Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review

Misgana Banti

doi:doi:10.11648/j.wjfst.20210503.11

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Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review

Misgana Banti

Published in World Journal of Food Science and Technology (Volume 5, Issue 3)

Received: 2 July 2021 Accepted: 16 July 2021 Published: 23 July 2021

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Abstract

Raffinose family oligosaccharides are important components in various plant products which 8have numbers of contributions to plant physiology and are non-digestible in monogastric 9animals and in human upper gut. Thus, they are passed into the lower gut without being digested where they are fermented and liberate a substantial amount of gas (CO₂, H₂ and CH₄) which result in flatulence and hence can be considered as anti-nutritional factors as they cause abdominal discomfort, diarrhea, flatulence and others. RFOs are widely distributed in various crops. Leguminous plants for example are one of the most important plant food materials, for they are the concentrated and low cost sources of protein for the vegan population and low income societies. However, they are under-utilized in various countries specially developed countries because of the anti-nutritional factors including flatulence factor, raffinose family oligosaccharides. Apart from these anti-nutritional properties, RFOs is also among the important food components when consumed moderately. It is among the health promoting components of foods as it facilitates the growth of important microflora in the gut, enhance the activities of antioxidant enzyme system, hepatoprotective and other multiple health benefits. Therefore, it is very important to reduce RFOs to certain optimal amount only to reduce their adverse anti-nutritional effect and utilize their advantages.

Published in	World Journal of Food Science and Technology (Volume 5, Issue 3)
DOI	10.11648/j.wjfst.20210503.11
Page(s)	37-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Anti-nutritional, Flatulence, Inhibitors, Microflora, RFOs

References

[1]	Moreno, F. J. and Sanz, M. L. eds., 2014. Food oligosaccharides: production, analysis and bioactivity. John Wiley & Sons.
[2]	Bansleben, D., Schellenberg, I. and Wolff, A. C., 2008. Highly automated and fast determination of raffinose family oligosaccharides in Lupinus seeds using pressurized liquid extraction and high-performance anion-exchange chromatography with pulsed amperometric detection. Journal of the Science of Food and Agriculture, 88 (11), pp. 1949-1953.
[3]	Singh, N. and Kayastha, A. M., 2013. A novel application of Cicer α-galactosidase in reduction of raffinose family oligosaccharides in soybean flour. Journal of Plant Biochemistry and Biotechnology, 22 (3), pp. 353-356.
[4]	Englyst, K. N., Liu, S. and Englyst, H. N., 2007. Nutritional characterization and measurement of dietary carbohydrates. European journal of clinical nutrition, 61 (1), pp. S19-S39.
[5]	Kannan, U., Sharma, R., Gangola, M. P. And Chibbar, R. N., 2018. Improving grain quality in pulses: s trategies to reduce raffinose family oligosaccharides in seeds. Ekin Journal of Crop Breeding and Genetics, 4 (1), pp. 70-88.
[6]	Álvarez-Cao, M. E., Cerdán, M. E., González-Siso, M. I. and Becerra, M., 2019. Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides. Microbial cell factories, 18 (1), pp. 1-17.
[7]	Peterbauer, T. and Richter, A., 2001. Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research, 11 (3), pp. 185-198.
[8]	Sengupta, S., Mukherjee, S., Basak, P. and Majumder, A. L., 2015. Significance of galactinol and raffinose family oligosaccharide synthesis in plants. Frontiers in plant science, 6, p. 656.
[9]	Belorkar, A. S. and Gupta, K. A., 2016. Oligosaccharides: a boom from nature’s desk. AMB Expr. 6: 8.
[10]	Tosh, S. M. and Yada, S., 2010. Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications. Food Research International, 43 (2), pp. 450-460.
[11]	Johnson, C. R., Combs Jr, G. F. and Thavarajah, P., 2013. Lentil (Lens culinaris L.): A prebiotic-rich whole food legume. Food Research International, 51 (1), pp. 107-113.
[12]	Kumar, V., Rani, A., Goyal, L., Dixit, A. K., Manjaya, J. G., Dev, J. and Swamy, M., 2010. Sucrose and raffinose family oligosaccharides (RFOs) in soybean seeds as influenced by genotype and growing location. Journal of agricultural and food chemistry, 58 (8), pp. 5081-5085.
[13]	Gustavsson, S. and Jacobsson, S., 2020. Fermentation and germination of pearl millet and cowpea.
[14]	Karnpanit, W., Coorey, R., Clements, J., Nasar-Abbas, S. M., Khan, M. K. and Jayasena, V., 2016. Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.). International Journal of Food Science & Technology, 51 (6), pp. 1386-1392.
[15]	Ekvall, J., Stegmark, R. and Nyman, M., 2007. Optimization of extraction methods for determination of the raffinose family oligosaccharides in leguminous vine peas (Pisum sativum L.) and effects of blanching. Journal of Food Composition and Analysis, 20 (1), pp. 13-18.
[16]	Mahmood, T., Hameed, T., Hasnain, S., Ali, S., Qayyum, A., Mehmood, A., Liaquat, M., Khan, S. U., Saeed, M. and Khan, A., 2017. Assessment of flatulence causing agents in Chickpea (Cicer arietinum L.) and their possible removal. Food Science and Technology, 38, pp. 120-125.
[17]	Shimelis, A. E., 2005. Influence of processing on antinutrients, raffinose family oligosaccharides and invitro protein digestibility of improved dry bean (Phaseolus vulgaris L.) varieties grown in Ethiopia (Doctoral dissertation, Asian Institute of Technology. Bangkok, Thailand).
[18]	Landry, E. J., Fuchs, S. J. and Hu, J., 2016. Carbohydrate composition of mature and immature faba bean seeds. Journal of Food Composition and Analysis, 50, pp. 55-60.
[19]	Aguilera, Y., Martín-Cabrejas, M. A., Benítez, V., Mollá, E., López-Andréu, F. J. and Esteban, R. M., 2009. Changes in carbohydrate fraction during dehydration process of common legumes. Journal of Food Composition and Analysis, 22 (7-8), pp. 678-683.
[20]	McPhee, K. E., Zemetra, R. S., Brown, J. and Myers, J. R., 2002. Genetic analysis of the raffinose family oligosaccharides in common bean. Journal of the American Society for Horticultural Science, 127 (3), pp. 376-382.
[21]	Zhang, R., Zhao, Y., Sun, Y., Lu, X. and Yang, X., 2013. Isolation, characterization, and hepatoprotective effects of the raffinose family oligosaccharides from Rehmannia glutinosa Libosch. Journal of agricultural and food chemistry, 61 (32), pp. 7786-7793.
[22]	Martı́nez-Villaluenga, C., Frı́as, J. and Vidal-Valverde, C., 2005. Raffinose family oligosaccharides and sucrose contents in 13 Spanish lupin cultivars. Food chemistry, 91 (4), pp. 645-649.
[23]	Gangola, M. P., Jaiswal, S., Khedikar, Y. P. and Chibbar, R. N., 2014. A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC–PAD and its comparison with HPLC–RI. Food chemistry, 154, pp. 127-133.
[24]	Muzquiz, M., Burbano, C., Pedrosa, M. M., Folkman, W. and Gulewicz, K., 1999. Lupins as a potential source of raffinose family oligosaccharides: Preparative method for their isolation and purification. Industrial Crops and Products, 9 (3), pp. 183-188.
[25]	Ye, F., Geng, X. R., Xu, L. J., Chang, M. C., Feng, C. P. and Meng, J. L., 2018. Purification and characterization of a novel protease-resistant GH27 α-galactosidase from Hericium erinaceus. International journal of biological macromolecules, 120, pp. 2165-2174.
[26]	Odunfa, S. A., 1983. Carbohydrate changes in fermenting locust bean (Parkia filicoidea) duringiru preparation. Plant Foods for Human Nutrition, 32 (1), pp. 3-10.
[27]	Vinjamoori, D. V., Byrum, J. R., Hayes, T. and Das, P. K., 2004. Challenges and opportunities in the analysis of raffinose oligosaccharides, pentosans, phytate, and glucosinolates. Journal of animal science, 82 (1), pp. 319-328.
[28]	Gratzfeld-Hüsgen, A. and Schuster, R., 2001. HPLC for food analysis: the fundamentals of an alternative approach to solving tomorrows measurement challenges: a primer. Hewlett-Packard.
[29]	Qiu, D., Vuong, T., Valliyodan, B., Shi, H., Guo, B., Shannon, J. G. and Nguyen, H. T., 2015. Identification and characterization of a stachyose synthase gene controlling reduced stachyose content in soybean. Theoretical and Applied Genetics, 128 (11), pp. 2167-2176.
[30]	Siddiq, M., Nyombaire, G., Dolan, K. D., Matella, N. J. and Harte, J. B., 2006. Processing of sugar-coated red kidney beans (Phaseolus vulgaris): Fate of oligosaccharides and phytohemagglutinin (PHA), and evaluation of sensory quality. Journal of food science, 71 (9), pp. C521-C526.
[31]	Carabetta, S., Di Sanzo, R., Campone, L., Fuda, S., Rastrelli, L. and Russo, M., 2020. High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC–PAD) and Chemometrics for Geographical and Floral Authentication of Honeys from Southern Italy (Calabria region). Foods, 9 (11), p. 1625.
[32]	Kelkar, S., Siddiq, M., Harte, J. B., Dolan, K. D., Nyombaire, G. and Suniaga, H., 2012. Use of low-temperature extrusion for reducing phytohemagglutinin activity (PHA) and oligosaccharides in beans (Phaseolus vulgaris L.) cv. Navy and Pinto. Food Chemistry, 133 (4), pp. 1636-1639.
[33]	Somiari, R. I. and Balogh, E., 1993. Effect of soaking, cooking and crude α-galactosidase treatment on the oligosaccharide content of cowpea flours. Journal of the Science of Food and Agriculture, 61 (3), pp. 339-343.
[34]	Carlsson, N. G., Karlsson, H. and Sandberg, A. S., 1992. Determination of oligosaccharides in foods, diets, and intestinal contents by high-temperature gas chromatography and gas chromatography/mass spectrometry. Journal of agricultural and food chemistry, 40 (12), pp. 2404-2412.
[35]	Grob, K., 1980. Gas chromatographic stationary phases analysed by capillary gas chromatography. Journal of Chromatography A, 198 (2), pp. 176-179.
[36]	Marsol-Vall, A., Balcells, M., Eras, J. and Canela-Garayoa, R., 2016. Injection-port derivatization coupled to GC–MS/MS for the analysis of glycosylated and non-glycosylated polyphenols in fruit samples. Food chemistry, 204, pp. 210-217
[37]	Kubincova, J., VIŠŇOVSKÝ, J., Rosenberg, M., Hronska, H., CHOCHULOVÁ, A., Kubinec, R. and BLAŠKO, J., 2016. Application of high-temperature GC-MS for simultaneous identification and quantification of glycosidic forms of iridoids and flavonoids in plant samples. Journal of Food & Nutrition Research, 55 (4).
[38]	Siva, N., Thavarajah, P., Kumar, S. and Thavarajah, D., 2019. Variability in prebiotic carbohydrates in different market classes of chickpea, common bean, and lentil collected from the American local market. Frontiers in nutrition, 6, p. 38.
[39]	Souframanien, J., Roja, G. and Gopalakrishna, T., 2014. Genetic variation in raffinose family oligosaccharides and sucrose content in black gram [Vigna mungo L. (Hepper)]. J Food Legumes, 27, pp. 37-41.
[40]	Dixit, A. K., Kumar, V., Rani, A., Manjaya, J. G. and Bhatnagar, D., 2011. Effect of gamma irradiation on lipoxygenases, trypsin inhibitor, raffinose family oligosaccharides and nutritional factors of different seed coat colored soybean (Glycine max L.). Radiation Physics and Chemistry, 80 (4), pp. 597-603.
[41]	Raman, M., Saiprasad, G. V. S. and Madhavakrishna, K., 2019. From seed to feed: assessment and alleviation of Raffinose Family Oligosaccharides (RFOs) of seed-and sprout-flours of soybean [Glycine max (L.) Merr.]-a commercial aspect. International Food Research Journal, 26 (1).
[42]	Barampama, Z. and Simard, R. E., 1993. Nutrient composition, protein quality and antinutritional factors of some varieties of dry beans (Phaseolus vulgaris) grown in Burundi. Food Chemistry, 47 (2), pp. 159-167.

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Misgana Banti. (2021). Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World Journal of Food Science and Technology, 5(3), 37-44. https://doi.org/10.11648/j.wjfst.20210503.11

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ACS Style

Misgana Banti. Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World J. Food Sci. Technol. 2021, 5(3), 37-44. doi: 10.11648/j.wjfst.20210503.11

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AMA Style

Misgana Banti. Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World J Food Sci Technol. 2021;5(3):37-44. doi: 10.11648/j.wjfst.20210503.11

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@article{10.11648/j.wjfst.20210503.11,
  author = {Misgana Banti},
  title = {Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review},
  journal = {World Journal of Food Science and Technology},
  volume = {5},
  number = {3},
  pages = {37-44},
  doi = {10.11648/j.wjfst.20210503.11},
  url = {https://doi.org/10.11648/j.wjfst.20210503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20210503.11},
  abstract = {Raffinose family oligosaccharides are important components in various plant products which 8have numbers of contributions to plant physiology and are non-digestible in monogastric 9animals and in human upper gut. Thus, they are passed into the lower gut without being digested where they are fermented and liberate a substantial amount of gas (CO2, H2 and CH4) which result in flatulence and hence can be considered as anti-nutritional factors as they cause abdominal discomfort, diarrhea, flatulence and others. RFOs are widely distributed in various crops. Leguminous plants for example are one of the most important plant food materials, for they are the concentrated and low cost sources of protein for the vegan population and low income societies. However, they are under-utilized in various countries specially developed countries because of the anti-nutritional factors including flatulence factor, raffinose family oligosaccharides. Apart from these anti-nutritional properties, RFOs is also among the important food components when consumed moderately. It is among the health promoting components of foods as it facilitates the growth of important microflora in the gut, enhance the activities of antioxidant enzyme system, hepatoprotective and other multiple health benefits. Therefore, it is very important to reduce RFOs to certain optimal amount only to reduce their adverse anti-nutritional effect and utilize their advantages.},
 year = {2021}
}

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TY  - JOUR
T1  - Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review
AU  - Misgana Banti
Y1  - 2021/07/23
PY  - 2021
N1  - https://doi.org/10.11648/j.wjfst.20210503.11
DO  - 10.11648/j.wjfst.20210503.11
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20210503.11
AB  - Raffinose family oligosaccharides are important components in various plant products which 8have numbers of contributions to plant physiology and are non-digestible in monogastric 9animals and in human upper gut. Thus, they are passed into the lower gut without being digested where they are fermented and liberate a substantial amount of gas (CO2, H2 and CH4) which result in flatulence and hence can be considered as anti-nutritional factors as they cause abdominal discomfort, diarrhea, flatulence and others. RFOs are widely distributed in various crops. Leguminous plants for example are one of the most important plant food materials, for they are the concentrated and low cost sources of protein for the vegan population and low income societies. However, they are under-utilized in various countries specially developed countries because of the anti-nutritional factors including flatulence factor, raffinose family oligosaccharides. Apart from these anti-nutritional properties, RFOs is also among the important food components when consumed moderately. It is among the health promoting components of foods as it facilitates the growth of important microflora in the gut, enhance the activities of antioxidant enzyme system, hepatoprotective and other multiple health benefits. Therefore, it is very important to reduce RFOs to certain optimal amount only to reduce their adverse anti-nutritional effect and utilize their advantages.
VL  - 5
IS  - 3
ER  -

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Misgana Banti

Food Science and Nutrition Research, Kulumsa Agricultural Research Center, Assella, Ethiopia

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Misgana Banti. (2021). Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World Journal of Food Science and Technology, 5(3), 37-44. https://doi.org/10.11648/j.wjfst.20210503.11

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ACS Style

Misgana Banti. Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World J. Food Sci. Technol. 2021, 5(3), 37-44. doi: 10.11648/j.wjfst.20210503.11

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AMA Style

Misgana Banti. Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review. World J Food Sci Technol. 2021;5(3):37-44. doi: 10.11648/j.wjfst.20210503.11

Copy | Download

@article{10.11648/j.wjfst.20210503.11,
  author = {Misgana Banti},
  title = {Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review},
  journal = {World Journal of Food Science and Technology},
  volume = {5},
  number = {3},
  pages = {37-44},
  doi = {10.11648/j.wjfst.20210503.11},
  url = {https://doi.org/10.11648/j.wjfst.20210503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20210503.11},
  abstract = {Raffinose family oligosaccharides are important components in various plant products which 8have numbers of contributions to plant physiology and are non-digestible in monogastric 9animals and in human upper gut. Thus, they are passed into the lower gut without being digested where they are fermented and liberate a substantial amount of gas (CO2, H2 and CH4) which result in flatulence and hence can be considered as anti-nutritional factors as they cause abdominal discomfort, diarrhea, flatulence and others. RFOs are widely distributed in various crops. Leguminous plants for example are one of the most important plant food materials, for they are the concentrated and low cost sources of protein for the vegan population and low income societies. However, they are under-utilized in various countries specially developed countries because of the anti-nutritional factors including flatulence factor, raffinose family oligosaccharides. Apart from these anti-nutritional properties, RFOs is also among the important food components when consumed moderately. It is among the health promoting components of foods as it facilitates the growth of important microflora in the gut, enhance the activities of antioxidant enzyme system, hepatoprotective and other multiple health benefits. Therefore, it is very important to reduce RFOs to certain optimal amount only to reduce their adverse anti-nutritional effect and utilize their advantages.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Raffinose Family Oligosaccharides, Occurrence in Food Materials, Nutritional Implication and Methods of Analysis, a Review
AU  - Misgana Banti
Y1  - 2021/07/23
PY  - 2021
N1  - https://doi.org/10.11648/j.wjfst.20210503.11
DO  - 10.11648/j.wjfst.20210503.11
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20210503.11
AB  - Raffinose family oligosaccharides are important components in various plant products which 8have numbers of contributions to plant physiology and are non-digestible in monogastric 9animals and in human upper gut. Thus, they are passed into the lower gut without being digested where they are fermented and liberate a substantial amount of gas (CO2, H2 and CH4) which result in flatulence and hence can be considered as anti-nutritional factors as they cause abdominal discomfort, diarrhea, flatulence and others. RFOs are widely distributed in various crops. Leguminous plants for example are one of the most important plant food materials, for they are the concentrated and low cost sources of protein for the vegan population and low income societies. However, they are under-utilized in various countries specially developed countries because of the anti-nutritional factors including flatulence factor, raffinose family oligosaccharides. Apart from these anti-nutritional properties, RFOs is also among the important food components when consumed moderately. It is among the health promoting components of foods as it facilitates the growth of important microflora in the gut, enhance the activities of antioxidant enzyme system, hepatoprotective and other multiple health benefits. Therefore, it is very important to reduce RFOs to certain optimal amount only to reduce their adverse anti-nutritional effect and utilize their advantages.
VL  - 5
IS  - 3
ER  -

Copy | Download