Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Noritaka Yamamoto

doi:doi:10.11648/j.ejb.20170501.11

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Tensile Strength of Single Collagen Fibrils Isolated from Tendons

Noritaka Yamamoto

Published in European Journal of Biophysics (Volume 5, Issue 1)

Received: 28 November 2016 Accepted: 7 January 2017 Published: 16 February 2017

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Abstract

Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.

Published in	European Journal of Biophysics (Volume 5, Issue 1)
DOI	10.11648/j.ejb.20170501.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Collagen, Fibril, Tendon, Mechanical Properties, Tensile Strength

References

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Noritaka Yamamoto. (2017). Tensile Strength of Single Collagen Fibrils Isolated from Tendons. European Journal of Biophysics, 5(1), 1-6. https://doi.org/10.11648/j.ejb.20170501.11

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ACS Style

Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur. J. Biophys. 2017, 5(1), 1-6. doi: 10.11648/j.ejb.20170501.11

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AMA Style

Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur J Biophys. 2017;5(1):1-6. doi: 10.11648/j.ejb.20170501.11

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@article{10.11648/j.ejb.20170501.11,
  author = {Noritaka Yamamoto},
  title = {Tensile Strength of Single Collagen Fibrils Isolated from Tendons},
  journal = {European Journal of Biophysics},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ejb.20170501.11},
  url = {https://doi.org/10.11648/j.ejb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20170501.11},
  abstract = {Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.},
 year = {2017}
}

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TY  - JOUR
T1  - Tensile Strength of Single Collagen Fibrils Isolated from Tendons
AU  - Noritaka Yamamoto
Y1  - 2017/02/16
PY  - 2017
N1  - https://doi.org/10.11648/j.ejb.20170501.11
DO  - 10.11648/j.ejb.20170501.11
T2  - European Journal of Biophysics
JF  - European Journal of Biophysics
JO  - European Journal of Biophysics
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-1737
UR  - https://doi.org/10.11648/j.ejb.20170501.11
AB  - Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.
VL  - 5
IS  - 1
ER  -

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Author Information

Noritaka Yamamoto

Biomechanics Laboratory, Department of Mechanical Engineering, Ritsumeikan University, Shiga, Japan

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Plain Text BibTeX RIS

APA Style

Noritaka Yamamoto. (2017). Tensile Strength of Single Collagen Fibrils Isolated from Tendons. European Journal of Biophysics, 5(1), 1-6. https://doi.org/10.11648/j.ejb.20170501.11

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ACS Style

Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur. J. Biophys. 2017, 5(1), 1-6. doi: 10.11648/j.ejb.20170501.11

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AMA Style

Noritaka Yamamoto. Tensile Strength of Single Collagen Fibrils Isolated from Tendons. Eur J Biophys. 2017;5(1):1-6. doi: 10.11648/j.ejb.20170501.11

Copy | Download

@article{10.11648/j.ejb.20170501.11,
  author = {Noritaka Yamamoto},
  title = {Tensile Strength of Single Collagen Fibrils Isolated from Tendons},
  journal = {European Journal of Biophysics},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ejb.20170501.11},
  url = {https://doi.org/10.11648/j.ejb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20170501.11},
  abstract = {Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.},
 year = {2017}
}

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TY  - JOUR
T1  - Tensile Strength of Single Collagen Fibrils Isolated from Tendons
AU  - Noritaka Yamamoto
Y1  - 2017/02/16
PY  - 2017
N1  - https://doi.org/10.11648/j.ejb.20170501.11
DO  - 10.11648/j.ejb.20170501.11
T2  - European Journal of Biophysics
JF  - European Journal of Biophysics
JO  - European Journal of Biophysics
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-1737
UR  - https://doi.org/10.11648/j.ejb.20170501.11
AB  - Tensile failure properties of single collagen fibrils were determined using our original tensile test method. Fibrils were directly isolated from the fascicles of mouse tail tendons. Both the ends of each fibril were wound onto the tips of two microneedles several times using micromanipulators. The fibril and tips were immersed in physiological saline solution. Then, the fibril was stretched to failure by moving the one microneedle. During tensile testing, the fibril was firmly attached to the tips of the microneedles, and broken between the tips with no slippage observed. The diameter of tested 10 fibrils was 410±60 nm (Mean±S.D.). The stress-strain curves of these fibrils were almost linear. Their tensile strength and failure strain were 100±32 MPa and 34±11%, respectively. These values were approximately 480 and 190% of those of the fascicles with the diameter of 81±12 m, respectively.
VL  - 5
IS  - 1
ER  -

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