Maternal Age/Delivery Times-specific Reference Intervals of Fibrin Related Biomarkers During Normal Late Pregnancy in Han Population from Southwest China
International Journal of Clinical and Experimental Medical Sciences
Volume 6, Issue 3, May 2020, Pages: 46-50
Received: Jun. 9, 2020;
Accepted: Jun. 23, 2020;
Published: Jun. 29, 2020
Views 19 Downloads 21
Liu Dinghua, Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
Liu Huan, Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
He Qi, Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
Li Xin, Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
Cai Wei, Department of Clinical Laboratory, University-Town Hospital of Chongqing Medical University, Chongqing, China
Objective: Human pregnancy requires hypercoagulation of blood, which means haemostatic reference intervals based on normal people may not be suitable for pregnant women. The aim of this study was to establish reference intervals of fibrin related biomarkers during normal late pregnancy in Han population from southwest China. Methods: Plasma samples were collected from 2374 healthy pregnant women (35-40 gestational weeks) and 326 healthy non-pregnant women at University-Town Hospital of Chongqing Medical University from January 2017 to December 2019. All of the subjects were assigned to 4 groups by age: A (18-29 years), B (30-34 years), C (35-39 years) and D (40-45 years). Further, the pregnant subjects were assigned to 2 groups by delivery times: I (first-delivery) and II (second-delivery). Fibrinogen (Fbg), D-Dimer and fibrin (-ogen) degradation products (FDP) were measured by Sysmex CS-5100 coagulation analyzer. Results: The Fbg reference intervals during late pregnancy in local Han population were 4.09±1.12 g/L. The D-Dimer reference interval for first-delivery pregnancy was <2.77mg/L, and those of second-delivering women were <3.37mg/L (18-34 years) and <3.99mg/L (35-45 years). The FDP reference interval for first-delivery pregnancy was <9.28mg/L, and those of second-delivering women were <10.52mg/L (18-34 years) and <11.72mg/L (35-45 years). Conclusion: In the current study, maternal age/delivery times-specific reference intervals of plasma Fbg, D-Dimer and FDP during normal late pregnancy in Han population from southwest China were presented, which could assist in clinical diagnosis and treatment. Furthermore, we demonstrated maternal age and delivery times did significantly affect the plasma levels of D-dimer and FDP in normal late pregnant women.
Maternal Age/Delivery Times-specific Reference Intervals of Fibrin Related Biomarkers During Normal Late Pregnancy in Han Population from Southwest China, International Journal of Clinical and Experimental Medical Sciences.
Vol. 6, No. 3,
2020, pp. 46-50.
The death rate of Chinese pregnant women increased: or because that age-advanced women risked lives with second delivery http://news.163.com/16/1006/00/C2LFF2DM00014JB5.html (accessed 13 November 2016).
Kristoffersen AH., Petersen PH., RΦraas T. and Sandberg S. (2017). Estimates of within-subject biological variation of protein C, antithrombin, protein S free, protein S activity, and activated protein C resistance in pregnant women. Clinical Chemistry, 63 (4): 898-907.
Parunov LA., Soshitova NP., Ovanesov MV., Panteleev MA. And Serebriyskiy II. (2015). Epidemiology of venous thromboembolism associated with pregnancy. Birth Defects Research part C Embryo Today, 105 (3): 167-184.
Heavner MS., Mansouri R., Arndt M., Parkeret L. and Eyler RF. (2017). Thrombolysis for massive pulmonary embolism in pregnancy. Pharmacotherapy, 37 (11): 1449-1457.
Othman M., McLintock C. and Kadir R.. (2016). Thrombosis and Hemostasis Related Issues in Women and Pregnancy. Seminars in Thrombosis and Hemostasis, 42 (7): 693-695.
Olson JD. (2015). D-dimer: An Overview of Hemostasis and Fibrinolysis, Assays, and Clinical Applications. Advances in Clinical Chemistry, 69 (1): 1-6.
Riva N., Vella K., Hickey K., Bertu L., Zammit D., and Spiteri S. (2018). Biomarkers for the diagnosis of venous thromboembolism: D-dimer, thrombin generation, procoagulant phospholipid and soluble P-selectin. Journal of Clinical Pathology, 71 (11): 1015-1022.
Xie X., and Gou WL, Obstetrics and Gynaecology, 8th ed. People’s Medical Publishing House: Beijing, 2014, pp. 221-2.
Antony KM., Mansouri R., Arndt M., Hui SK., Jariwala P., and Mcmullen VM. (2015). Establishing thromboelastography with platelet-function analyzer reference ranges and other measures in healthy term pregnant women. American Journal of Perinatology, 32 (6): 545-554.
Stanciakova L., Dobrotova M., Jedinakova Z., Duraj L., Skornova I. and Korinkova L. (2016). Monitoring of hemostasis and management of anticoagulant thromboprophylaxis in pregnant women with increased risk of fetal loss. Seminars in Thrombosis and Hemostasis, 42 (6): 612-621.
Morikawa M., Yamada T., Akaishi R., Akaishi R., Koyama R. and Minakami H. (2011). Changes in D-dimer levels after cesarean section in women with singleton and twin pregnancies. Thrombosis Research, 128 (4): 33-38.
Sekiya A., Hayashi T., Kadohira Y., Shibayama M., Tsuda I., and Jin X. (2017). Thrombosis prediction based on reference ranges of coagulation-related markers in different stages of pregnancy. Clinical and Applied Thrombosis/ Hemostasis, 23 (7): 844-850.
CLSI, Defining, establishing, and verifying of reference intervals in the clinical laboratory: Approved guideline, C28-A3E. 3rd ed. Wayne: Clinical and Laboratory Standards Institute, 2008, pp. 1-4.
Gong JM., Shen Y. and He YX. (2016). Reference inervals of routine coagulation assays during the pregnancy and puerperium period. Journal of Clinical Laboratory Analysis, 30 (6): 912-917.
Gutiérrez GI., Pérez CP., Martínez UJ., Izquierdo OG., Pérez MA., and Romualdo LG. (2018). D-dimer during pregnancy: establishing trimester-specific reference intervals. Scandinavian Journal of Clinical Laboratory Investigation, 78 (6): 439-442.
Cui C., Yang S., Zhang J., WangG., Huang S. and Li A. (2017). Trimester-specific coagulation and anticoagulation reference intervals for healthy pregnancy. Thrombosis Research, 156 (1): 82-86.