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Effectivity Test on Bacillus thuringiensis Isolate in Land Around Lampung University, Bandar Lampung, Indonesia

Received: 30 January 2017     Accepted: 16 February 2017     Published: 2 March 2017
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Abstract

Until now, efforts to control mosquitoes as vectors still using chemical insecticides. The use of chemicals in continuing can cause resistance towards mosquitoes and cause environmental damage. One of the alternatives that can be utilized is a biological control, such as Bacillus thuringiensis. This research was conducted for three months in the Laboratory of Microbiology Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University. The results showed that isolates of B. thuringiensis from the ground under trees at Lampung University environment equally effective towards Aedes aegypti larval mortality. The study design was Completely Randomized Design (CRD) arranged as factorial. The density of B. thuringiensis isolates 2x107cell / ml Br (A) can cause mortality to larvae of Aedes aegypti high of 22.5% followed by the density of the density of 2x106 cells / ml by 7.5%.

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)
DOI 10.11648/j.ijmb.20170203.11
Page(s) 113-115
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Bacillus thuringiensis, Aedes Aegypti, Mortality

References
[1] Lampung Provincial Health Office, 2015 Years Report.
[2] Becker, N., & J. Margalit, 2003. Use of B. thuringiensis israelensis Against Mosquitoes and Blackflies In: Entwistle, P. F., Corry, J. S., Bailey, M. J., & Higgs, S. Bacillus thuringiensis an Environmental Biopesticide: Theory and Practice. John Willey & Sons Chichester. Inggris.
[3] Salaki, C. L., dan Sembiring, L. 2009,. Eksplorasi Bakteri B. thuringiensis dari Berbagai Habitat Alami yang Berpotensi sebagai Agensia Pengendali Hayati Nyamuk Aedes aegypti Linnaeus. Prosiding Biteknologi. Seminar Nasional Biologi XX dan Kongres PBI XIV UIN Maliki Malang.
[4] Pakpahan, M. 2013. Karakterisasi Fisiologi dan Pertumbuhan B. thuringiensis dari Tanah Naungan di Lingkungan Universitas Lampung. Seminar Nasional Sains & Teknologi V Lembaga Penelitian Universitas Lampung. Bandar Lampung.
[5] Widiyanti, P. M. N. dan Muyadihardja, Sanusi. 2004. Uji Toksisitas Jamur Metarhizium anisopliae terhadap larva Nyamuk Aedes aegypti. Media Litbang Kesehatan, Volume XIV Nomor 3. Hal 25-30.
[6] Rasko DA, M. R. Altherr, Han CS, J. Ravel, 2005 Genomics of the Bacillus cereus group of organisms. FEMS Microbiol Rev 29 (2): 303–329.
[7] Salaki, C. L., Situmorang, J., dan L. Sembiring, 2009, Isolation and Characterization of Indonesian Indigenous Bacteria (Bacillu thuringiensis) which are Potential for Biological Control Agent Against Cabbage Heart Caterpillar (Crocidolomia binotalis Zell). Jurnal Eugennia, hal. 1-6. 39.
[8] Swadener C. 1994. B. thuringiensis Journal Pesticed Reform 14 (3): 14-20. Northwest Coalition for Alternatif ti Pesticides. Canada Environment Microbiological.62: 80–6.
[9] Sukarno, Blondine Ch. P. R., Wiranto. 2000. Pengendalian Vektor (Jentik) Demam Berdarah, Malaria, Filariasis menggunakan Strain Lokal B. thuringiensis Varietas Israelensis. Medika. 26 (1): 16-19.
[10] Rao MB, Tanksale A. M, Ghatge MS, V. V. Deshpande, 1998. Molecular and Biotechnological aspect of microbial proteases. Microbiology Molecular Biology Review 62: 597-635.
[11] Agaisse. H and D. Lereclus, 1995. B. thuringiensis produce so much insecticidal crystal protein. Journal Bacteriological. 21: 6027–32.
[12] Lambert B, Buysse L, Decock C, Jansens S, Piens C, Saey B, Seurinck J, Van Audenhove K, Van Rie J, Van Vliet A dan Peferoen M. 1996. A B. thuringiensis Insecticidal Crystal Protein with a High Activity against Members of the Family Noctuidae. Applied.
[13] Trizelia,2001. Makalah Pemanfaatan B. thuringiensis untuk pengendalian Hama Crocidolomia binotalis. Institut Pertanian Bogor.
[14] Gama, Z. P., B. Yanuwiadi, and T. H. Kurniati, 2010. Strategi Pemberantasan Nyamuk Aman Lingkungan: Potensi B. thuringiensis Isolat Madura Sebagai Musuh Alami Nyamuk Aedes aegypti. Jurnal Pembangunan dan Alam Lestari. 1 (1): 2087–3522.
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  • APA Style

    Emantis Rosa, Christina Nugroho Ekowati, Ambar Prameswari. (2017). Effectivity Test on Bacillus thuringiensis Isolate in Land Around Lampung University, Bandar Lampung, Indonesia. International Journal of Microbiology and Biotechnology, 2(3), 113-115. https://doi.org/10.11648/j.ijmb.20170203.11

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    ACS Style

    Emantis Rosa; Christina Nugroho Ekowati; Ambar Prameswari. Effectivity Test on Bacillus thuringiensis Isolate in Land Around Lampung University, Bandar Lampung, Indonesia. Int. J. Microbiol. Biotechnol. 2017, 2(3), 113-115. doi: 10.11648/j.ijmb.20170203.11

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    AMA Style

    Emantis Rosa, Christina Nugroho Ekowati, Ambar Prameswari. Effectivity Test on Bacillus thuringiensis Isolate in Land Around Lampung University, Bandar Lampung, Indonesia. Int J Microbiol Biotechnol. 2017;2(3):113-115. doi: 10.11648/j.ijmb.20170203.11

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  • @article{10.11648/j.ijmb.20170203.11,
      author = {Emantis Rosa and Christina Nugroho Ekowati and Ambar Prameswari},
      title = {Effectivity Test on Bacillus thuringiensis Isolate in Land Around Lampung University, Bandar Lampung, Indonesia},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {3},
      pages = {113-115},
      doi = {10.11648/j.ijmb.20170203.11},
      url = {https://doi.org/10.11648/j.ijmb.20170203.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.11},
      abstract = {Until now, efforts to control mosquitoes as vectors still using chemical insecticides. The use of chemicals in continuing can cause resistance towards mosquitoes and cause environmental damage. One of the alternatives that can be utilized is a biological control, such as Bacillus thuringiensis. This research was conducted for three months in the Laboratory of Microbiology Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University. The results showed that isolates of B. thuringiensis from the ground under trees at Lampung University environment equally effective towards Aedes aegypti larval mortality. The study design was Completely Randomized Design (CRD) arranged as factorial. The density of B. thuringiensis isolates 2x107cell / ml Br (A) can cause mortality to larvae of Aedes aegypti high of 22.5% followed by the density of the density of 2x106 cells / ml by 7.5%.},
     year = {2017}
    }
    

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    AB  - Until now, efforts to control mosquitoes as vectors still using chemical insecticides. The use of chemicals in continuing can cause resistance towards mosquitoes and cause environmental damage. One of the alternatives that can be utilized is a biological control, such as Bacillus thuringiensis. This research was conducted for three months in the Laboratory of Microbiology Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University. The results showed that isolates of B. thuringiensis from the ground under trees at Lampung University environment equally effective towards Aedes aegypti larval mortality. The study design was Completely Randomized Design (CRD) arranged as factorial. The density of B. thuringiensis isolates 2x107cell / ml Br (A) can cause mortality to larvae of Aedes aegypti high of 22.5% followed by the density of the density of 2x106 cells / ml by 7.5%.
    VL  - 2
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Author Information
  • Department of Biology, Lampung University, Lampung, Indonesia

  • Department of Biology, Lampung University, Lampung, Indonesia

  • Department of Biology, Lampung University, Lampung, Indonesia

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