Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar

Hanitrinisoa Harimisa Andriamafana; Yves Mong; Onja Andriambeloson; Christine Ravonizafy; Marson Raherimandimby; Rado Rasolomampianina

doi:doi:10.11648/j.ijmb.20180303.12

Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar

Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, Rado Rasolomampianina

Published in International Journal of Microbiology and Biotechnology (Volume 3, Issue 3)

Received: 11 September 2018 Accepted: 21 September 2018 Published: 30 October 2018

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Abstract

Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.

Published in	International Journal of Microbiology and Biotechnology (Volume 3, Issue 3)
DOI	10.11648/j.ijmb.20180303.12
Page(s)	71-78
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Isolation, Characterization, Resistance, Heavy Metals, Antibiotics, Dumpsite, MADAGASCAR

References

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Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. (2018). Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. International Journal of Microbiology and Biotechnology, 3(3), 71-78. https://doi.org/10.11648/j.ijmb.20180303.12

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Hanitrinisoa Harimisa Andriamafana; Yves Mong; Onja Andriambeloson; Christine Ravonizafy; Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int. J. Microbiol. Biotechnol. 2018, 3(3), 71-78. doi: 10.11648/j.ijmb.20180303.12

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Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int J Microbiol Biotechnol. 2018;3(3):71-78. doi: 10.11648/j.ijmb.20180303.12

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@article{10.11648/j.ijmb.20180303.12,
  author = {Hanitrinisoa Harimisa Andriamafana and Yves Mong and Onja Andriambeloson and Christine Ravonizafy and Marson Raherimandimby and Rado Rasolomampianina},
  title = {Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {3},
  number = {3},
  pages = {71-78},
  doi = {10.11648/j.ijmb.20180303.12},
  url = {https://doi.org/10.11648/j.ijmb.20180303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180303.12},
  abstract = {Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.},
 year = {2018}
}

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TY  - JOUR
T1  - Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar
AU  - Hanitrinisoa Harimisa Andriamafana
AU  - Yves Mong
AU  - Onja Andriambeloson
AU  - Christine Ravonizafy
AU  - Marson Raherimandimby
AU  - Rado Rasolomampianina
Y1  - 2018/10/30
PY  - 2018
N1  - https://doi.org/10.11648/j.ijmb.20180303.12
DO  - 10.11648/j.ijmb.20180303.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 71
EP  - 78
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20180303.12
AB  - Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.
VL  - 3
IS  - 3
ER  -

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Author Information

Hanitrinisoa Harimisa Andriamafana

Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Yves Mong

National Center of Environmental Research, Antananarivo, Madagascar
Onja Andriambeloson

National Center of Environmental Research, Antananarivo, Madagascar
Christine Ravonizafy

National Center of Environmental Research, Antananarivo, Madagascar
Marson Raherimandimby

Department of Fundamental and Applied Biochemistry, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Rado Rasolomampianina

National Center of Environmental Research, Antananarivo, Madagascar

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APA Style

Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. (2018). Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. International Journal of Microbiology and Biotechnology, 3(3), 71-78. https://doi.org/10.11648/j.ijmb.20180303.12

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Hanitrinisoa Harimisa Andriamafana; Yves Mong; Onja Andriambeloson; Christine Ravonizafy; Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int. J. Microbiol. Biotechnol. 2018, 3(3), 71-78. doi: 10.11648/j.ijmb.20180303.12

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AMA Style

Hanitrinisoa Harimisa Andriamafana, Yves Mong, Onja Andriambeloson, Christine Ravonizafy, Marson Raherimandimby, et al. Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar. Int J Microbiol Biotechnol. 2018;3(3):71-78. doi: 10.11648/j.ijmb.20180303.12

Copy | Download

@article{10.11648/j.ijmb.20180303.12,
  author = {Hanitrinisoa Harimisa Andriamafana and Yves Mong and Onja Andriambeloson and Christine Ravonizafy and Marson Raherimandimby and Rado Rasolomampianina},
  title = {Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {3},
  number = {3},
  pages = {71-78},
  doi = {10.11648/j.ijmb.20180303.12},
  url = {https://doi.org/10.11648/j.ijmb.20180303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180303.12},
  abstract = {Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Identification of Heavy Metals and Antibiotics Resistant Strains from Antananarivo Dumpsite, Madagascar
AU  - Hanitrinisoa Harimisa Andriamafana
AU  - Yves Mong
AU  - Onja Andriambeloson
AU  - Christine Ravonizafy
AU  - Marson Raherimandimby
AU  - Rado Rasolomampianina
Y1  - 2018/10/30
PY  - 2018
N1  - https://doi.org/10.11648/j.ijmb.20180303.12
DO  - 10.11648/j.ijmb.20180303.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 71
EP  - 78
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20180303.12
AB  - Heavy metals contamination is now widespread in the nature. At higher concentration, heavy metals become toxic and disturb the ecosystem including soil microorganisms. To adapt to such constraints, some microorganisms have developed tolerance mechanisms. Indeed, in the environment, the resistance of microorganisms to heavy metal often promotes to antibiotic resistance. This work aims to isolate strains from soil samples collected in Andralanitra landfill, to test their tolerance to heavy metals, to identify tolerant strains and to verify their resistance to antibiotics. According to the dilution method, a total of 48 strains were obtained, 14 were isolated on PDA medium, 10 on Sabouraud agar medium, 10 strains on Mossel agar medium, 7 on AS1 medium, 5 strains on TSA medium and 2 strains with King B medium. Resistance test to heavy metals performed by the wells method showed that out of the 48 isolated strains, 26 were capable to grow in the presence of heavy metals (solution composed of copper, zinc, cadmium, chromium, nickel, lead) at different concentrations. The highest number of tolerant strains was recorded at the concentration of 100mg/L ≤ C ≤ 1000mg/L. Four (4) strains were tolerant to the heavy metals solution at a concentration between 100mg/L and 1500mg/L. The molecular identification of these four most resistant strains by 16S rDNA gene sequencing and ITS gene sequencing allowed to classify them as belonging to the genera Ochrobactrum pseudogrignonense, Arthrobacter nicotianae, Penicillium crustosum and Penicillium commune. The antibiotic sensitivity test using disc diffusion method on Mueller-Hinton agar revealed that Ochrobactrum pseudogrignonense and Penicillium commune were resistant to Trimethoprim, Arthrobacter nicotianae showed resistance to Trimethoprim and Ciprofloxacin, Penicillium crustosum was resistant to all tested antibiotics.
VL  - 3
IS  - 3
ER  -

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