Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts

Asomidin Faizovich Sadriddinov; Sheraliev Kambarali Saidalievich; Hidirova Gulnoza Azodovna; Muborak Asomidinovna Sadriddinova; Elena Nikolaevna Guzacheva

doi:doi:10.11648/j.ijcems.20210704.15

Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts

Asomidin Faizovich Sadriddinov, Sheraliev Kambarali Saidalievich, Hidirova Gulnoza Azodovna, Muborak Asomidinovna Sadriddinova, Elena Nikolaevna Guzacheva

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)

Received: 8 June 2021 Accepted: 8 July 2021 Published: 24 July 2021

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Abstract

On an experimental model of obstructive jaundice in experimental rats, the cytological aspects of proliferation and the possibility of transformation of cholangiol cells into hepatocytes, as well as the cellular mechanisms of involution of the bile ducts, were investigated. The material was the liver of sexually mature rats (n=38) with 10, 20, and 25 days of cholestasis, in which the duct was recanalized during these periods and sacrificed on the 5th, 10th, 15th, 30th and 90th days after the second operation. Pieces of liver tissue were processed by conventional histological and electron microscopic methods. Ligation of the bile duct promotes rapid proliferation of the bile ducts, which correlates with the duration of cholestasis. In the early stages of the experiment (cholestasis for 10 days), the bile ducts occupy the peripheral sections of the lobules, by the 20th day, cholestasis penetrates deeply into the lobule, dismembering it into fragments, and by the 25th day, a picture of biliary cirrhosis develops. It was found that the proliferation of the bile ducts occurs due to the duct cells themselves, the transformation of cholangiol cells into "oval" cells or hepatocytes was not found. The transformation criteria were the shape, intracellular structures, and the state of the boundary plate and the basement membrane of the ducts. Consequently, bile duct cells (cholangioli) and hepatocytes, diverse in their function, showed absolute genetic heterogeneity. After the restoration of the outflow of bile, non-functioning ducts undergo destruction and decay. It has been shown that hepatic macrophages play an active role in cleansing the liver parenchyma from the products of cellular decay of the bile ducts, dead liver cells, as well as excess fibrillar structures. Activated Kupffer cells, due to the content of a large number of heterolysosomes, provide degradation, lysis and utilization of cellular and extracellular structures.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)
DOI	10.11648/j.ijcems.20210704.15
Page(s)	98-102
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Obstructive Jaundice, Liver, Proliferation, Transformation and Involution of Cells

References

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[7]	Sadriddinov, A. F., Abdullaeva N. E., Sadriddinova M. A., Fakhriev J. A., Shoyunusov S. I. The classic hepatic lobule is in three dimensions: organization, cytoacrchitecture and hem circulatory mainstream of simple hepatic lobule for rat and human. European Science Review, Austria, and Vienna. 2017, № 5-6, p. 36-41.
[8]	Kashaeva M. D., Proshin A. V., Afanasyev A. N., Gavrilova K. V., Golushko A. V. Liver morphology in obstructive jaundice. Bulletin of Novgorod State University, 2018; No. 6 (112). P. 8-11.
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[10]	Parent R., Marion M. J., Furio L. et al. Origin and characterization of a human bipotent liver progenitor cell line. Gastroenterology 2004; 126 (4): 1147-56.
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[12]	Kiyasov A. P., Gumerova A. A., Titova M. A. Oval cells putative liver stem cells or hepatoblasts? Cell transplantology and tissue engineering. 2006, No. 2 (4), - C, 55-58.
[13]	Engelhardt N. V., Factor V. M., Medvinsky A. L. et al. Common antigen of oval and biliary epithelial cells (A6) is a differentiation marker of epithelial and erythroid cell lineages in early development of the mouse. Differentiation 1993; 55: - P. 19-26.
[14]	Klein I., Cornejo J. C., Polakos N. K., John B., Wuensch S. A., Topham D. J. Pierce R. N., Crispe I. N. Kupffer cell heterogeneity: functional properties of bone marrow derived and sessile hepatic macrophages. Blood. – 2007. -Vol. 110 (12). – P. 4077-4085.
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Asomidin Faizovich Sadriddinov, Sheraliev Kambarali Saidalievich, Hidirova Gulnoza Azodovna, Muborak Asomidinovna Sadriddinova, Elena Nikolaevna Guzacheva. (2021). Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. International Journal of Clinical and Experimental Medical Sciences, 7(4), 98-102. https://doi.org/10.11648/j.ijcems.20210704.15

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ACS Style

Asomidin Faizovich Sadriddinov; Sheraliev Kambarali Saidalievich; Hidirova Gulnoza Azodovna; Muborak Asomidinovna Sadriddinova; Elena Nikolaevna Guzacheva. Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 98-102. doi: 10.11648/j.ijcems.20210704.15

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AMA Style

Asomidin Faizovich Sadriddinov, Sheraliev Kambarali Saidalievich, Hidirova Gulnoza Azodovna, Muborak Asomidinovna Sadriddinova, Elena Nikolaevna Guzacheva. Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. Int J Clin Exp Med Sci. 2021;7(4):98-102. doi: 10.11648/j.ijcems.20210704.15

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@article{10.11648/j.ijcems.20210704.15,
  author = {Asomidin Faizovich Sadriddinov and Sheraliev Kambarali Saidalievich and Hidirova Gulnoza Azodovna and Muborak Asomidinovna Sadriddinova and Elena Nikolaevna Guzacheva},
  title = {Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {98-102},
  doi = {10.11648/j.ijcems.20210704.15},
  url = {https://doi.org/10.11648/j.ijcems.20210704.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.15},
  abstract = {On an experimental model of obstructive jaundice in experimental rats, the cytological aspects of proliferation and the possibility of transformation of cholangiol cells into hepatocytes, as well as the cellular mechanisms of involution of the bile ducts, were investigated. The material was the liver of sexually mature rats (n=38) with 10, 20, and 25 days of cholestasis, in which the duct was recanalized during these periods and sacrificed on the 5th, 10th, 15th, 30th and 90th days after the second operation. Pieces of liver tissue were processed by conventional histological and electron microscopic methods. Ligation of the bile duct promotes rapid proliferation of the bile ducts, which correlates with the duration of cholestasis. In the early stages of the experiment (cholestasis for 10 days), the bile ducts occupy the peripheral sections of the lobules, by the 20th day, cholestasis penetrates deeply into the lobule, dismembering it into fragments, and by the 25th day, a picture of biliary cirrhosis develops. It was found that the proliferation of the bile ducts occurs due to the duct cells themselves, the transformation of cholangiol cells into "oval" cells or hepatocytes was not found. The transformation criteria were the shape, intracellular structures, and the state of the boundary plate and the basement membrane of the ducts. Consequently, bile duct cells (cholangioli) and hepatocytes, diverse in their function, showed absolute genetic heterogeneity. After the restoration of the outflow of bile, non-functioning ducts undergo destruction and decay. It has been shown that hepatic macrophages play an active role in cleansing the liver parenchyma from the products of cellular decay of the bile ducts, dead liver cells, as well as excess fibrillar structures. Activated Kupffer cells, due to the content of a large number of heterolysosomes, provide degradation, lysis and utilization of cellular and extracellular structures.},
 year = {2021}
}

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TY  - JOUR
T1  - Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts
AU  - Asomidin Faizovich Sadriddinov
AU  - Sheraliev Kambarali Saidalievich
AU  - Hidirova Gulnoza Azodovna
AU  - Muborak Asomidinovna Sadriddinova
AU  - Elena Nikolaevna Guzacheva
Y1  - 2021/07/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210704.15
DO  - 10.11648/j.ijcems.20210704.15
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 98
EP  - 102
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210704.15
AB  - On an experimental model of obstructive jaundice in experimental rats, the cytological aspects of proliferation and the possibility of transformation of cholangiol cells into hepatocytes, as well as the cellular mechanisms of involution of the bile ducts, were investigated. The material was the liver of sexually mature rats (n=38) with 10, 20, and 25 days of cholestasis, in which the duct was recanalized during these periods and sacrificed on the 5th, 10th, 15th, 30th and 90th days after the second operation. Pieces of liver tissue were processed by conventional histological and electron microscopic methods. Ligation of the bile duct promotes rapid proliferation of the bile ducts, which correlates with the duration of cholestasis. In the early stages of the experiment (cholestasis for 10 days), the bile ducts occupy the peripheral sections of the lobules, by the 20th day, cholestasis penetrates deeply into the lobule, dismembering it into fragments, and by the 25th day, a picture of biliary cirrhosis develops. It was found that the proliferation of the bile ducts occurs due to the duct cells themselves, the transformation of cholangiol cells into "oval" cells or hepatocytes was not found. The transformation criteria were the shape, intracellular structures, and the state of the boundary plate and the basement membrane of the ducts. Consequently, bile duct cells (cholangioli) and hepatocytes, diverse in their function, showed absolute genetic heterogeneity. After the restoration of the outflow of bile, non-functioning ducts undergo destruction and decay. It has been shown that hepatic macrophages play an active role in cleansing the liver parenchyma from the products of cellular decay of the bile ducts, dead liver cells, as well as excess fibrillar structures. Activated Kupffer cells, due to the content of a large number of heterolysosomes, provide degradation, lysis and utilization of cellular and extracellular structures.
VL  - 7
IS  - 4
ER  -

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Author Information

Asomidin Faizovich Sadriddinov

Department of Histology and Pathological Physiology, Tashkent Pediatric Medical Institute, Tashkent, Uzbekistan
Sheraliev Kambarali Saidalievich

Department of Anatomy and Pathological Anatomy, Tashkent Pediatric Medical Institute, Tashkent, Uzbekistan
Hidirova Gulnoza Azodovna

Department of Anatomy and Pathological Anatomy, Tashkent Pediatric Medical Institute, Tashkent, Uzbekistan
Muborak Asomidinovna Sadriddinova

Department of Emergency Pediatrics, Tashkent Pediatric Medical Institute, Tashkent, Uzbekistan
Elena Nikolaevna Guzacheva

“Xayat” – Private Clinic, Tashkent, Uzbekistan

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APA Style

Asomidin Faizovich Sadriddinov, Sheraliev Kambarali Saidalievich, Hidirova Gulnoza Azodovna, Muborak Asomidinovna Sadriddinova, Elena Nikolaevna Guzacheva. (2021). Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. International Journal of Clinical and Experimental Medical Sciences, 7(4), 98-102. https://doi.org/10.11648/j.ijcems.20210704.15

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ACS Style

Asomidin Faizovich Sadriddinov; Sheraliev Kambarali Saidalievich; Hidirova Gulnoza Azodovna; Muborak Asomidinovna Sadriddinova; Elena Nikolaevna Guzacheva. Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 98-102. doi: 10.11648/j.ijcems.20210704.15

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AMA Style

Asomidin Faizovich Sadriddinov, Sheraliev Kambarali Saidalievich, Hidirova Gulnoza Azodovna, Muborak Asomidinovna Sadriddinova, Elena Nikolaevna Guzacheva. Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts. Int J Clin Exp Med Sci. 2021;7(4):98-102. doi: 10.11648/j.ijcems.20210704.15

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@article{10.11648/j.ijcems.20210704.15,
  author = {Asomidin Faizovich Sadriddinov and Sheraliev Kambarali Saidalievich and Hidirova Gulnoza Azodovna and Muborak Asomidinovna Sadriddinova and Elena Nikolaevna Guzacheva},
  title = {Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {98-102},
  doi = {10.11648/j.ijcems.20210704.15},
  url = {https://doi.org/10.11648/j.ijcems.20210704.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.15},
  abstract = {On an experimental model of obstructive jaundice in experimental rats, the cytological aspects of proliferation and the possibility of transformation of cholangiol cells into hepatocytes, as well as the cellular mechanisms of involution of the bile ducts, were investigated. The material was the liver of sexually mature rats (n=38) with 10, 20, and 25 days of cholestasis, in which the duct was recanalized during these periods and sacrificed on the 5th, 10th, 15th, 30th and 90th days after the second operation. Pieces of liver tissue were processed by conventional histological and electron microscopic methods. Ligation of the bile duct promotes rapid proliferation of the bile ducts, which correlates with the duration of cholestasis. In the early stages of the experiment (cholestasis for 10 days), the bile ducts occupy the peripheral sections of the lobules, by the 20th day, cholestasis penetrates deeply into the lobule, dismembering it into fragments, and by the 25th day, a picture of biliary cirrhosis develops. It was found that the proliferation of the bile ducts occurs due to the duct cells themselves, the transformation of cholangiol cells into "oval" cells or hepatocytes was not found. The transformation criteria were the shape, intracellular structures, and the state of the boundary plate and the basement membrane of the ducts. Consequently, bile duct cells (cholangioli) and hepatocytes, diverse in their function, showed absolute genetic heterogeneity. After the restoration of the outflow of bile, non-functioning ducts undergo destruction and decay. It has been shown that hepatic macrophages play an active role in cleansing the liver parenchyma from the products of cellular decay of the bile ducts, dead liver cells, as well as excess fibrillar structures. Activated Kupffer cells, due to the content of a large number of heterolysosomes, provide degradation, lysis and utilization of cellular and extracellular structures.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Experimental Mechanical Jaundice: Morphological Aspects of Proliferation, the Possibility of Transformation and Involution of Epithelial Cells in the Bile Ducts
AU  - Asomidin Faizovich Sadriddinov
AU  - Sheraliev Kambarali Saidalievich
AU  - Hidirova Gulnoza Azodovna
AU  - Muborak Asomidinovna Sadriddinova
AU  - Elena Nikolaevna Guzacheva
Y1  - 2021/07/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210704.15
DO  - 10.11648/j.ijcems.20210704.15
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 98
EP  - 102
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210704.15
AB  - On an experimental model of obstructive jaundice in experimental rats, the cytological aspects of proliferation and the possibility of transformation of cholangiol cells into hepatocytes, as well as the cellular mechanisms of involution of the bile ducts, were investigated. The material was the liver of sexually mature rats (n=38) with 10, 20, and 25 days of cholestasis, in which the duct was recanalized during these periods and sacrificed on the 5th, 10th, 15th, 30th and 90th days after the second operation. Pieces of liver tissue were processed by conventional histological and electron microscopic methods. Ligation of the bile duct promotes rapid proliferation of the bile ducts, which correlates with the duration of cholestasis. In the early stages of the experiment (cholestasis for 10 days), the bile ducts occupy the peripheral sections of the lobules, by the 20th day, cholestasis penetrates deeply into the lobule, dismembering it into fragments, and by the 25th day, a picture of biliary cirrhosis develops. It was found that the proliferation of the bile ducts occurs due to the duct cells themselves, the transformation of cholangiol cells into "oval" cells or hepatocytes was not found. The transformation criteria were the shape, intracellular structures, and the state of the boundary plate and the basement membrane of the ducts. Consequently, bile duct cells (cholangioli) and hepatocytes, diverse in their function, showed absolute genetic heterogeneity. After the restoration of the outflow of bile, non-functioning ducts undergo destruction and decay. It has been shown that hepatic macrophages play an active role in cleansing the liver parenchyma from the products of cellular decay of the bile ducts, dead liver cells, as well as excess fibrillar structures. Activated Kupffer cells, due to the content of a large number of heterolysosomes, provide degradation, lysis and utilization of cellular and extracellular structures.
VL  - 7
IS  - 4
ER  -

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