Determination of Antioxidant Capacity and HPLC Analysis of Gallic Acid Plus Rutin in Some Lamiaceae Plants Growing in the East of Libya
Abdullah Alamami,
Samar Ben Zaed,
Esra El Naili,
Areej Daboub,
Naema Elremali,
Fatma Elshibani,
Salim Skaik
Issue:
Volume 9, Issue 1, January 2023
Pages:
1-8
Received:
17 November 2022
Accepted:
4 February 2023
Published:
27 February 2023
Abstract: Lamiaceae family comprise a wide range of medicinal and aromatic plant species with strong antioxidant properties and multiple pharmaceutical applications in folk medicine. Mentha piperita L., Ocimum basilicum, Origanum vulgare L, Rosmarinus officinalis L, Salvia officinalis L. and Thymus capitatus, are edible plants in Libya, belonging to this family. The current work aims to evaluate gallic acid and rutin contents of the methanolic extract of the mentioned plants using RP- High performance liquid chromatography (HPLC) and assess their antioxidant properties by applying the DPPH radical scavenging assay. The content of gallic acid were found to be considerable in all plants studied, most remarkably in Rosmarinus officinalis L. and Thymus capitatus with area under the peak (56.14% and 54.62% respectively), while lower contents were detected in Ocimum basilicum (34.44%), Origanum vulgare L. (28.98%), Mentha piperita L.(26.09%), and Salvia officinalis L. (24.34%). On the other hand, it was noted that rutin is found in less quantities compared to previous results. The extracts of Organium vulgare and Thymus capitatus had a significant antioxidant potential compared to standard vitamin C with 0.34 ± 0.03 and 0.46 ± 0.02 mg/mL respectively, while a lower scavenging capacity were recoded for Mentha piperita L. (0.59 mg/mL ± 0.01), Salvia officinalis L. (0.60 mg/mL ± 0.01), Rosmarinus officinalis L (0.65 mg/mL ± 0.07), and Ocimum basilicum (0.99 mg/mL ± 0.02). In conclusion, the reported results point out the significance of this family as a source of antioxidant agents with high promising capability to affect the redox state.
Abstract: Lamiaceae family comprise a wide range of medicinal and aromatic plant species with strong antioxidant properties and multiple pharmaceutical applications in folk medicine. Mentha piperita L., Ocimum basilicum, Origanum vulgare L, Rosmarinus officinalis L, Salvia officinalis L. and Thymus capitatus, are edible plants in Libya, belonging to this fam...
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Effect of Seasonal Variation on the Chemical Composition of Leaf Essential Oil of Aframomum Latifolium (K. Schum) Obtained from Southwest Nigeria
Ogunmola Oluranti Olagoke,
Amusat Mumini Adekunle,
Azeez Eniola Morufat,
Adedosu Taofik Adewale
Issue:
Volume 9, Issue 1, January 2023
Pages:
9-13
Received:
30 November 2022
Accepted:
11 January 2023
Published:
22 May 2023
Abstract: This study was aimed at investigating effect of seasonal variation on yield and chemical composition of leaf essential oil of Aframomum Latifolium over a period of twelve months. Fresh leaves of this plant were subjected to hydrodistillation every month for a period of twelve months using an all glass Clevenger apparatus. The volatile oil obtained each month was analyzed by means of gas chromatography/mass spectrometry (GC/MS). The percentage oil yield ranged between 0.03 – 0.15 % v/w for the twelve treatments. Twenty compounds were identified in the volatile leaf oil obtained over a period of twelve months and oxygenated monoterpene was the most dominant constituent. Cajeputol, an oxygenated monoterpene has the highest percentage in the twelve treatments (39.40 – 45.61%). The effect of seasonal variation was established in the yield of the volatile oil in this study as the maximum yield (0.11%) (v/w) was recorded in November. There is a sudden disappearance of limonene in august and September and a rise in the level of cajeputol between august and November. The volatile oils obtained were rich in cajeputol (45.61%) and α-pinene (20.97%) and these compounds had been documented to be a cough suppressant, anti-inflammatory and anti-microbial agents.
Abstract: This study was aimed at investigating effect of seasonal variation on yield and chemical composition of leaf essential oil of Aframomum Latifolium over a period of twelve months. Fresh leaves of this plant were subjected to hydrodistillation every month for a period of twelve months using an all glass Clevenger apparatus. The volatile oil obtained ...
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