Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in <i>db/db</i> Mice

Xinting Wang; Jihong Wu; Simin Qi; Rui Huang; Xiaoping Wu; Lin Tian; Peili Wang; Shenglan Wang

doi:doi:10.11648/j.ijde.20210604.15

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Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice

Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, Lin Tian, Peili Wang, Shenglan Wang

Published in International Journal of Diabetes and Endocrinology (Volume 6, Issue 4)

Received: 27 October 2021 Accepted: 22 November 2021 Published: 2 December 2021

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Abstract

Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.

Published in	International Journal of Diabetes and Endocrinology (Volume 6, Issue 4)
DOI	10.11648/j.ijde.20210604.15
Page(s)	150-159
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Gut Microbiota, Painful Diabetic Neuropathy, Mechanical Allodynia, Metformin

References

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APA Style

Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. (2021). Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. International Journal of Diabetes and Endocrinology, 6(4), 150-159. https://doi.org/10.11648/j.ijde.20210604.15

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ACS Style

Xinting Wang; Jihong Wu; Simin Qi; Rui Huang; Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int. J. Diabetes Endocrinol. 2021, 6(4), 150-159. doi: 10.11648/j.ijde.20210604.15

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AMA Style

Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int J Diabetes Endocrinol. 2021;6(4):150-159. doi: 10.11648/j.ijde.20210604.15

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@article{10.11648/j.ijde.20210604.15,
  author = {Xinting Wang and Jihong Wu and Simin Qi and Rui Huang and Xiaoping Wu and Lin Tian and Peili Wang and Shenglan Wang},
  title = {Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice},
  journal = {International Journal of Diabetes and Endocrinology},
  volume = {6},
  number = {4},
  pages = {150-159},
  doi = {10.11648/j.ijde.20210604.15},
  url = {https://doi.org/10.11648/j.ijde.20210604.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20210604.15},
  abstract = {Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.},
 year = {2021}
}

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TY  - JOUR
T1  - Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice
AU  - Xinting Wang
AU  - Jihong Wu
AU  - Simin Qi
AU  - Rui Huang
AU  - Xiaoping Wu
AU  - Lin Tian
AU  - Peili Wang
AU  - Shenglan Wang
Y1  - 2021/12/02
PY  - 2021
N1  - https://doi.org/10.11648/j.ijde.20210604.15
DO  - 10.11648/j.ijde.20210604.15
T2  - International Journal of Diabetes and Endocrinology
JF  - International Journal of Diabetes and Endocrinology
JO  - International Journal of Diabetes and Endocrinology
SP  - 150
EP  - 159
PB  - Science Publishing Group
SN  - 2640-1371
UR  - https://doi.org/10.11648/j.ijde.20210604.15
AB  - Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.
VL  - 6
IS  - 4
ER  -

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Author Information

Xinting Wang

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China
Jihong Wu

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China
Simin Qi

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China
Rui Huang

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China
Xiaoping Wu

National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China
Lin Tian

Department of Gerontology, Guang'anmen Hospital, China Academy of Chinese Medical Science, Beijing, China
Peili Wang

National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China
Shenglan Wang

School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

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Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. (2021). Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. International Journal of Diabetes and Endocrinology, 6(4), 150-159. https://doi.org/10.11648/j.ijde.20210604.15

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ACS Style

Xinting Wang; Jihong Wu; Simin Qi; Rui Huang; Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int. J. Diabetes Endocrinol. 2021, 6(4), 150-159. doi: 10.11648/j.ijde.20210604.15

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AMA Style

Xinting Wang, Jihong Wu, Simin Qi, Rui Huang, Xiaoping Wu, et al. Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice. Int J Diabetes Endocrinol. 2021;6(4):150-159. doi: 10.11648/j.ijde.20210604.15

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@article{10.11648/j.ijde.20210604.15,
  author = {Xinting Wang and Jihong Wu and Simin Qi and Rui Huang and Xiaoping Wu and Lin Tian and Peili Wang and Shenglan Wang},
  title = {Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice},
  journal = {International Journal of Diabetes and Endocrinology},
  volume = {6},
  number = {4},
  pages = {150-159},
  doi = {10.11648/j.ijde.20210604.15},
  url = {https://doi.org/10.11648/j.ijde.20210604.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20210604.15},
  abstract = {Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Early-stage Hyperglycemia-induced Gut Microbial Changes Are Partially Associated with Mechanical Allodynia in db/db Mice
AU  - Xinting Wang
AU  - Jihong Wu
AU  - Simin Qi
AU  - Rui Huang
AU  - Xiaoping Wu
AU  - Lin Tian
AU  - Peili Wang
AU  - Shenglan Wang
Y1  - 2021/12/02
PY  - 2021
N1  - https://doi.org/10.11648/j.ijde.20210604.15
DO  - 10.11648/j.ijde.20210604.15
T2  - International Journal of Diabetes and Endocrinology
JF  - International Journal of Diabetes and Endocrinology
JO  - International Journal of Diabetes and Endocrinology
SP  - 150
EP  - 159
PB  - Science Publishing Group
SN  - 2640-1371
UR  - https://doi.org/10.11648/j.ijde.20210604.15
AB  - Recent studies have demonstrated that pain is partially regulated by the gut microbiota. However, the association of gut microbiota with painful diabetic neuropathy, a common complication of diabetes, remains unclear. Herein, we investigated whether the gut microbiota is associated with mechanical allodynia during the early stage of hyperglycemia in 7-week-old db/db mice. The db/db mice were intraperitoneally injected with metformin for 2 weeks. Using the von Frey test and gut microbiota analyses, we investigated the association of gut microbial changes with mechanical allodynia in 7-week-old mice. In db/db mice, colonic microbial community profiles were altered, and both unweighted and weighted UniFrac distances were reduced. Colonic genus-level abundances of Alloprevotella and Prevotellaceae_UCG-001 were positively correlated with mechanical allodynia in db/db mice, while the abundance of Odoribacter was negatively correlated. Intraperitoneal injection of metformin for 2 weeks alleviated mechanical allodynia in db/db mice but did not achieve an anti-diabetic effect. Metformin altered colonic microbial communities and increased weighted UniFrac distance in db/db mice, although its analgesic effect was not associated with specific bacteria. Additionally, alteration of small intestinal microbial community profiles and reduction in weighted UniFrac distance were observed in db/db mice, which were not affected by metformin. These results provide potential evidence of the association of the gut microbiota with mechanical allodynia during early-stage of hyperglycemia.
VL  - 6
IS  - 4
ER  -

Copy | Download