Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State

Ringim Mahi Saidu; Gumel Ahmad Mohammed; Shiaka Gimba Peter

doi:doi:10.11648/j.ijmb.20200501.15

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Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State

Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 1)

Received: 26 January 2020 Accepted: 12 February 2020 Published: 19 February 2020

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Abstract

The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.

Published in	International Journal of Microbiology and Biotechnology (Volume 5, Issue 1)
DOI	10.11648/j.ijmb.20200501.15
Page(s)	28-33
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Bacteria, Bioremediation, Hydrocarbon, Oil Spill, Petroleum, Pollution, Polymerase Chain Reaction (PCR)

References

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APA Style

Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. (2020). Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. International Journal of Microbiology and Biotechnology, 5(1), 28-33. https://doi.org/10.11648/j.ijmb.20200501.15

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ACS Style

Ringim Mahi Saidu; Gumel Ahmad Mohammed; Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int. J. Microbiol. Biotechnol. 2020, 5(1), 28-33. doi: 10.11648/j.ijmb.20200501.15

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AMA Style

Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int J Microbiol Biotechnol. 2020;5(1):28-33. doi: 10.11648/j.ijmb.20200501.15

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@article{10.11648/j.ijmb.20200501.15,
  author = {Ringim Mahi Saidu and Gumel Ahmad Mohammed and Shiaka Gimba Peter},
  title = {Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {1},
  pages = {28-33},
  doi = {10.11648/j.ijmb.20200501.15},
  url = {https://doi.org/10.11648/j.ijmb.20200501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.15},
  abstract = {The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.},
 year = {2020}
}

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TY  - JOUR
T1  - Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State
AU  - Ringim Mahi Saidu
AU  - Gumel Ahmad Mohammed
AU  - Shiaka Gimba Peter
Y1  - 2020/02/19
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200501.15
DO  - 10.11648/j.ijmb.20200501.15
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200501.15
AB  - The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.
VL  - 5
IS  - 1
ER  -

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Author Information

Ringim Mahi Saidu

Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria
Gumel Ahmad Mohammed

Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria
Shiaka Gimba Peter

Department of Microbiology and Biotechnology, Faculty of science, Federal University Dutse, Dutse City, Jigawa State, Nigeria

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APA Style

Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. (2020). Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. International Journal of Microbiology and Biotechnology, 5(1), 28-33. https://doi.org/10.11648/j.ijmb.20200501.15

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ACS Style

Ringim Mahi Saidu; Gumel Ahmad Mohammed; Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int. J. Microbiol. Biotechnol. 2020, 5(1), 28-33. doi: 10.11648/j.ijmb.20200501.15

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AMA Style

Ringim Mahi Saidu, Gumel Ahmad Mohammed, Shiaka Gimba Peter. Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State. Int J Microbiol Biotechnol. 2020;5(1):28-33. doi: 10.11648/j.ijmb.20200501.15

Copy | Download

@article{10.11648/j.ijmb.20200501.15,
  author = {Ringim Mahi Saidu and Gumel Ahmad Mohammed and Shiaka Gimba Peter},
  title = {Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {1},
  pages = {28-33},
  doi = {10.11648/j.ijmb.20200501.15},
  url = {https://doi.org/10.11648/j.ijmb.20200501.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.15},
  abstract = {The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.},
 year = {2020}
}

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TY  - JOUR
T1  - Isolation and Molecular Identification of Hydrocarbon Degrading Bacteria from Contaminated Soil in Mechanic Village Dutse, Jigawa State
AU  - Ringim Mahi Saidu
AU  - Gumel Ahmad Mohammed
AU  - Shiaka Gimba Peter
Y1  - 2020/02/19
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200501.15
DO  - 10.11648/j.ijmb.20200501.15
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200501.15
AB  - The spillage of oil and other petroleum products have been a major source of environmental pollution globally. Rampant use of chemical remediation methods were shown to be noxious with detrimental consequences. Exploration of microbiodata for probable candidate to be utilized for degradation of oil spillage or contamination is of great interest. In this study, soil samples from 10 different mechanic workshops in mechanic village Dutse were collected as well as control sample from unpolluted hydrocarbon free soil at same site, bacterial species were isolated, identified using biochemical and molecular 16S rRNA gene sequencing and post sequencing analyses. Species enumerated were Bacillus cereaus, Bacillus subtilis, Bacillus licheniformis and Paenibacillus alvei, The sequences were compared to closest relative species in the GenBank data base of National Centre for Biotechnology Information. However, the substrate used was engine oil and all the isolates were found to utilized the test substrate. Biodegradation studies have been carried out through Gas Chromatography Mass Spectrometry (GC-MS). Of all the isolates, Bacillus subtilis might be the most hydrocarbon degrading bacterial species due to high average relative abundance concentration of degraded hydrocarbon compound.
VL  - 5
IS  - 1
ER  -

Copy | Download