Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

Tesfa Bedassa; Negussie Megersa; Abera Gure

doi:doi:10.11648/j.sjac.20170503.11

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Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

Tesfa Bedassa, Negussie Megersa, Abera Gure

Published in Science Journal of Analytical Chemistry (Volume 5, Issue 3)

Received: 1 May 2017 Accepted: 8 May 2017 Published: 4 July 2017

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Abstract

A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r²) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5–12.8 μg L^-1, respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.

Published in	Science Journal of Analytical Chemistry (Volume 5, Issue 3)
DOI	10.11648/j.sjac.20170503.11
Page(s)	38-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Salting-Out Liquid–Liquid Extraction, Multiclass Pesticides, Alcoholic Beverages, High Performance Liquid Chromatography, Ultraviolet-Visible Detector

References

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Tesfa Bedassa, Negussie Megersa, Abera Gure. (2017). Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Science Journal of Analytical Chemistry, 5(3), 38-45. https://doi.org/10.11648/j.sjac.20170503.11

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ACS Style

Tesfa Bedassa; Negussie Megersa; Abera Gure. Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Sci. J. Anal. Chem. 2017, 5(3), 38-45. doi: 10.11648/j.sjac.20170503.11

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AMA Style

Tesfa Bedassa, Negussie Megersa, Abera Gure. Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Sci J Anal Chem. 2017;5(3):38-45. doi: 10.11648/j.sjac.20170503.11

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@article{10.11648/j.sjac.20170503.11,
  author = {Tesfa Bedassa and Negussie Megersa and Abera Gure},
  title = {Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography},
  journal = {Science Journal of Analytical Chemistry},
  volume = {5},
  number = {3},
  pages = {38-45},
  doi = {10.11648/j.sjac.20170503.11},
  url = {https://doi.org/10.11648/j.sjac.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20170503.11},
  abstract = {A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r2) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5–12.8 μg L-1, respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.},
 year = {2017}
}

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TY  - JOUR
T1  - Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography
AU  - Tesfa Bedassa
AU  - Negussie Megersa
AU  - Abera Gure
Y1  - 2017/07/04
PY  - 2017
N1  - https://doi.org/10.11648/j.sjac.20170503.11
DO  - 10.11648/j.sjac.20170503.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 38
EP  - 45
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20170503.11
AB  - A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r2) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5–12.8 μg L-1, respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.
VL  - 5
IS  - 3
ER  -

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Author Information

Tesfa Bedassa

Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
Negussie Megersa

Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
Abera Gure

Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia

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Tesfa Bedassa, Negussie Megersa, Abera Gure. (2017). Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Science Journal of Analytical Chemistry, 5(3), 38-45. https://doi.org/10.11648/j.sjac.20170503.11

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ACS Style

Tesfa Bedassa; Negussie Megersa; Abera Gure. Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Sci. J. Anal. Chem. 2017, 5(3), 38-45. doi: 10.11648/j.sjac.20170503.11

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AMA Style

Tesfa Bedassa, Negussie Megersa, Abera Gure. Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography. Sci J Anal Chem. 2017;5(3):38-45. doi: 10.11648/j.sjac.20170503.11

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@article{10.11648/j.sjac.20170503.11,
  author = {Tesfa Bedassa and Negussie Megersa and Abera Gure},
  title = {Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography},
  journal = {Science Journal of Analytical Chemistry},
  volume = {5},
  number = {3},
  pages = {38-45},
  doi = {10.11648/j.sjac.20170503.11},
  url = {https://doi.org/10.11648/j.sjac.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20170503.11},
  abstract = {A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r2) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5–12.8 μg L-1, respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.},
 year = {2017}
}

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TY  - JOUR
T1  - Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography
AU  - Tesfa Bedassa
AU  - Negussie Megersa
AU  - Abera Gure
Y1  - 2017/07/04
PY  - 2017
N1  - https://doi.org/10.11648/j.sjac.20170503.11
DO  - 10.11648/j.sjac.20170503.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 38
EP  - 45
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20170503.11
AB  - A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r2) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5–12.8 μg L-1, respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.
VL  - 5
IS  - 3
ER  -

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