Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants

Ayman Ahmed; Mervat Ahmed Ali; Mona Mostafa Gamal; Abdel Sattar Mohamed Sallam; Elsayed Mahmoud Elsayed

doi:doi:10.11648/j.ejb.20200802.12

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Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants

Ayman Ahmed, Mervat Ahmed Ali, Mona Mostafa Gamal, Abdel Sattar Mohamed Sallam, Elsayed Mahmoud Elsayed

Published in European Journal of Biophysics (Volume 8, Issue 2)

Received: 19 August 2020 Accepted: 5 September 2020 Published: 24 September 2020

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Abstract

Background: Applications of ultrasound in medicine for therapeutic purposes have been an accepted and beneficial use of ultrasonic biological effects for many years but exposure to ultrasound can generate oxidative stress. Aim: The aim of the present study is to investigate the changes in the function of the retina due to ultrasound exposure and the protecting role of vitamin C and/or β-carotene by using electroretinogram (ERG). Materials and Methods: Newzealand albino rabbits of both sexes classified into five groups. Group (I) used as control group. The eyes of group (II) Insonificated with continuous ultrasound waves (1.5 W/cm² at 2.8 MHz) for 20, 40 and 60 minutes. Group (III) was insonificated with vitamin C instillation and group (IV) insonificated after -carotene supplementation. Group (V) was insonificated after combination of the two treatments. Results: Statistically significant reduction (P˂ 0.05) in a- waves amplitude after insonification and exceeding with increase exposure time 20, 40 and 60 min was observed. The amplitudes of a-wave after treatment with topical and supplemented or both remained significantly larger compared with those exposure to US only but its latency lower than that found in insonified groups. In addition, the b-waves were found to follow the same behavior as a-wave. Conclusion: The study recommended considering oral β-carotene antioxidant in combination with vitamin C eye drops as a medical tool and personal protective in ultrasound equipment in provide safety.

Published in	European Journal of Biophysics (Volume 8, Issue 2)
DOI	10.11648/j.ejb.20200802.12
Page(s)	20-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Ultrasound, Vitamin C, β-carotene, Electroretinogram, Retina, Rabbit

References

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APA Style

Ayman Ahmed, Mervat Ahmed Ali, Mona Mostafa Gamal, Abdel Sattar Mohamed Sallam, Elsayed Mahmoud Elsayed. (2020). Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. European Journal of Biophysics, 8(2), 20-25. https://doi.org/10.11648/j.ejb.20200802.12

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ACS Style

Ayman Ahmed; Mervat Ahmed Ali; Mona Mostafa Gamal; Abdel Sattar Mohamed Sallam; Elsayed Mahmoud Elsayed. Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. Eur. J. Biophys. 2020, 8(2), 20-25. doi: 10.11648/j.ejb.20200802.12

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AMA Style

Ayman Ahmed, Mervat Ahmed Ali, Mona Mostafa Gamal, Abdel Sattar Mohamed Sallam, Elsayed Mahmoud Elsayed. Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. Eur J Biophys. 2020;8(2):20-25. doi: 10.11648/j.ejb.20200802.12

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@article{10.11648/j.ejb.20200802.12,
  author = {Ayman Ahmed and Mervat Ahmed Ali and Mona Mostafa Gamal and Abdel Sattar Mohamed Sallam and Elsayed Mahmoud Elsayed},
  title = {Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants},
  journal = {European Journal of Biophysics},
  volume = {8},
  number = {2},
  pages = {20-25},
  doi = {10.11648/j.ejb.20200802.12},
  url = {https://doi.org/10.11648/j.ejb.20200802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20200802.12},
  abstract = {Background: Applications of ultrasound in medicine for therapeutic purposes have been an accepted and beneficial use of ultrasonic biological effects for many years but exposure to ultrasound can generate oxidative stress. Aim: The aim of the present study is to investigate the changes in the function of the retina due to ultrasound exposure and the protecting role of vitamin C and/or β-carotene by using electroretinogram (ERG). Materials and Methods: Newzealand albino rabbits of both sexes classified into five groups. Group (I) used as control group. The eyes of group (II) Insonificated with continuous ultrasound waves (1.5 W/cm2 at 2.8 MHz) for 20, 40 and 60 minutes. Group (III) was insonificated with vitamin C instillation and group (IV) insonificated after -carotene supplementation. Group (V) was insonificated after combination of the two treatments. Results: Statistically significant reduction (P˂ 0.05) in a- waves amplitude after insonification and exceeding with increase exposure time 20, 40 and 60 min was observed. The amplitudes of a-wave after treatment with topical and supplemented or both remained significantly larger compared with those exposure to US only but its latency lower than that found in insonified groups. In addition, the b-waves were found to follow the same behavior as a-wave. Conclusion: The study recommended considering oral β-carotene antioxidant in combination with vitamin C eye drops as a medical tool and personal protective in ultrasound equipment in provide safety.},
 year = {2020}
}

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TY - JOUR
T1 - Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants
AU - Ayman Ahmed
AU - Mervat Ahmed Ali
AU - Mona Mostafa Gamal
AU - Abdel Sattar Mohamed Sallam
AU - Elsayed Mahmoud Elsayed
Y1 - 2020/09/24
PY - 2020
N1 - https://doi.org/10.11648/j.ejb.20200802.12
DO - 10.11648/j.ejb.20200802.12
T2 - European Journal of Biophysics
JF - European Journal of Biophysics
JO - European Journal of Biophysics
SP - 20
EP - 25
PB - Science Publishing Group
SN - 2329-1737
UR - https://doi.org/10.11648/j.ejb.20200802.12
AB - Background: Applications of ultrasound in medicine for therapeutic purposes have been an accepted and beneficial use of ultrasonic biological effects for many years but exposure to ultrasound can generate oxidative stress. Aim: The aim of the present study is to investigate the changes in the function of the retina due to ultrasound exposure and the protecting role of vitamin C and/or β-carotene by using electroretinogram (ERG). Materials and Methods: Newzealand albino rabbits of both sexes classified into five groups. Group (I) used as control group. The eyes of group (II) Insonificated with continuous ultrasound waves (1.5 W/cm2 at 2.8 MHz) for 20, 40 and 60 minutes. Group (III) was insonificated with vitamin C instillation and group (IV) insonificated after -carotene supplementation. Group (V) was insonificated after combination of the two treatments. Results: Statistically significant reduction (P˂ 0.05) in a- waves amplitude after insonification and exceeding with increase exposure time 20, 40 and 60 min was observed. The amplitudes of a-wave after treatment with topical and supplemented or both remained significantly larger compared with those exposure to US only but its latency lower than that found in insonified groups. In addition, the b-waves were found to follow the same behavior as a-wave. Conclusion: The study recommended considering oral β-carotene antioxidant in combination with vitamin C eye drops as a medical tool and personal protective in ultrasound equipment in provide safety.
VL - 8
IS - 2
ER -

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Author Information

Ayman Ahmed

Collage of Science, Ain Shams University, Cairo, Egypt
Mervat Ahmed Ali

Research Institute of Ophthalmology, Giza, Egypt
Mona Mostafa Gamal

Research Institute of Ophthalmology, Giza, Egypt
Abdel Sattar Mohamed Sallam

Collage of Science, Ain Shams University, Cairo, Egypt
Elsayed Mahmoud Elsayed

Collage of Science, Ain Shams University, Cairo, Egypt

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Ayman Ahmed, Mervat Ahmed Ali, Mona Mostafa Gamal, Abdel Sattar Mohamed Sallam, Elsayed Mahmoud Elsayed. (2020). Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. European Journal of Biophysics, 8(2), 20-25. https://doi.org/10.11648/j.ejb.20200802.12

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ACS Style

Ayman Ahmed; Mervat Ahmed Ali; Mona Mostafa Gamal; Abdel Sattar Mohamed Sallam; Elsayed Mahmoud Elsayed. Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. Eur. J. Biophys. 2020, 8(2), 20-25. doi: 10.11648/j.ejb.20200802.12

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AMA Style

Ayman Ahmed, Mervat Ahmed Ali, Mona Mostafa Gamal, Abdel Sattar Mohamed Sallam, Elsayed Mahmoud Elsayed. Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants. Eur J Biophys. 2020;8(2):20-25. doi: 10.11648/j.ejb.20200802.12

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@article{10.11648/j.ejb.20200802.12,
  author = {Ayman Ahmed and Mervat Ahmed Ali and Mona Mostafa Gamal and Abdel Sattar Mohamed Sallam and Elsayed Mahmoud Elsayed},
  title = {Potential Adverse Effect of Ultrasound on the Electroretinogram of Retina and the Protective Role of Different Types of Antioxidants},
  journal = {European Journal of Biophysics},
  volume = {8},
  number = {2},
  pages = {20-25},
  doi = {10.11648/j.ejb.20200802.12},
  url = {https://doi.org/10.11648/j.ejb.20200802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20200802.12},
  abstract = {Background: Applications of ultrasound in medicine for therapeutic purposes have been an accepted and beneficial use of ultrasonic biological effects for many years but exposure to ultrasound can generate oxidative stress. Aim: The aim of the present study is to investigate the changes in the function of the retina due to ultrasound exposure and the protecting role of vitamin C and/or β-carotene by using electroretinogram (ERG). Materials and Methods: Newzealand albino rabbits of both sexes classified into five groups. Group (I) used as control group. The eyes of group (II) Insonificated with continuous ultrasound waves (1.5 W/cm2 at 2.8 MHz) for 20, 40 and 60 minutes. Group (III) was insonificated with vitamin C instillation and group (IV) insonificated after -carotene supplementation. Group (V) was insonificated after combination of the two treatments. Results: Statistically significant reduction (P˂ 0.05) in a- waves amplitude after insonification and exceeding with increase exposure time 20, 40 and 60 min was observed. The amplitudes of a-wave after treatment with topical and supplemented or both remained significantly larger compared with those exposure to US only but its latency lower than that found in insonified groups. In addition, the b-waves were found to follow the same behavior as a-wave. Conclusion: The study recommended considering oral β-carotene antioxidant in combination with vitamin C eye drops as a medical tool and personal protective in ultrasound equipment in provide safety.},
 year = {2020}
}

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