Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination

Leni Amalia; Aini Hariyani Nasution; Syafruddin Ilyas; Martina Amalia; Indra Nasution

doi:doi:10.11648/j.ijcoms.20241001.11

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Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination

Leni Amalia^*

, Aini Hariyani Nasution, Syafruddin Ilyas, Martina Amalia, Indra Nasution

Published in International Journal of Clinical Oral and Maxillofacial Surgery (Volume 10, Issue 1)

Received: 8 October 2024 Accepted: 31 October 2024 Published: 26 November 2024

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Abstract

The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cultured in DMEM. Viability tests of osteoblast cells were done using the MTT Assay method and repeated three times, and then results were measured using an Elisa reader. Viability of osteoblast cells in nHA 1,25 mg/ml (164,60 % ± 0,096), nHA 1,5 mg/ml (151,72 % ± 0,176), nHA 1,75 mg/ml (90,55 % ± 0,243), nHA 2 mg/ml (74,23 % ± 0,301) respectively. ANOVA test shows p < 0,05. IC50 value of hydroxyapatite nanoparticle from the unam snail’s shells to viability osteoblast cells is 2,23 mg/ml. Less concentration of hydroxyapatite nanoparticles tends to increase the viability of osteoblast cells. 1,75 mg/ml and below hydroxyapatite nanoparticles derived from unam snail shells are not toxic to osteoblast cells.

Published in	International Journal of Clinical Oral and Maxillofacial Surgery (Volume 10, Issue 1)
DOI	10.11648/j.ijcoms.20241001.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bone Graft, Viability Test, Hydroxyapatite, Osteoblast Cells, Unam Snail Shells

References

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APA Style

Amalia, L., Nasution, A. H., Ilyas, S., Amalia, M., Nasution, I. (2024). Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. International Journal of Clinical Oral and Maxillofacial Surgery, 10(1), 1-7. https://doi.org/10.11648/j.ijcoms.20241001.11

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ACS Style

Amalia, L.; Nasution, A. H.; Ilyas, S.; Amalia, M.; Nasution, I. Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. Int. J. Clin. Oral Maxillofac. Surg. 2024, 10(1), 1-7. doi: 10.11648/j.ijcoms.20241001.11

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AMA Style

Amalia L, Nasution AH, Ilyas S, Amalia M, Nasution I. Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. Int J Clin Oral Maxillofac Surg. 2024;10(1):1-7. doi: 10.11648/j.ijcoms.20241001.11

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@article{10.11648/j.ijcoms.20241001.11,
  author = {Leni Amalia and Aini Hariyani Nasution and Syafruddin Ilyas and Martina Amalia and Indra Nasution},
  title = {Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination
},
  journal = {International Journal of Clinical Oral and Maxillofacial Surgery},
  volume = {10},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijcoms.20241001.11},
  url = {https://doi.org/10.11648/j.ijcoms.20241001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcoms.20241001.11},
  abstract = {The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cultured in DMEM. Viability tests of osteoblast cells were done using the MTT Assay method and repeated three times, and then results were measured using an Elisa reader. Viability of osteoblast cells in nHA 1,25 mg/ml (164,60 % ± 0,096), nHA 1,5 mg/ml (151,72 % ± 0,176), nHA 1,75 mg/ml (90,55 % ± 0,243), nHA 2 mg/ml (74,23 % ± 0,301) respectively. ANOVA test shows p < 0,05. IC50 value of hydroxyapatite nanoparticle from the unam snail’s shells to viability osteoblast cells is 2,23 mg/ml. Less concentration of hydroxyapatite nanoparticles tends to increase the viability of osteoblast cells. 1,75 mg/ml and below hydroxyapatite nanoparticles derived from unam snail shells are not toxic to osteoblast cells.
},
 year = {2024}
}

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TY  - JOUR
T1  - Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination

AU  - Leni Amalia
AU  - Aini Hariyani Nasution
AU  - Syafruddin Ilyas
AU  - Martina Amalia
AU  - Indra Nasution
Y1  - 2024/11/26
PY  - 2024
N1  - https://doi.org/10.11648/j.ijcoms.20241001.11
DO  - 10.11648/j.ijcoms.20241001.11
T2  - International Journal of Clinical Oral and Maxillofacial Surgery
JF  - International Journal of Clinical Oral and Maxillofacial Surgery
JO  - International Journal of Clinical Oral and Maxillofacial Surgery
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2472-1344
UR  - https://doi.org/10.11648/j.ijcoms.20241001.11
AB  - The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cultured in DMEM. Viability tests of osteoblast cells were done using the MTT Assay method and repeated three times, and then results were measured using an Elisa reader. Viability of osteoblast cells in nHA 1,25 mg/ml (164,60 % ± 0,096), nHA 1,5 mg/ml (151,72 % ± 0,176), nHA 1,75 mg/ml (90,55 % ± 0,243), nHA 2 mg/ml (74,23 % ± 0,301) respectively. ANOVA test shows p < 0,05. IC50 value of hydroxyapatite nanoparticle from the unam snail’s shells to viability osteoblast cells is 2,23 mg/ml. Less concentration of hydroxyapatite nanoparticles tends to increase the viability of osteoblast cells. 1,75 mg/ml and below hydroxyapatite nanoparticles derived from unam snail shells are not toxic to osteoblast cells.

VL  - 10
IS  - 1
ER  -

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Author Information

Leni Amalia

Department of Periodontics, University Sumatera Utara, Medan, Indonesia

Contact Email

http://orcid.org/0009-0009-8240-6656
Aini Hariyani Nasution

Department of Periodontics, University Sumatera Utara, Medan, Indonesia
Syafruddin Ilyas

Department of Biology, University Sumatera Utara, Medan, Indonesia
Martina Amalia

Department of Periodontics, University Sumatera Utara, Medan, Indonesia
Indra Nasution

Department of Mechanical Engineering, University Sumatera Utara, Medan, Indonesia

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Plain Text BibTeX RIS

APA Style

Amalia, L., Nasution, A. H., Ilyas, S., Amalia, M., Nasution, I. (2024). Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. International Journal of Clinical Oral and Maxillofacial Surgery, 10(1), 1-7. https://doi.org/10.11648/j.ijcoms.20241001.11

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ACS Style

Amalia, L.; Nasution, A. H.; Ilyas, S.; Amalia, M.; Nasution, I. Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. Int. J. Clin. Oral Maxillofac. Surg. 2024, 10(1), 1-7. doi: 10.11648/j.ijcoms.20241001.11

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AMA Style

Amalia L, Nasution AH, Ilyas S, Amalia M, Nasution I. Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination. Int J Clin Oral Maxillofac Surg. 2024;10(1):1-7. doi: 10.11648/j.ijcoms.20241001.11

Copy | Download

@article{10.11648/j.ijcoms.20241001.11,
  author = {Leni Amalia and Aini Hariyani Nasution and Syafruddin Ilyas and Martina Amalia and Indra Nasution},
  title = {Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination
},
  journal = {International Journal of Clinical Oral and Maxillofacial Surgery},
  volume = {10},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijcoms.20241001.11},
  url = {https://doi.org/10.11648/j.ijcoms.20241001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcoms.20241001.11},
  abstract = {The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cultured in DMEM. Viability tests of osteoblast cells were done using the MTT Assay method and repeated three times, and then results were measured using an Elisa reader. Viability of osteoblast cells in nHA 1,25 mg/ml (164,60 % ± 0,096), nHA 1,5 mg/ml (151,72 % ± 0,176), nHA 1,75 mg/ml (90,55 % ± 0,243), nHA 2 mg/ml (74,23 % ± 0,301) respectively. ANOVA test shows p < 0,05. IC50 value of hydroxyapatite nanoparticle from the unam snail’s shells to viability osteoblast cells is 2,23 mg/ml. Less concentration of hydroxyapatite nanoparticles tends to increase the viability of osteoblast cells. 1,75 mg/ml and below hydroxyapatite nanoparticles derived from unam snail shells are not toxic to osteoblast cells.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination

AU  - Leni Amalia
AU  - Aini Hariyani Nasution
AU  - Syafruddin Ilyas
AU  - Martina Amalia
AU  - Indra Nasution
Y1  - 2024/11/26
PY  - 2024
N1  - https://doi.org/10.11648/j.ijcoms.20241001.11
DO  - 10.11648/j.ijcoms.20241001.11
T2  - International Journal of Clinical Oral and Maxillofacial Surgery
JF  - International Journal of Clinical Oral and Maxillofacial Surgery
JO  - International Journal of Clinical Oral and Maxillofacial Surgery
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2472-1344
UR  - https://doi.org/10.11648/j.ijcoms.20241001.11
AB  - The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cultured in DMEM. Viability tests of osteoblast cells were done using the MTT Assay method and repeated three times, and then results were measured using an Elisa reader. Viability of osteoblast cells in nHA 1,25 mg/ml (164,60 % ± 0,096), nHA 1,5 mg/ml (151,72 % ± 0,176), nHA 1,75 mg/ml (90,55 % ± 0,243), nHA 2 mg/ml (74,23 % ± 0,301) respectively. ANOVA test shows p < 0,05. IC50 value of hydroxyapatite nanoparticle from the unam snail’s shells to viability osteoblast cells is 2,23 mg/ml. Less concentration of hydroxyapatite nanoparticles tends to increase the viability of osteoblast cells. 1,75 mg/ml and below hydroxyapatite nanoparticles derived from unam snail shells are not toxic to osteoblast cells.

VL  - 10
IS  - 1
ER  -

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