A new model of Cascaded Black – Linear Distribution (CBLD) in circular aperture is suggested. Four different models of CBLD are studied. In the 1st model, ten strips are considered where half of them are black and the other half has linear distribution, starting with black strip from the center of the circular aperture. In the 2nd model, twenty strips are considered of equal black and linear zones. In the 3rd model, a ratio of 2:1 is given for the twenty strips of the CBLD. In the 4th model, annular aperture of linear distribution is considered. We have computed the Point Spread Function (PSF) corresponding to all arrangements and compared with the corresponding PSF for different apertures of circular, annular, and black and white (B/W) transparent circular apertures. The cut-off spatial frequency which is the indication of resolution is investigated in all the described apertures. The Coherent Transfer Function (CTF) using the CBLD apertures is computed. Application of the CBLD arrangement corresponding to the objective and collector lenses in the CLSM using microscopic input images is shown. The reconstructed images using the described models in the CLSM are investigated. A Mat-Lab code is used for the computation of all images.
| Published in | American Journal of Optics and Photonics (Volume 7, Issue 3) |
| DOI | 10.11648/j.ajop.20190703.11 |
| Page(s) | 46-56 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Modulated Apertures, Resolution, Confocal Laser Scanning Microscope (CLSM)
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APA Style
Abdallah Mohamed Hamed. (2019). Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM). American Journal of Optics and Photonics, 7(3), 46-56. https://doi.org/10.11648/j.ajop.20190703.11
ACS Style
Abdallah Mohamed Hamed. Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM). Am. J. Opt. Photonics 2019, 7(3), 46-56. doi: 10.11648/j.ajop.20190703.11
AMA Style
Abdallah Mohamed Hamed. Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM). Am J Opt Photonics. 2019;7(3):46-56. doi: 10.11648/j.ajop.20190703.11
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Download@article{10.11648/j.ajop.20190703.11,
author = {Abdallah Mohamed Hamed},
title = {Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM)},
journal = {American Journal of Optics and Photonics},
volume = {7},
number = {3},
pages = {46-56},
doi = {10.11648/j.ajop.20190703.11},
url = {https://doi.org/10.11648/j.ajop.20190703.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajop.20190703.11},
abstract = {A new model of Cascaded Black – Linear Distribution (CBLD) in circular aperture is suggested. Four different models of CBLD are studied. In the 1st model, ten strips are considered where half of them are black and the other half has linear distribution, starting with black strip from the center of the circular aperture. In the 2nd model, twenty strips are considered of equal black and linear zones. In the 3rd model, a ratio of 2:1 is given for the twenty strips of the CBLD. In the 4th model, annular aperture of linear distribution is considered. We have computed the Point Spread Function (PSF) corresponding to all arrangements and compared with the corresponding PSF for different apertures of circular, annular, and black and white (B/W) transparent circular apertures. The cut-off spatial frequency which is the indication of resolution is investigated in all the described apertures. The Coherent Transfer Function (CTF) using the CBLD apertures is computed. Application of the CBLD arrangement corresponding to the objective and collector lenses in the CLSM using microscopic input images is shown. The reconstructed images using the described models in the CLSM are investigated. A Mat-Lab code is used for the computation of all images.},
year = {2019}
}
TY - JOUR T1 - Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM) AU - Abdallah Mohamed Hamed Y1 - 2019/10/09 PY - 2019 N1 - https://doi.org/10.11648/j.ajop.20190703.11 DO - 10.11648/j.ajop.20190703.11 T2 - American Journal of Optics and Photonics JF - American Journal of Optics and Photonics JO - American Journal of Optics and Photonics SP - 46 EP - 56 PB - Science Publishing Group SN - 2330-8494 UR - https://doi.org/10.11648/j.ajop.20190703.11 AB - A new model of Cascaded Black – Linear Distribution (CBLD) in circular aperture is suggested. Four different models of CBLD are studied. In the 1st model, ten strips are considered where half of them are black and the other half has linear distribution, starting with black strip from the center of the circular aperture. In the 2nd model, twenty strips are considered of equal black and linear zones. In the 3rd model, a ratio of 2:1 is given for the twenty strips of the CBLD. In the 4th model, annular aperture of linear distribution is considered. We have computed the Point Spread Function (PSF) corresponding to all arrangements and compared with the corresponding PSF for different apertures of circular, annular, and black and white (B/W) transparent circular apertures. The cut-off spatial frequency which is the indication of resolution is investigated in all the described apertures. The Coherent Transfer Function (CTF) using the CBLD apertures is computed. Application of the CBLD arrangement corresponding to the objective and collector lenses in the CLSM using microscopic input images is shown. The reconstructed images using the described models in the CLSM are investigated. A Mat-Lab code is used for the computation of all images. VL - 7 IS - 3 ER -
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