An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Zuhair Abdul Al-Ameer Khammas; Noora Saad Mubdir

doi:doi:10.11648/j.sjac.20140205.11

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An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Zuhair Abdul Al-Ameer Khammas, Noora Saad Mubdir

Published in Science Journal of Analytical Chemistry (Volume 2, Issue 5)

Received: 13 September 2014 Accepted: 24 September 2014 Published: 30 September 2014

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Abstract

A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.

Published in	Science Journal of Analytical Chemistry (Volume 2, Issue 5)
DOI	10.11648/j.sjac.20140205.11
Page(s)	47-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Ciprofloxacin HCl, Ferric Ion, Cloud Pint Extraction, Spectrophotometry

References

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Zuhair Abdul Al-Ameer Khammas, Noora Saad Mubdir. (2014). An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Science Journal of Analytical Chemistry, 2(5), 47-54. https://doi.org/10.11648/j.sjac.20140205.11

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ACS Style

Zuhair Abdul Al-Ameer Khammas; Noora Saad Mubdir. An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Sci. J. Anal. Chem. 2014, 2(5), 47-54. doi: 10.11648/j.sjac.20140205.11

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AMA Style

Zuhair Abdul Al-Ameer Khammas, Noora Saad Mubdir. An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Sci J Anal Chem. 2014;2(5):47-54. doi: 10.11648/j.sjac.20140205.11

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@article{10.11648/j.sjac.20140205.11,
  author = {Zuhair Abdul Al-Ameer Khammas and Noora Saad Mubdir},
  title = {An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals},
  journal = {Science Journal of Analytical Chemistry},
  volume = {2},
  number = {5},
  pages = {47-54},
  doi = {10.11648/j.sjac.20140205.11},
  url = {https://doi.org/10.11648/j.sjac.20140205.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20140205.11},
  abstract = {A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.},
 year = {2014}
}

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TY  - JOUR
T1  - An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals
AU  - Zuhair Abdul Al-Ameer Khammas
AU  - Noora Saad Mubdir
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.sjac.20140205.11
DO  - 10.11648/j.sjac.20140205.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 47
EP  - 54
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20140205.11
AB  - A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.
VL  - 2
IS  - 5
ER  -

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Author Information

Zuhair Abdul Al-Ameer Khammas

Department of Chemistry, College of Science for Women, University of Baghdad, Jadiyriah, Baghdad, Iraq
Noora Saad Mubdir

Department of Chemistry, College of Science for Women, University of Baghdad, Jadiyriah, Baghdad, Iraq

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APA Style

Zuhair Abdul Al-Ameer Khammas, Noora Saad Mubdir. (2014). An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Science Journal of Analytical Chemistry, 2(5), 47-54. https://doi.org/10.11648/j.sjac.20140205.11

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ACS Style

Zuhair Abdul Al-Ameer Khammas; Noora Saad Mubdir. An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Sci. J. Anal. Chem. 2014, 2(5), 47-54. doi: 10.11648/j.sjac.20140205.11

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AMA Style

Zuhair Abdul Al-Ameer Khammas, Noora Saad Mubdir. An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals. Sci J Anal Chem. 2014;2(5):47-54. doi: 10.11648/j.sjac.20140205.11

Copy | Download

@article{10.11648/j.sjac.20140205.11,
  author = {Zuhair Abdul Al-Ameer Khammas and Noora Saad Mubdir},
  title = {An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals},
  journal = {Science Journal of Analytical Chemistry},
  volume = {2},
  number = {5},
  pages = {47-54},
  doi = {10.11648/j.sjac.20140205.11},
  url = {https://doi.org/10.11648/j.sjac.20140205.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20140205.11},
  abstract = {A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals
AU  - Zuhair Abdul Al-Ameer Khammas
AU  - Noora Saad Mubdir
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.sjac.20140205.11
DO  - 10.11648/j.sjac.20140205.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 47
EP  - 54
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20140205.11
AB  - A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) –CIPRO complex in presence of dilute H2SO4, followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL® infusion and Ciprodar® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer’ companies.
VL  - 2
IS  - 5
ER  -

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