Release of petroleum oil and its products into the environment is a worldwide concern. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbon-degrading properties. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Out of twenty one microbes isolated, nine were selected based on their ability to utilize different hydrocarbons. The isolates were observed to mineralize heating oil, hexane, octane, toluene and diesel oil. PCR amplification of 16S rDNA gene revealed that the isolates belong to six different genera; Pseudomonas, Acinetobacter, Klebsiella, Enterobacter, Salmonella and Ochrobactrum. Based on their ability to degrade diesel oil, three isolates were selected and their growth conditions optimized. Optimum degradation was recorded at less than 1% substrate concentration, pH 7 and temperature range of between 30°C and 37°C and using yeast extract as nitrogen source. GC-MS analyses confirmed their diesel oil degrading properties. Alkane hydroxylase gene from one of the isolates (isolate 1C) was successfully amplified indicating its catabolic capabilities in degrading alkanes. Overall, the characterized isolates may constitute potential candidates for biotechnological application in environmental cleanup of petroleum contaminants.
| Published in | International Journal of Microbiology and Biotechnology (Volume 3, Issue 1) |
| DOI | 10.11648/j.ijmb.20180301.13 |
| Page(s) | 11-24 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Oil-Contaminated Soils, Bacteria, Biodegradation, Petroleum Hydrocarbons
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APA Style
Agnes Njoki Mwaura, Betty Nyambura Mbatia, Edward Kirwa Muge, Patrick Wafula Okanya. (2018). Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages. International Journal of Microbiology and Biotechnology, 3(1), 11-24. https://doi.org/10.11648/j.ijmb.20180301.13
ACS Style
Agnes Njoki Mwaura; Betty Nyambura Mbatia; Edward Kirwa Muge; Patrick Wafula Okanya. Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages. Int. J. Microbiol. Biotechnol. 2018, 3(1), 11-24. doi: 10.11648/j.ijmb.20180301.13
AMA Style
Agnes Njoki Mwaura, Betty Nyambura Mbatia, Edward Kirwa Muge, Patrick Wafula Okanya. Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages. Int J Microbiol Biotechnol. 2018;3(1):11-24. doi: 10.11648/j.ijmb.20180301.13
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Download@article{10.11648/j.ijmb.20180301.13,
author = {Agnes Njoki Mwaura and Betty Nyambura Mbatia and Edward Kirwa Muge and Patrick Wafula Okanya},
title = {Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages},
journal = {International Journal of Microbiology and Biotechnology},
volume = {3},
number = {1},
pages = {11-24},
doi = {10.11648/j.ijmb.20180301.13},
url = {https://doi.org/10.11648/j.ijmb.20180301.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180301.13},
abstract = {Release of petroleum oil and its products into the environment is a worldwide concern. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbon-degrading properties. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Out of twenty one microbes isolated, nine were selected based on their ability to utilize different hydrocarbons. The isolates were observed to mineralize heating oil, hexane, octane, toluene and diesel oil. PCR amplification of 16S rDNA gene revealed that the isolates belong to six different genera; Pseudomonas, Acinetobacter, Klebsiella, Enterobacter, Salmonella and Ochrobactrum. Based on their ability to degrade diesel oil, three isolates were selected and their growth conditions optimized. Optimum degradation was recorded at less than 1% substrate concentration, pH 7 and temperature range of between 30°C and 37°C and using yeast extract as nitrogen source. GC-MS analyses confirmed their diesel oil degrading properties. Alkane hydroxylase gene from one of the isolates (isolate 1C) was successfully amplified indicating its catabolic capabilities in degrading alkanes. Overall, the characterized isolates may constitute potential candidates for biotechnological application in environmental cleanup of petroleum contaminants.},
year = {2018}
}
TY - JOUR T1 - Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages AU - Agnes Njoki Mwaura AU - Betty Nyambura Mbatia AU - Edward Kirwa Muge AU - Patrick Wafula Okanya Y1 - 2018/03/23 PY - 2018 N1 - https://doi.org/10.11648/j.ijmb.20180301.13 DO - 10.11648/j.ijmb.20180301.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 11 EP - 24 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20180301.13 AB - Release of petroleum oil and its products into the environment is a worldwide concern. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbon-degrading properties. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Out of twenty one microbes isolated, nine were selected based on their ability to utilize different hydrocarbons. The isolates were observed to mineralize heating oil, hexane, octane, toluene and diesel oil. PCR amplification of 16S rDNA gene revealed that the isolates belong to six different genera; Pseudomonas, Acinetobacter, Klebsiella, Enterobacter, Salmonella and Ochrobactrum. Based on their ability to degrade diesel oil, three isolates were selected and their growth conditions optimized. Optimum degradation was recorded at less than 1% substrate concentration, pH 7 and temperature range of between 30°C and 37°C and using yeast extract as nitrogen source. GC-MS analyses confirmed their diesel oil degrading properties. Alkane hydroxylase gene from one of the isolates (isolate 1C) was successfully amplified indicating its catabolic capabilities in degrading alkanes. Overall, the characterized isolates may constitute potential candidates for biotechnological application in environmental cleanup of petroleum contaminants. VL - 3 IS - 1 ER -
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