Research Article
Investigation of the Interaction Between Ovalbumin and Gallic Acid by Spectroscopic Method
Su-Chol Rim,
Hang-Ryol Maeng*
,
Song-Jin Pak,
Yong-Su Pak,
Hyok-Won Kim
Issue:
Volume 11, Issue 1, March 2025
Pages:
1-7
Received:
10 November 2024
Accepted:
12 December 2024
Published:
21 January 2025
DOI:
10.11648/j.jddmc.20251101.11
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Abstract: Gallic acid, one of the polyphenolic compounds, has known to have many pharmaceutical and biological properties such as anti-oxidant, anti-inflammatory, anti-virus and anti-tumor activities. The interaction study of gallic acid and protein is very important in drug design, development of drug delivery system as well as immunology research. Among various techniques available for interaction study between polyphenols and small molecule, spectroscopic method is mainly used to assess protein-polyphenol complex due to its simplicity, rapidity and sensitivity. In this study, the interaction between gallic acid and ovalbumin was investigated by fluorescence quenching, synchronous and 3D (three-dimensional) fluorescence spectra. The possibility of quenching of ovalbumin by gallic acid was studied by Stern-Volmer equation. The quenching mechanism, binding constant, the number of binding sites were also investigated. The binding mode was determined using the Van’t Hoff and Gibbs-Helmholtz equation. The average distance between gallic acid and ovalbumin was calculated by Förster’s theory. The results showed that gallic acid could quench ovalbumin through static quenching mechanism. In the binding process, electrostatic forces were mainly involved. In addition, synchronous and 3D fluorescence spectroscopic analysis revealed that the microenvironments of tryptophan and tyrosine residues in ovalbumin were changed during the complex formation. The result of present study provides the information of binding mechanism of gallic acid with ovalbumin.
Abstract: Gallic acid, one of the polyphenolic compounds, has known to have many pharmaceutical and biological properties such as anti-oxidant, anti-inflammatory, anti-virus and anti-tumor activities. The interaction study of gallic acid and protein is very important in drug design, development of drug delivery system as well as immunology research. Among va...
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Research Article
Isolation and Characterization of Geranylated Chalcone from Ethylacetate Fraction of Terminalia brownii Fresen (Combretaceae)
Issue:
Volume 11, Issue 1, March 2025
Pages:
8-12
Received:
25 December 2024
Accepted:
2 February 2025
Published:
28 February 2025
Abstract: Terminalia brownii is a deciduous tree characterized by spirally arranged leaves concentrated at the ends of its branches. The plant is widely distributed in Northern Nigeria and other part of Africa, including Congo, Kenya, Sudan, Ethiopia, and Tanzania. Traditionally, it is used for treating various conditions such as gastric ulcers, epilepsy, colitis, jaundice, fungal infections, diarrhea, malaria, hepatitis, and allergic reactions. This study aimed to analyzed the crude methanol leave extract and ethyl acetate fraction of Terminalia brownii to isolate secondary metabolites. Plant material was collected, air-dried, pulverized, and extracted using cold maceration with 70% methanol. The resulting crude methanol extract was fractionated with n-hexane, chloroform, ethyl acetate, and n-butanol to yield their respective fractions. The ethyl acetate underwent extensive column chromatography using silica gel and Sephadex LH-20. Structural characterization of the isolated compound was conducted through physical and chemical analysis, UV and IR spectroscopy, advanced 1D and 2D NMR techniques, supported by literature references. This process resulted in the isolation of a yellow amorphous compound, identified as 2’,6’,4-trihydroxy-3’-methoxy-4-O-prenyloxy chalcone (geranylated chalcone). The compound is isolated for the first time from Terminalia brownii leave, this discovery convey Terminalia brownii as a rich source of secondary metabolites and contributes to the taxonomy of the plant.
Abstract: Terminalia brownii is a deciduous tree characterized by spirally arranged leaves concentrated at the ends of its branches. The plant is widely distributed in Northern Nigeria and other part of Africa, including Congo, Kenya, Sudan, Ethiopia, and Tanzania. Traditionally, it is used for treating various conditions such as gastric ulcers, epilepsy, co...
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