Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats

Etoundi Omgba Cunégonde Blanche; Kayo Tayou Cynthia Valère; Mbang Mbarga Audrey Judith; Piéme Constant Anatole

doi:doi:10.11648/j.wjfst.20190301.12

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Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats

Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole

Published in World Journal of Food Science and Technology (Volume 3, Issue 1)

Received: 13 February 2019 Accepted: 20 March 2019 Published: 15 May 2019

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Abstract

Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.

Published in	World Journal of Food Science and Technology (Volume 3, Issue 1)
DOI	10.11648/j.wjfst.20190301.12
Page(s)	6-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Oxidative Stress, Acute Toxicity, Lead Acetate, Edible Mushrooms, Pleurotus spp

References

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Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole. (2019). Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World Journal of Food Science and Technology, 3(1), 6-13. https://doi.org/10.11648/j.wjfst.20190301.12

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ACS Style

Etoundi Omgba Cunégonde Blanche; Kayo Tayou Cynthia Valère; Mbang Mbarga Audrey Judith; Piéme Constant Anatole. Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World J. Food Sci. Technol. 2019, 3(1), 6-13. doi: 10.11648/j.wjfst.20190301.12

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AMA Style

Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole. Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World J Food Sci Technol. 2019;3(1):6-13. doi: 10.11648/j.wjfst.20190301.12

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@article{10.11648/j.wjfst.20190301.12,
  author = {Etoundi Omgba Cunégonde Blanche and Kayo Tayou Cynthia Valère and Mbang Mbarga Audrey Judith and Piéme Constant Anatole},
  title = {Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats},
  journal = {World Journal of Food Science and Technology},
  volume = {3},
  number = {1},
  pages = {6-13},
  doi = {10.11648/j.wjfst.20190301.12},
  url = {https://doi.org/10.11648/j.wjfst.20190301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20190301.12},
  abstract = {Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.},
 year = {2019}
}

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TY  - JOUR
T1  - Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats
AU  - Etoundi Omgba Cunégonde Blanche
AU  - Kayo Tayou Cynthia Valère
AU  - Mbang Mbarga Audrey Judith
AU  - Piéme Constant Anatole
Y1  - 2019/05/15
PY  - 2019
N1  - https://doi.org/10.11648/j.wjfst.20190301.12
DO  - 10.11648/j.wjfst.20190301.12
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 6
EP  - 13
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20190301.12
AB  - Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.
VL  - 3
IS  - 1
ER  -

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Author Information

Etoundi Omgba Cunégonde Blanche

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Kayo Tayou Cynthia Valère

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Mbang Mbarga Audrey Judith

Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Piéme Constant Anatole

Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biochemical Sciences, University of Yaounde I, Yaounde, Cameroon

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Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole. (2019). Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World Journal of Food Science and Technology, 3(1), 6-13. https://doi.org/10.11648/j.wjfst.20190301.12

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ACS Style

Etoundi Omgba Cunégonde Blanche; Kayo Tayou Cynthia Valère; Mbang Mbarga Audrey Judith; Piéme Constant Anatole. Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World J. Food Sci. Technol. 2019, 3(1), 6-13. doi: 10.11648/j.wjfst.20190301.12

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AMA Style

Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole. Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats. World J Food Sci Technol. 2019;3(1):6-13. doi: 10.11648/j.wjfst.20190301.12

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@article{10.11648/j.wjfst.20190301.12,
  author = {Etoundi Omgba Cunégonde Blanche and Kayo Tayou Cynthia Valère and Mbang Mbarga Audrey Judith and Piéme Constant Anatole},
  title = {Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats},
  journal = {World Journal of Food Science and Technology},
  volume = {3},
  number = {1},
  pages = {6-13},
  doi = {10.11648/j.wjfst.20190301.12},
  url = {https://doi.org/10.11648/j.wjfst.20190301.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20190301.12},
  abstract = {Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats
AU  - Etoundi Omgba Cunégonde Blanche
AU  - Kayo Tayou Cynthia Valère
AU  - Mbang Mbarga Audrey Judith
AU  - Piéme Constant Anatole
Y1  - 2019/05/15
PY  - 2019
N1  - https://doi.org/10.11648/j.wjfst.20190301.12
DO  - 10.11648/j.wjfst.20190301.12
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 6
EP  - 13
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20190301.12
AB  - Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.
VL  - 3
IS  - 1
ER  -

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