New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy

Hao Song; Hongyan Ma; Yao Huang; Bin Yi; Xiaoqing Jiang

doi:doi:10.11648/j.ijcems.20210704.14

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New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy

Hao Song, Hongyan Ma, Yao Huang, Bin Yi, Xiaoqing Jiang

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)

Received: 20 June 2021 Accepted: 8 July 2021 Published: 16 July 2021

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Abstract

Objective: To explore the common mutation gene and mutation rule of cholangiocarcinoma and gallbladder cancer, and to confirm the therapeutic effect of PD1 inhibitor through follow-up. Method: From 2017 to 2019, 42 patients with intrahepatic cholangiocarcinoma (ICCA), 49 patients with extrahepatic cholangiocarcinoma (ECCA) and 40 patients with GBC were followed up in Eastern Hepatobiliary Hospital for new generation sequencing (NGS). And we also followed up 25 cases of cholangiocarcinoma with PD1 inhibitor and observed the therapeutic effect. Result: The result show that TP53, KRAS, ARID1A, CDKN2A, SMAD4 and ERBB are the most common mutations in ICCA. TP53, KRAS, CDKN2A, ARID2, SMAD4 and ARID1A are the most common mutations in ECCA. TP53, ERBB，CDKN2A, ARID1A, CCNE1 are the most common mutations in GBC. 25 patients who used PD1 inhibitors had no complete remission, 6 patients had partial remission, 6 patients had no effect at all, and the disease progressed. The objective remission rate reached 24% and progression free survival was 5.2±4.8 months. Conclusion: TP53, ARID1A, CDKN2A are the most common mutations in CCA or GBC. We believe that through continuous clinical trials, we will find drugs to inhibit these mutations. PD-1 inhibitor can be used as a choice for patients with advanced stage of CCA or GBC, but not all of them have good therapeutic effect. It is suggested to combine other methods for treatment.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)
DOI	10.11648/j.ijcems.20210704.14
Page(s)	91-97
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Cholangiocarcinoma, Gallbladder Cancer, New Generation Gene Sequencing, PD-1 Inhibitor

References

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APA Style

Hao Song, Hongyan Ma, Yao Huang, Bin Yi, Xiaoqing Jiang. (2021). New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. International Journal of Clinical and Experimental Medical Sciences, 7(4), 91-97. https://doi.org/10.11648/j.ijcems.20210704.14

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ACS Style

Hao Song; Hongyan Ma; Yao Huang; Bin Yi; Xiaoqing Jiang. New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 91-97. doi: 10.11648/j.ijcems.20210704.14

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AMA Style

Hao Song, Hongyan Ma, Yao Huang, Bin Yi, Xiaoqing Jiang. New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. Int J Clin Exp Med Sci. 2021;7(4):91-97. doi: 10.11648/j.ijcems.20210704.14

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@article{10.11648/j.ijcems.20210704.14,
  author = {Hao Song and Hongyan Ma and Yao Huang and Bin Yi and Xiaoqing Jiang},
  title = {New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {91-97},
  doi = {10.11648/j.ijcems.20210704.14},
  url = {https://doi.org/10.11648/j.ijcems.20210704.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.14},
  abstract = {Objective: To explore the common mutation gene and mutation rule of cholangiocarcinoma and gallbladder cancer, and to confirm the therapeutic effect of PD1 inhibitor through follow-up. Method: From 2017 to 2019, 42 patients with intrahepatic cholangiocarcinoma (ICCA), 49 patients with extrahepatic cholangiocarcinoma (ECCA) and 40 patients with GBC were followed up in Eastern Hepatobiliary Hospital for new generation sequencing (NGS). And we also followed up 25 cases of cholangiocarcinoma with PD1 inhibitor and observed the therapeutic effect. Result: The result show that TP53, KRAS, ARID1A, CDKN2A, SMAD4 and ERBB are the most common mutations in ICCA. TP53, KRAS, CDKN2A, ARID2, SMAD4 and ARID1A are the most common mutations in ECCA. TP53, ERBB，CDKN2A, ARID1A, CCNE1 are the most common mutations in GBC. 25 patients who used PD1 inhibitors had no complete remission, 6 patients had partial remission, 6 patients had no effect at all, and the disease progressed. The objective remission rate reached 24% and progression free survival was 5.2±4.8 months. Conclusion: TP53, ARID1A, CDKN2A are the most common mutations in CCA or GBC. We believe that through continuous clinical trials, we will find drugs to inhibit these mutations. PD-1 inhibitor can be used as a choice for patients with advanced stage of CCA or GBC, but not all of them have good therapeutic effect. It is suggested to combine other methods for treatment.},
 year = {2021}
}

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TY - JOUR
T1 - New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy
AU - Hao Song
AU - Hongyan Ma
AU - Yao Huang
AU - Bin Yi
AU - Xiaoqing Jiang
Y1 - 2021/07/16
PY - 2021
N1 - https://doi.org/10.11648/j.ijcems.20210704.14
DO - 10.11648/j.ijcems.20210704.14
T2 - International Journal of Clinical and Experimental Medical Sciences
JF - International Journal of Clinical and Experimental Medical Sciences
JO - International Journal of Clinical and Experimental Medical Sciences
SP - 91
EP - 97
PB - Science Publishing Group
SN - 2469-8032
UR - https://doi.org/10.11648/j.ijcems.20210704.14
AB - Objective: To explore the common mutation gene and mutation rule of cholangiocarcinoma and gallbladder cancer, and to confirm the therapeutic effect of PD1 inhibitor through follow-up. Method: From 2017 to 2019, 42 patients with intrahepatic cholangiocarcinoma (ICCA), 49 patients with extrahepatic cholangiocarcinoma (ECCA) and 40 patients with GBC were followed up in Eastern Hepatobiliary Hospital for new generation sequencing (NGS). And we also followed up 25 cases of cholangiocarcinoma with PD1 inhibitor and observed the therapeutic effect. Result: The result show that TP53, KRAS, ARID1A, CDKN2A, SMAD4 and ERBB are the most common mutations in ICCA. TP53, KRAS, CDKN2A, ARID2, SMAD4 and ARID1A are the most common mutations in ECCA. TP53, ERBB，CDKN2A, ARID1A, CCNE1 are the most common mutations in GBC. 25 patients who used PD1 inhibitors had no complete remission, 6 patients had partial remission, 6 patients had no effect at all, and the disease progressed. The objective remission rate reached 24% and progression free survival was 5.2±4.8 months. Conclusion: TP53, ARID1A, CDKN2A are the most common mutations in CCA or GBC. We believe that through continuous clinical trials, we will find drugs to inhibit these mutations. PD-1 inhibitor can be used as a choice for patients with advanced stage of CCA or GBC, but not all of them have good therapeutic effect. It is suggested to combine other methods for treatment.
VL - 7
IS - 4
ER -

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Author Information

Hao Song

Department of Organ Transplantation, Eastern Hepatobiliary Surgery Hospital Affiliated to Shanghai Second Military Medical University, Shanghai, China
Hongyan Ma

Pharmacy Department, Shenzhen Longhua District Central Hospital, Shenzhen, China
Yao Huang

Department of Organ Transplantation, Eastern Hepatobiliary Surgery Hospital Affiliated to Shanghai Second Military Medical University, Shanghai, China
Bin Yi

Department of Organ Transplantation, Eastern Hepatobiliary Surgery Hospital Affiliated to Shanghai Second Military Medical University, Shanghai, China
Xiaoqing Jiang

Department of Organ Transplantation, Eastern Hepatobiliary Surgery Hospital Affiliated to Shanghai Second Military Medical University, Shanghai, China

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APA Style

Hao Song, Hongyan Ma, Yao Huang, Bin Yi, Xiaoqing Jiang. (2021). New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. International Journal of Clinical and Experimental Medical Sciences, 7(4), 91-97. https://doi.org/10.11648/j.ijcems.20210704.14

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ACS Style

Hao Song; Hongyan Ma; Yao Huang; Bin Yi; Xiaoqing Jiang. New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 91-97. doi: 10.11648/j.ijcems.20210704.14

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AMA Style

Hao Song, Hongyan Ma, Yao Huang, Bin Yi, Xiaoqing Jiang. New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy. Int J Clin Exp Med Sci. 2021;7(4):91-97. doi: 10.11648/j.ijcems.20210704.14

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@article{10.11648/j.ijcems.20210704.14,
  author = {Hao Song and Hongyan Ma and Yao Huang and Bin Yi and Xiaoqing Jiang},
  title = {New Generation Sequencing in Cholangiocarcinoma and Gallbladder Cancer: Clinical Implications for Immunotherapy},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {91-97},
  doi = {10.11648/j.ijcems.20210704.14},
  url = {https://doi.org/10.11648/j.ijcems.20210704.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.14},
  abstract = {Objective: To explore the common mutation gene and mutation rule of cholangiocarcinoma and gallbladder cancer, and to confirm the therapeutic effect of PD1 inhibitor through follow-up. Method: From 2017 to 2019, 42 patients with intrahepatic cholangiocarcinoma (ICCA), 49 patients with extrahepatic cholangiocarcinoma (ECCA) and 40 patients with GBC were followed up in Eastern Hepatobiliary Hospital for new generation sequencing (NGS). And we also followed up 25 cases of cholangiocarcinoma with PD1 inhibitor and observed the therapeutic effect. Result: The result show that TP53, KRAS, ARID1A, CDKN2A, SMAD4 and ERBB are the most common mutations in ICCA. TP53, KRAS, CDKN2A, ARID2, SMAD4 and ARID1A are the most common mutations in ECCA. TP53, ERBB，CDKN2A, ARID1A, CCNE1 are the most common mutations in GBC. 25 patients who used PD1 inhibitors had no complete remission, 6 patients had partial remission, 6 patients had no effect at all, and the disease progressed. The objective remission rate reached 24% and progression free survival was 5.2±4.8 months. Conclusion: TP53, ARID1A, CDKN2A are the most common mutations in CCA or GBC. We believe that through continuous clinical trials, we will find drugs to inhibit these mutations. PD-1 inhibitor can be used as a choice for patients with advanced stage of CCA or GBC, but not all of them have good therapeutic effect. It is suggested to combine other methods for treatment.},
 year = {2021}
}

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