Pyrin Gene Polymorphisms and H. pylori-associated Dyspepsia: A Sri Lankan Study
Yashodha Weerasinghe,
Chinthika Gunasekara,
Manjula Weerasekera,
Surangi Jayakody,
Bimalka Seneviratne,
Deepaka Weerasekara,
Chaturika Jayasinghe,
Navoda Perera,
Thilini Gamage,
Neluka Fernando
Issue:
Volume 6, Issue 1, January 2021
Pages:
1-7
Received:
20 December 2020
Accepted:
11 January 2021
Published:
22 January 2021
Abstract: Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in response to pathogen infection. This study focused on the role of pyrin gene polymorphism in gastric mucosal severity and H. pylori infection. Materials and Methods: Among the ninety dyspeptic patients three gastric biopsies were taken and the presence of H. pylori, yeast species and the gastric mucosal severity was determined. EDTA blood was used for DNA extraction and identification of pyrin gene polymorphism. 12 MEFV gene mutations were tested. Results: Most of the patients (61%) had mild chronic gastritis. Among them 11.1% specimens gave positive bands for NL1/LS2 PCR of yeast DNA. H. pylori was positive in 17 patients. No homozygous mutations were found in the MEFV gene. The most common three heterozygous mutations were E148Q (45%), P369S (5%), M680I (11.6%). No significant difference was observed between the presence of the gene polymorphism, gastric mucosal severity or the presence of H. pylori and yeast species in the study group. Conclusion: The absence of homozygous mutations in the MEFV gene suggests that it is not a main factor contributing to gastric mucosal severity. The presence of H. pylori and yeasts reinforce the concept that stomach is a non-sterile environment.
Abstract: Background/Aim: A considerable high number of dyspeptic patients were reported even with the decreasing prevalence of H. pylori in Sri Lanka. Several microbial, host, and environmental factors may associate with the disease outcome. Pyrin secreted by the white blood cells may modulate the inflammatory process by assembling inflammasome complexes in...
Show More
Assessment of Biochemical and Hematological Parameters in the CRF of Before and After Hemodialysis at Martyr Mohammed El-Najjar Hospital in Gaza Strip, Palestine
Mohamad Alhamss,
Ahmad Tabash,
Abdrabbou Elregeb,
Ayman Abu Mustafa,
Asmaa Hejazi,
Riyad Shaban,
Ramy Sarafandi,
Yaser Elarja,
Nedal Hasanain,
Shawgy Elhessy,
Faten Allyan
Issue:
Volume 6, Issue 1, January 2021
Pages:
8-16
Received:
24 November 2020
Accepted:
29 December 2020
Published:
12 March 2021
Abstract: There are limited data on the assessment biochemical and hematological profile in the CRF (CRF) of before and after hemodialysis in the Gaza Strip. The study aimed to evaluating some biochemical parameters and hematological profile in the CRF of before and after hemodialysis at Martyr Mohammed El-Najjar Hospital in Rafah, Gaza Strip, Palestine. Forty-three (end-stage renal disease) ESRD patients, aged 20-70 years old who were referred to Martyr Mohammed Youssef Al-Najjar Hospital in Rafah for the hemodialysis department. 43 apparently healthy individuals matched for ages were used as a control group for comparisons. The results showed that after-dialysis higher statistically significant in Hb, RBC, HCT, PLT, AST, ALT, ALP and Ca level than before-dialysis. While Ph, WBC, RDW, creatinine (Cr.), Urea was lower statistically significant in after hemodialysis than before-dialysis (P < 0.05). theses indicate to: chronic kidney disease shows abnormal hematological parameters, precisely reduced levels of RBC count, hemoglobin, hematocrit, and platelet count, in addition, before-dialysis patients are more anemic and thrombocytopenic than after-dialysis patients and this is may indicate of lack of blood pressure and type2 diabetes mellites (T2DM) control in these patients. AST& ALT activities vary in concentration before and after hemodialysis and make the after-dialysis patients are higher than before-dialysis.
Abstract: There are limited data on the assessment biochemical and hematological profile in the CRF (CRF) of before and after hemodialysis in the Gaza Strip. The study aimed to evaluating some biochemical parameters and hematological profile in the CRF of before and after hemodialysis at Martyr Mohammed El-Najjar Hospital in Rafah, Gaza Strip, Palestine. For...
Show More
