With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.
| Published in | International Journal of Microbiology and Biotechnology (Volume 3, Issue 1) |
| DOI | 10.11648/j.ijmb.20180301.14 |
| Page(s) | 25-30 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Actinomycetes, Chitwan, Antibacterial
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APA Style
Kamal Rai, Sujan Khadka, Bidya Shrestha. (2018). Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. International Journal of Microbiology and Biotechnology, 3(1), 25-30. https://doi.org/10.11648/j.ijmb.20180301.14
ACS Style
Kamal Rai; Sujan Khadka; Bidya Shrestha. Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. Int. J. Microbiol. Biotechnol. 2018, 3(1), 25-30. doi: 10.11648/j.ijmb.20180301.14
AMA Style
Kamal Rai, Sujan Khadka, Bidya Shrestha. Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal. Int J Microbiol Biotechnol. 2018;3(1):25-30. doi: 10.11648/j.ijmb.20180301.14
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Download@article{10.11648/j.ijmb.20180301.14,
author = {Kamal Rai and Sujan Khadka and Bidya Shrestha},
title = {Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal},
journal = {International Journal of Microbiology and Biotechnology},
volume = {3},
number = {1},
pages = {25-30},
doi = {10.11648/j.ijmb.20180301.14},
url = {https://doi.org/10.11648/j.ijmb.20180301.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180301.14},
abstract = {With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.},
year = {2018}
}
TY - JOUR T1 - Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal AU - Kamal Rai AU - Sujan Khadka AU - Bidya Shrestha Y1 - 2018/04/10 PY - 2018 N1 - https://doi.org/10.11648/j.ijmb.20180301.14 DO - 10.11648/j.ijmb.20180301.14 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 25 EP - 30 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20180301.14 AB - With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production. VL - 3 IS - 1 ER -
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