Antioxidant Phlorotannin from Brown Algae <i>Sargassum dupplicatum</i>: Enzyme-assissted Extraction and Purification

Vu Ngoc Boi; Nguyen Thi My Trang; Dang Xuan Cuong; Hoang Thai Ha

doi:doi:10.11648/j.wjfst.20200402.17

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Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification

Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha

Published in World Journal of Food Science and Technology (Volume 4, Issue 2)

Received: 8 April 2020 Accepted: 26 April 2020 Published: 19 May 2020

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Abstract

The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and ¹H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO₄ equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO₄ equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and ¹H NMR spectrum showed phloroglucinol exist in two over fractions.

Published in	World Journal of Food Science and Technology (Volume 4, Issue 2) This article belongs to the Special Issue Marine Bio-Polymer: Bio-Activity, Extraction and Application
DOI	10.11648/j.wjfst.20200402.17
Page(s)	62-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Antioxidant, Brown Algae, Enzyme, Extraction, Lipoxygenase, Phlorotannin, Purification

References

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[3]	Vu, N. B., Dang, X. C. and Phan, T. K. V. (2017). Effects of extraction conditions over the phlorotannin content and antioxidant activity of extract from brown algae Sargassum serratum (Nguyen Huu Dai 2004). Free Radicals and Antioxidants, 7 (1): 115-22.
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Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha. (2020). Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World Journal of Food Science and Technology, 4(2), 62-68. https://doi.org/10.11648/j.wjfst.20200402.17

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ACS Style

Vu Ngoc Boi; Nguyen Thi My Trang; Dang Xuan Cuong; Hoang Thai Ha. Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World J. Food Sci. Technol. 2020, 4(2), 62-68. doi: 10.11648/j.wjfst.20200402.17

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AMA Style

Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha. Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World J Food Sci Technol. 2020;4(2):62-68. doi: 10.11648/j.wjfst.20200402.17

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@article{10.11648/j.wjfst.20200402.17,
  author = {Vu Ngoc Boi and Nguyen Thi My Trang and Dang Xuan Cuong and Hoang Thai Ha},
  title = {Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification},
  journal = {World Journal of Food Science and Technology},
  volume = {4},
  number = {2},
  pages = {62-68},
  doi = {10.11648/j.wjfst.20200402.17},
  url = {https://doi.org/10.11648/j.wjfst.20200402.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20200402.17},
  abstract = {The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.},
 year = {2020}
}

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TY  - JOUR
T1  - Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification
AU  - Vu Ngoc Boi
AU  - Nguyen Thi My Trang
AU  - Dang Xuan Cuong
AU  - Hoang Thai Ha
Y1  - 2020/05/19
PY  - 2020
N1  - https://doi.org/10.11648/j.wjfst.20200402.17
DO  - 10.11648/j.wjfst.20200402.17
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 62
EP  - 68
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20200402.17
AB  - The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.
VL  - 4
IS  - 2
ER  -

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Author Information

Vu Ngoc Boi

Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
Nguyen Thi My Trang

Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
Dang Xuan Cuong

Organic Matterial from Marine Resource, Nhatrang Institute of Technology Application and Research, Vietnam Academy of Science and Technology, Nha Trang, Vietnam
Hoang Thai Ha

Faculty of Fisheries, Ho Chi Minh University of Food Industry, Ho Chi Minh, Vietnam

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Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha. (2020). Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World Journal of Food Science and Technology, 4(2), 62-68. https://doi.org/10.11648/j.wjfst.20200402.17

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Vu Ngoc Boi; Nguyen Thi My Trang; Dang Xuan Cuong; Hoang Thai Ha. Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World J. Food Sci. Technol. 2020, 4(2), 62-68. doi: 10.11648/j.wjfst.20200402.17

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AMA Style

Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha. Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification. World J Food Sci Technol. 2020;4(2):62-68. doi: 10.11648/j.wjfst.20200402.17

Copy | Download

@article{10.11648/j.wjfst.20200402.17,
  author = {Vu Ngoc Boi and Nguyen Thi My Trang and Dang Xuan Cuong and Hoang Thai Ha},
  title = {Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification},
  journal = {World Journal of Food Science and Technology},
  volume = {4},
  number = {2},
  pages = {62-68},
  doi = {10.11648/j.wjfst.20200402.17},
  url = {https://doi.org/10.11648/j.wjfst.20200402.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20200402.17},
  abstract = {The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.},
 year = {2020}
}

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TY  - JOUR
T1  - Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification
AU  - Vu Ngoc Boi
AU  - Nguyen Thi My Trang
AU  - Dang Xuan Cuong
AU  - Hoang Thai Ha
Y1  - 2020/05/19
PY  - 2020
N1  - https://doi.org/10.11648/j.wjfst.20200402.17
DO  - 10.11648/j.wjfst.20200402.17
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 62
EP  - 68
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20200402.17
AB  - The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.
VL  - 4
IS  - 2
ER  -

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