New Simple Media Substrates to Differentiate <i>Candida dubliniensis</i> from <i>Candida albicans</i>

Zenab Abduhall Krema; Ebtesam Salem Mokthar; Hameda Sadkali El Magrahi; Mohamed Suliman Ellabib

doi:doi:10.11648/j.ijmb.20190401.14

| Peer-Reviewed

New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans

Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 1)

Received: 5 March 2019 Accepted: 8 April 2019 Published: 15 May 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.

Published in	International Journal of Microbiology and Biotechnology (Volume 4, Issue 1)
DOI	10.11648/j.ijmb.20190401.14
Page(s)	19-23
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Candida albicans, C. dubliniensis, Chlamydospore Production, Differential Medium, Seeds, Leaves

References

[1]	SULLIVAN, D. J.; WESTERNENG, T. J.; HAYNES, K. A.; BENNETT, D. E. & COLEMAN, D. C. – Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidiasis in HIV-infected individuals. Microbiology, 1995. 141: 1507-1521.
[2]	SULLIVAN, D. & COLEMAN, D. - Candida dubliniensis: characteristics and identification. J. Clin. Microbiol. 1998. 36: 329-334.
[3]	GRAF, B.; TROST, A.; EUCKER, J.; GOBEL, U. B. & ADAM, T. - Rapid and simple differentiation of C. dubliniensis from C. albicans. Diagn. Microbiol. Infect. Dis. 2004. 48: 149-151.
[4]	KURZAI, O.; HEINZ, W. J.; SULLIVAN, D. J. et al. - Rapid PCR test for discrimination between Candida albicans and Candida dubliniensis isolates using primers derived from pH-regulated PHR1 and PHR2 genes of C. albicans. J. Clin. Microbiol. 1999. 37: 1587-1590,
[5]	MOSCA, C. O.; MORAGUES, M. D.; LLOVO, J. et al. - Casein agar: a useful medium for differentiating Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 2003. 41: 1259-1262.
[6]	AL MOSAID, A.; SULLIVAN, D.; SALKIN, I. F.; SHANLEY, D. & COLEMAN, D. C. - Differentiation of Candida dubliniensis from Candida albicans on Staib agar and caffeic acid-ferric citrate agar. J. Clin. Microbiol. 2001. 39: 323-327.
[7]	ALVES, S. H.; HORTA, J. A.; MILÁN, E. P. et al. - Carbohydrate assimilation profiles of Brazilian Candida dubliniensis isolates based on ID 32C system. Rev. Inst. Med. trop. S. Paulo. 2005. 47: 109-111.
[8]	GALES, A. C.; PFALLER, M. A.; HOUSTON, A. K. et al. - Identification of Candida dubliniensis based on temperature and utilization of xylose and ∝-methyl – D-glucoside as determined with API 20C AUX and Vitek YBC systems. J. Clin. Microbiol. 1999. 37: 3804-3808.
[9]	BIKANDI, J.; MILLAN, R. S.; MORAGUES, M. D. et al. - Rapid identification of Candida dubliniensis by indirect immunofluorescence based on differential localization of antigens on C. dubliniensis blastospores and Candida albicans germ tubes. J. Clin. Microbiol. 1998. 36: 2428-2433.
[10]	STAIB, P. & MORSCHHAUSER, J. - Chlamydospore formation on Staib agar as a species-specific characteristic of Candida dubliniensis. Mycoses. 1999. 42: 521-524.
[11]	AL MOSAID, A.; SULLIVAN, D. J. & COLEMAN, D. C. - Differentiation of Candida dubliniensis from Candida albicans on Pal´s agar. J. Clin. Microbiol. 2003. 41: 4787- 4789.
[12]	KHAN, Z. U.; AHMAD, S.; MOKADDAS, E. & CHANDY, R. - Tobacco agar, a new medium for differentiating Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 2004. 42: 4796-4798.
[13]	KIRKPATRICK, W. R.; REVANKAR, S. G.; McATEE, R. K. et al. - Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates. J. Clin. Microbiol. 1998. 36: 3007-3012.
[14]	PINJON, E.; SULLIVAN, D.; SALKIN, I.; SHANLEY, D. & COLEMAN, D. - Simple, inexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 1998. 36: 2093-2095.
[15]	ALVES, S. H.; MILAN, E. P.; SANT’ANA, P. L. et al. - Hypertonic Sabouraud broth as a simple and powerful test for Candida dubliniensis screening. Diagn. Microbiol. Infect. Dis. 2002. 43: 85-86.
[16]	PELTROCHE-LLACSAHUANGA, H.; SCHMIDT, S.; SEIBOLD, M.; LUTTICKEN, R. & HAASE, G. - Differentiation between Candida dubliniensis and Candida albicans by fatty acid methyl ester analysis using gas-liquid chromatography. J. clin. Microbiol. 2000. 38: 3696-3704.
[17]	SELVARANGAN, R.; LIMAYE, A. P. & COOKSON, B. T. - Rapid identification and differentiation of Candida albicans and Candida dubliniensis by capillary-based amplification and fluorescent probe hybridization. J. clin. Microbiol. 2002. 40: 4308- 4312.
[18]	Krema ZA, Trfas EEM, Ellabib MS, Cogliati M2. Oral carriage of candidiasis in patients with oral dental diseases: predisposing factors, species and their antifungal susceptibility patterns. Journal of Bacteriology & Mycology: Open Access. 2018, 6(3):1-5.
[19]	Mohamed H, Krema Z, Mokthar E, Ellabib M*, El Magrahi H and andEshwika A. Oral Candida colonization in HIV infected patients: Species and antifungal susceptibility in Tripoli/Libya. International Journal of Clinical Microbiology and Biochemical Technology: Open Access. 2018. (3): 1-8
[20]	LARONE, D. H. - Medically important fungi: a guide to identification. 3. ed. Washington, ASM Press, 1995.
[21]	Gutierrez J, Morales P, Gonzalez MA, Quindos G. Candida dubliniensis, a new fungal pathogen. J Basic Microbiol. 2002; 3:207-27.
[22]	Khan ZU, Ahmad S, Mokaddas E, Chandy R. Simplified sunflower (Helianthus annuus) seed agar for differentiation of Candida dubliniensis from Candida albicans. Clin Microbiol Infect 2004. 10:590-2.
[23]	Ulia P, Clarissa R, Michael F and Markus R. Comparison of Phenotypic Methods for the Identification of Candida dubliniensis. J Microbiol Immunol Infect. 2010. 43(2):147–154.
[24]	Mohamed H, Krema Z, Mokthar E, Ellabib M*, El Magrahi H and andEshwika A and Cogliati M. Evaluation of novel culture media prepared from plant substrates for isolation and identification of Cryptococcus Neoformans Species Complex. International Journal of Clinical Microbiology and Biochemical Technology: Open Access. 14. August, 2018. (1): 1-8
[25]	Sydney HA, Érico Silva de L, Carlos EL, Carolina P. S, Liliane A. S etal. Comparison among tomato juice agar with other three media for differentiation of Candida dubliniensis from candida albicans. Rev. Inst. Med. trop. S. Paulo. 2006. 48(3):119-121.

Cite This Article

Plain Text BibTeX RIS

APA Style

Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. (2019). New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. International Journal of Microbiology and Biotechnology, 4(1), 19-23. https://doi.org/10.11648/j.ijmb.20190401.14

Copy | Download

ACS Style

Zenab Abduhall Krema; Ebtesam Salem Mokthar; Hameda Sadkali El Magrahi; Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int. J. Microbiol. Biotechnol. 2019, 4(1), 19-23. doi: 10.11648/j.ijmb.20190401.14

Copy | Download

AMA Style

Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int J Microbiol Biotechnol. 2019;4(1):19-23. doi: 10.11648/j.ijmb.20190401.14

Copy | Download

@article{10.11648/j.ijmb.20190401.14,
  author = {Zenab Abduhall Krema and Ebtesam Salem Mokthar and Hameda Sadkali El Magrahi and Mohamed Suliman Ellabib},
  title = {New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {4},
  number = {1},
  pages = {19-23},
  doi = {10.11648/j.ijmb.20190401.14},
  url = {https://doi.org/10.11648/j.ijmb.20190401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190401.14},
  abstract = {Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.},
 year = {2019}
}

Copy | Download

TY - JOUR
T1 - New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans
AU - Zenab Abduhall Krema
AU - Ebtesam Salem Mokthar
AU - Hameda Sadkali El Magrahi
AU - Mohamed Suliman Ellabib
Y1 - 2019/05/15
PY - 2019
N1 - https://doi.org/10.11648/j.ijmb.20190401.14
DO - 10.11648/j.ijmb.20190401.14
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 19
EP - 23
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20190401.14
AB - Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.
VL - 4
IS - 1
ER -

Copy | Download

Author Information

Zenab Abduhall Krema

Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya
Ebtesam Salem Mokthar

Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya
Hameda Sadkali El Magrahi

Department of Medical Laboratory Science, Faculty of Medical Technology, Tripoli, Libya
Mohamed Suliman Ellabib

Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, Tripoli, Libya

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. (2019). New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. International Journal of Microbiology and Biotechnology, 4(1), 19-23. https://doi.org/10.11648/j.ijmb.20190401.14

Copy | Download

ACS Style

Zenab Abduhall Krema; Ebtesam Salem Mokthar; Hameda Sadkali El Magrahi; Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int. J. Microbiol. Biotechnol. 2019, 4(1), 19-23. doi: 10.11648/j.ijmb.20190401.14

Copy | Download

AMA Style

Zenab Abduhall Krema, Ebtesam Salem Mokthar, Hameda Sadkali El Magrahi, Mohamed Suliman Ellabib. New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans. Int J Microbiol Biotechnol. 2019;4(1):19-23. doi: 10.11648/j.ijmb.20190401.14

Copy | Download

@article{10.11648/j.ijmb.20190401.14,
  author = {Zenab Abduhall Krema and Ebtesam Salem Mokthar and Hameda Sadkali El Magrahi and Mohamed Suliman Ellabib},
  title = {New Simple Media Substrates to Differentiate Candida dubliniensis from Candida albicans},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {4},
  number = {1},
  pages = {19-23},
  doi = {10.11648/j.ijmb.20190401.14},
  url = {https://doi.org/10.11648/j.ijmb.20190401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190401.14},
  abstract = {Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.},
 year = {2019}
}

Copy | Download