Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp

Bryan Pillacela; Janeth Galarza; Carolina Tufiño

doi:doi:10.11648/j.ijmb.20200501.13

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Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp

Bryan Pillacela, Janeth Galarza, Carolina Tufiño

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 1)

Received: 22 December 2019 Accepted: 17 January 2020 Published: 31 January 2020

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Abstract

Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.

Published in	International Journal of Microbiology and Biotechnology (Volume 5, Issue 1)
DOI	10.11648/j.ijmb.20200501.13
Page(s)	16-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

In silico Model, Phytoene Desaturase, Restriction Enzymes, Bioinformatics, Haematococcus sp

References

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APA Style

Bryan Pillacela, Janeth Galarza, Carolina Tufiño. (2020). Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. International Journal of Microbiology and Biotechnology, 5(1), 16-21. https://doi.org/10.11648/j.ijmb.20200501.13

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ACS Style

Bryan Pillacela; Janeth Galarza; Carolina Tufiño. Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. Int. J. Microbiol. Biotechnol. 2020, 5(1), 16-21. doi: 10.11648/j.ijmb.20200501.13

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AMA Style

Bryan Pillacela, Janeth Galarza, Carolina Tufiño. Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. Int J Microbiol Biotechnol. 2020;5(1):16-21. doi: 10.11648/j.ijmb.20200501.13

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@article{10.11648/j.ijmb.20200501.13,
  author = {Bryan Pillacela and Janeth Galarza and Carolina Tufiño},
  title = {Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {1},
  pages = {16-21},
  doi = {10.11648/j.ijmb.20200501.13},
  url = {https://doi.org/10.11648/j.ijmb.20200501.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.13},
  abstract = {Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.},
 year = {2020}
}

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TY  - JOUR
T1  - Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp
AU  - Bryan Pillacela
AU  - Janeth Galarza
AU  - Carolina Tufiño
Y1  - 2020/01/31
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200501.13
DO  - 10.11648/j.ijmb.20200501.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 16
EP  - 21
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200501.13
AB  - Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.
VL  - 5
IS  - 1
ER  -

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Author Information

Bryan Pillacela

Biology Research Center, Faculty of Marine Sciences, Santa Elena Peninsula State University, La Libertad, Ecuador
Janeth Galarza

Biology Research Center, Faculty of Marine Sciences, Santa Elena Peninsula State University, La Libertad, Ecuador
Carolina Tufiño

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APA Style

Bryan Pillacela, Janeth Galarza, Carolina Tufiño. (2020). Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. International Journal of Microbiology and Biotechnology, 5(1), 16-21. https://doi.org/10.11648/j.ijmb.20200501.13

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ACS Style

Bryan Pillacela; Janeth Galarza; Carolina Tufiño. Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. Int. J. Microbiol. Biotechnol. 2020, 5(1), 16-21. doi: 10.11648/j.ijmb.20200501.13

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AMA Style

Bryan Pillacela, Janeth Galarza, Carolina Tufiño. Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp. Int J Microbiol Biotechnol. 2020;5(1):16-21. doi: 10.11648/j.ijmb.20200501.13

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@article{10.11648/j.ijmb.20200501.13,
  author = {Bryan Pillacela and Janeth Galarza and Carolina Tufiño},
  title = {Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {1},
  pages = {16-21},
  doi = {10.11648/j.ijmb.20200501.13},
  url = {https://doi.org/10.11648/j.ijmb.20200501.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.13},
  abstract = {Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Reliability of in silico Modeling Based on Restriction Patterns for the Study of the Carotenogenic Gene pds of Haematococcus sp
AU  - Bryan Pillacela
AU  - Janeth Galarza
AU  - Carolina Tufiño
Y1  - 2020/01/31
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200501.13
DO  - 10.11648/j.ijmb.20200501.13
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 16
EP  - 21
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200501.13
AB  - Phytoeno desaturase (pds gene) is an enzyme involved in the biosynthesis of carotenoids such as β-carotene and astaxanthin in microalgae and some autotrophic organisms. Carotenoids have antioxidant, anti-inflammatory and anti-carcinogenic properties. Prior to biotechnological studies it is necessary to carry out the integral analysis of this carotenogenic gene and its molecules for future industrial applications. In silico modeling is an important bioinformatics tool that allows to adjust, summarize and organize experimental information, test theories and generally understand the interaction of molecules and biological systems. In this work, specific primers were used to amplify the phytoene desaturase (pds) gene of Haematococcus sp. (PM015). NEBcutter V2.0 and BioEdit provided the simulation design of the pds restriction patterns together with the restriction enzymes FsPI, AlwI, HincII, BlpI and MluCI. The amplification product resulted in two fragments one of 1200 bp and one of 1700 bp. The in silico design was reproduced and tested experimentally, in this way the identification of the pds gene of Haematococcus sp. (PM015) and the effectiveness of bioinformatics programs as an important alternative for in silico modeling of molecules and/or biological systems, which mapping predict reliable results, saving time and reducing costs in the experimental stage, in addition to allowing the selection of restriction enzymes with optimal enzymatic activity to digest DNA.
VL  - 5
IS  - 1
ER  -

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