol. 67, no. 1, pp. 81–85, 2010.
[20]	H. Hosseinzadeh, M. Ramezani, M. Fadishei, M. Mahmoudi, “Antinociceptive, antiinflammatory and acute toxicity effects of Zhumeria majdae extracts in mice and rats,” Phytomedicine, vol. 9, no. 2, pp. 135–141, 2002.
[21]	R. Koster, M. Anderson, and E. J. De Beer, “Acetic acid for analgesic screening,” Journal of Applied Physics, vol. 18, pp. 418- 420, 1959.
[22]	C. O. Ochieng, I. O. Ishola, S. A. Opiyo, L. A. O. Manguro, P. O. Owuor, and K. C. Wong, “Phytoecdysteroids from the Stem Bark of Vitex doniana and their Anti-Inflammatory Effects,” Planta Medica, vol. 79, no. 1, pp. 52–59, 2013.
[23]	D. L. Bars, M. Gozariu, and S. W. Cadden, “Animal models of nociception,” Pharmacological Reviews, vol. 53, no. 4, pp. 597– 652, 2001.
[24]	T. S. Mohemad, B. S. Darba, and G. Mahesh,” Analgesic, Antipyretic & Anti-inflammatory activity of dietary seasome oil in Experimental Animal Models,” Pharmacologia, vol. 2, no. 6, pp. 172 – 177, 2011.
[25]	D. Bothon, L. Abou, M. M. Atindehou, A. Kousséri, Yaou, E-E Bk, C. P. Agbangnan, L. lagnika, E. Wafo, F. avlessi, E-E Yarou, P. C. Bk, L. Agbangnan, E. Lagnika, F. Wafo, Avlessi, “Evaluation of antibacterial, free radical scavenging activities and phytochemical composition of Boswellia dalzielii Hutch extracts”, Journal of Pharmacognosy and Phytochemistry, vol. 8, no. 4, pp. 414-418, 2019.
[26]	E. M. Franzotti, C. V. Santos, H. M. Rodrigues, R. H. Mourao, M. R. Andrade, and A. R. Antoniolli, “Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva- branca), Journal of Ethnopharmacology, vol. 72, no. 2, pp. 273-277, 2000.
[27]	S. Banerjee, T. P. Sur, S. Mandal, P. C. Das, and S. Sikdar, “Assessment of the anti-inflammatory effects of Swertia chirata in acute and chronic experimental models in male albino rats,” Indian Journal of Pharmacology, vol. 32, no. 1, pp. 21-24, 2000.
[28]	M. I. Silva, M. R. N. d’Aquino, P. F. N. Teixeira, B. A. Moura, J. F. A. Do, S. D. P. De, S. M. Vasconcelos, and S. F. C. DE, “Central nervous system activity of acute administration of isopulegol in mice,” Pharmacology, Biochemistry and Behavior, vol. 88, no. 2, pp. 141-147, 2007.
[29]	Alamgeer, P. Nasreen, A. M. Uttra, H. Asif, W. Younis, U. H. Hasan, H. M. Irfan and A. Sharif, “Evaluation of anti-inflammatory and analgesic activities of aqueous methanolic extract of Ranunculus muricatus in albino mice”, Pakistan Journal of Pharmaceutical Sciences, Vol. 33, No. 3, pp. 1121-1126, 2020.
[30]	S. W. Sawadogo, R. Boly, M. Lompo, and N. Some, Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata, International Journal of Pharmacology, vol. 2, no. 4, pp. 435-438, 2006.
[31]	R. B. Birari, S. S. Jalapure, S. R. Changrani, S. L. Shid, M. V. Tote, and B. M. Habade, “Anti-inflammatory, Analgesic and Antipyretic effect of Hibiscus Rosa sinesis linn flower,” Pharmacology online, vol. 3, pp. 737-747, 2009.
[32]	A. Nicolaou, S. M. Pilkington, and L. E. Rhodes, “Ultraviolet-radiation induced skin inflammation: dissecting the role of bioactive lipids,” Chemistry and Physics of lipids, vol. 164, no. 6, pp. 535-543, 2011.
[33]	L. A. Chibli, K. C. Rodrigues, C. M. Gasparetto, N. C. Pinto, R. L. Fabri, E. Scio, M. S. Alves, G. Del-Vechio-Vieira, and O. V. Souza, “Anti-inflammatory effects of Bryophyllum pinnatum (Lam) Oken ethanol extract in acute and chronic cutaneous inflammation, Journal of Ethno pharmacology, vol. 154, no. 2, pp. 330-338, 2014.
[34]	A. Rauf, R. Khan, H. Khan, B. Ullah, and S. Pervez, “Antipyretic and antinociceptive potential of extract/fractions of Potentilla evestita and its isolated compound, acacetin,” BMC Complementary and Alternative Medecine, vol. 14, no. 1, pp. 448, 2014.
[35]	B. K. Das, M. M. Al-Amin, S. M. Russel, S. Kabir, R. Bhattacherjee, and J. M. A. Hannan, “Phytochemical Screening and Evaluation of Analgesic Activity of Oroxylum indicum, Indian Journal of Pharmaceutical Sciences, vol. 76, no. 6, pp. 571-575, 2014.
[36]	N. Muhammad, I. M. Barkatullah, H. Khan, M. Saeed, A. Z. Khan, and W. A. Kaleem, “In vivo screening of essential oils of Skimmia laureola leaves for antinociceptive and antipyretic activity, Asian Pacific Journal of Tropical Biomedicine vol. 3, no. 3, pp. 202-206, 2013.
[37]	L. A. Pini, G. Vitale, A. Ottani, M. Sandrini, “Naloxone reversible antinociception by Diclofenac sodium in the rat,” Journal of Pharmacology and Experimental Therapeutics, vol. 280, pp. 934-940, 1997.

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Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. (2021). Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. International Journal of Clinical and Experimental Medical Sciences, 7(3), 55-63. https://doi.org/10.11648/j.ijcems.20210703.11

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ACS Style

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int. J. Clin. Exp. Med. Sci. 2021, 7(3), 55-63. doi: 10.11648/j.ijcems.20210703.11

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AMA Style

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int J Clin Exp Med Sci. 2021;7(3):55-63. doi: 10.11648/j.ijcems.20210703.11

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@article{10.11648/j.ijcems.20210703.11,
  author = {Jeweldai Vedekoi and Sokeng Dongmo Selestin and Kamtchouing Pierre},
  title = {Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {3},
  pages = {55-63},
  doi = {10.11648/j.ijcems.20210703.11},
  url = {https://doi.org/10.11648/j.ijcems.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210703.11},
  abstract = {Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice
AU  - Jeweldai Vedekoi
AU  - Sokeng Dongmo Selestin
AU  - Kamtchouing Pierre
Y1  - 2021/05/27
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210703.11
DO  - 10.11648/j.ijcems.20210703.11
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 55
EP  - 63
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210703.11
AB  - Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.
VL  - 7
IS  - 3
ER  -

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Author Information

Jeweldai Vedekoi

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Sokeng Dongmo Selestin

Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Kamtchouing Pierre

Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

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Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. (2021). Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. International Journal of Clinical and Experimental Medical Sciences, 7(3), 55-63. https://doi.org/10.11648/j.ijcems.20210703.11

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ACS Style

Jeweldai Vedekoi; Sokeng Dongmo Selestin; Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int. J. Clin. Exp. Med. Sci. 2021, 7(3), 55-63. doi: 10.11648/j.ijcems.20210703.11

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AMA Style

Jeweldai Vedekoi, Sokeng Dongmo Selestin, Kamtchouing Pierre. Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice. Int J Clin Exp Med Sci. 2021;7(3):55-63. doi: 10.11648/j.ijcems.20210703.11

Copy | Download

@article{10.11648/j.ijcems.20210703.11,
  author = {Jeweldai Vedekoi and Sokeng Dongmo Selestin and Kamtchouing Pierre},
  title = {Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {3},
  pages = {55-63},
  doi = {10.11648/j.ijcems.20210703.11},
  url = {https://doi.org/10.11648/j.ijcems.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210703.11},
  abstract = {Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effect of Leaf Methanol Extract of Boswellia Dalzielii on the Experimental Inﬂammatory and Nociceptive Models in Albino Mice
AU  - Jeweldai Vedekoi
AU  - Sokeng Dongmo Selestin
AU  - Kamtchouing Pierre
Y1  - 2021/05/27
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210703.11
DO  - 10.11648/j.ijcems.20210703.11
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 55
EP  - 63
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210703.11
AB  - Inflammation is a physiological protective response by the organism to remove the injurious stimuli, microbial infections, and trauma. The prolong treatment for inflammation and pain with NSAID’s has been shown greater unwanted effects, so there is a rising scope for traditional medicines. Boswellia dalzielii leaves are used traditionally as folk remedies for the treatment of health problems caused by inflammation. The present study was an attempt to investigate the qualitative phytochemical constituents and the activity of methanol extract of Boswellia dalzielii leaves (MEBD) on the experimental inﬂammatory and nociceptive models in mice. Qualitative phytochemical constituents of the MEBD were performed using standard protocol. Anti-inflammatory activity was evaluated by studying carrageenan and egg-albumin induced paw oedema and xylene induced ear oedema animal models. Acetic acid-induced abdominal writhing, tail immersion and hot plate tests had been performed to evaluate the anti-nociceptive effect. This MEBD revealed the absence of alkaloids and saponins. The all doses (100, 200 and 400 mg) of the MEBD significantly (p < 0.001) inhibited the acute inflammatory process compared to the negative control. Highest percentage inhibition was 90.69±0.42% in carrageenan paw oedema test 100 mg/kg, 94.01±8.72 (Egg-albumin paw oedema test, 100 mg/kg) at 6 hrs, 57.43±0.03% (xylene test, 400 mg/kg). In anti-nociceptive experiments, MEBD of all doses were significantly reduced (p < 0.001) acetic acid-induced writhing (82.24±3.03%, 400 mg/kg). In addition, MEBD significantly increased the latency time (p < 0.001) in the tail flick (12.15±0.01, 400 mg/kg) at 90 min and in the hot plate test (13.37±0.10, 200 mg/kg) at 60 min compared to the control group. In conclusion, the study revealed that Boswellia dalzielii leaves, widely used in traditional medicine in North Cameroon, and affectively has antinociceptive and anti-inflammatory powers.
VL  - 7
IS  - 3
ER  -

Copy | Download