Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity

Maryam A. S. Abubakr; Wedad M. Al-Adiwish

doi:doi:10.11648/j.ijmb.20170202.12

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Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity

Maryam A. S. Abubakr, Wedad M. Al-Adiwish

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 2)

Received: 28 October 2016 Accepted: 26 December 2016 Published: 20 January 2017

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Abstract

A total of eight from thirty LAB isolated from fruits samples showed clear zone on modified de Man, Rogosa and Sharp (MRS)-CaCO₃ agar, catalase negative and Gram positive were considered as LAB. From six out of eight isolates was observed good proteolytic activity when tested on skim milk agar. The isolates were identified with species of morphology and biochemical analysis using API 50 CHL kit assay and genotype identification. It can be concluded that isolated LAB from different fruits sources have probiotic properties and proteolytic properties which could benefit consumers.

Published in	International Journal of Microbiology and Biotechnology (Volume 2, Issue 2)
DOI	10.11648/j.ijmb.20170202.12
Page(s)	58-64
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Lactic Acid Bacteria, Proteolytic Activity, Probiotic Properties, Antimicrobial Activity

References

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APA Style

Maryam A. S. Abubakr, Wedad M. Al-Adiwish. (2017). Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. International Journal of Microbiology and Biotechnology, 2(2), 58-64. https://doi.org/10.11648/j.ijmb.20170202.12

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ACS Style

Maryam A. S. Abubakr; Wedad M. Al-Adiwish. Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. Int. J. Microbiol. Biotechnol. 2017, 2(2), 58-64. doi: 10.11648/j.ijmb.20170202.12

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AMA Style

Maryam A. S. Abubakr, Wedad M. Al-Adiwish. Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. Int J Microbiol Biotechnol. 2017;2(2):58-64. doi: 10.11648/j.ijmb.20170202.12

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@article{10.11648/j.ijmb.20170202.12,
  author = {Maryam A. S. Abubakr and Wedad M. Al-Adiwish},
  title = {Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {2},
  pages = {58-64},
  doi = {10.11648/j.ijmb.20170202.12},
  url = {https://doi.org/10.11648/j.ijmb.20170202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170202.12},
  abstract = {A total of eight from thirty LAB isolated from fruits samples showed clear zone on modified de Man, Rogosa and Sharp (MRS)-CaCO3 agar, catalase negative and Gram positive were considered as LAB. From six out of eight isolates was observed good proteolytic activity when tested on skim milk agar. The isolates were identified with species of morphology and biochemical analysis using API 50 CHL kit assay and genotype identification. It can be concluded that isolated LAB from different fruits sources have probiotic properties and proteolytic properties which could benefit consumers.},
 year = {2017}
}

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TY  - JOUR
T1  - Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity
AU  - Maryam A. S. Abubakr
AU  - Wedad M. Al-Adiwish
Y1  - 2017/01/20
PY  - 2017
N1  - https://doi.org/10.11648/j.ijmb.20170202.12
DO  - 10.11648/j.ijmb.20170202.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 58
EP  - 64
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20170202.12
AB  - A total of eight from thirty LAB isolated from fruits samples showed clear zone on modified de Man, Rogosa and Sharp (MRS)-CaCO3 agar, catalase negative and Gram positive were considered as LAB. From six out of eight isolates was observed good proteolytic activity when tested on skim milk agar. The isolates were identified with species of morphology and biochemical analysis using API 50 CHL kit assay and genotype identification. It can be concluded that isolated LAB from different fruits sources have probiotic properties and proteolytic properties which could benefit consumers.
VL  - 2
IS  - 2
ER  -

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Author Information

Maryam A. S. Abubakr

Department Botany, Faculty of Science, Zawia University, Zawia, Libya
Wedad M. Al-Adiwish

Department Chemistry, Faculty of Science, Zawia University, Zawia, Libya

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APA Style

Maryam A. S. Abubakr, Wedad M. Al-Adiwish. (2017). Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. International Journal of Microbiology and Biotechnology, 2(2), 58-64. https://doi.org/10.11648/j.ijmb.20170202.12

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ACS Style

Maryam A. S. Abubakr; Wedad M. Al-Adiwish. Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. Int. J. Microbiol. Biotechnol. 2017, 2(2), 58-64. doi: 10.11648/j.ijmb.20170202.12

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AMA Style

Maryam A. S. Abubakr, Wedad M. Al-Adiwish. Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity. Int J Microbiol Biotechnol. 2017;2(2):58-64. doi: 10.11648/j.ijmb.20170202.12

Copy | Download

@article{10.11648/j.ijmb.20170202.12,
  author = {Maryam A. S. Abubakr and Wedad M. Al-Adiwish},
  title = {Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {2},
  pages = {58-64},
  doi = {10.11648/j.ijmb.20170202.12},
  url = {https://doi.org/10.11648/j.ijmb.20170202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170202.12},
  abstract = {A total of eight from thirty LAB isolated from fruits samples showed clear zone on modified de Man, Rogosa and Sharp (MRS)-CaCO3 agar, catalase negative and Gram positive were considered as LAB. From six out of eight isolates was observed good proteolytic activity when tested on skim milk agar. The isolates were identified with species of morphology and biochemical analysis using API 50 CHL kit assay and genotype identification. It can be concluded that isolated LAB from different fruits sources have probiotic properties and proteolytic properties which could benefit consumers.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Identification of Lactic Acid Bacteria from Different Fruits with Proteolytic Activity
AU  - Maryam A. S. Abubakr
AU  - Wedad M. Al-Adiwish
Y1  - 2017/01/20
PY  - 2017
N1  - https://doi.org/10.11648/j.ijmb.20170202.12
DO  - 10.11648/j.ijmb.20170202.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 58
EP  - 64
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20170202.12
AB  - A total of eight from thirty LAB isolated from fruits samples showed clear zone on modified de Man, Rogosa and Sharp (MRS)-CaCO3 agar, catalase negative and Gram positive were considered as LAB. From six out of eight isolates was observed good proteolytic activity when tested on skim milk agar. The isolates were identified with species of morphology and biochemical analysis using API 50 CHL kit assay and genotype identification. It can be concluded that isolated LAB from different fruits sources have probiotic properties and proteolytic properties which could benefit consumers.
VL  - 2
IS  - 2
ER  -

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