Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods

Gedif Meseret Abebe

doi:doi:10.11648/j.ijmb.20200503.17

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Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods

Gedif Meseret Abebe

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 3)

Received: 17 May 2020 Accepted: 29 May 2020 Published: 4 July 2020

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Abstract

Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on our health and food safety. The study was aimed to detect biofilm formation and antibiotic resistance by Klebsiella oxytoca and Klebsiella pneumoniae from animal origin foods. In this study100 food samples were examined for the presence of Klebsiella oxytoca, Klebsiella pneumoniae, and other Enterobacteriaceae family members. In this study, 19 Klebsiella oxytoca and 5 Klebsiella pneumoniae isolates were isolated from cheese and minced meat samples using standard biochemical tests and identification kit. Biofilm formation in these isolates was detected by using microplate, Congo red agar, and tube adherence methods. Antibiotic susceptibility testing was performed using the disk diffusion method on Muller-Hinton agar. Using a microplate method strong biofilm formation was observed in 16 (84%) and 5 (100%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose respectively. After 24 and 48 hours of incubation on Tryptic Soy Broth without glucose strong biofilm formation was detected in 10 (52.6%) and 2 (40%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae respectively. After 24 hours of incubation on Congo red agar 14 (73.7%) and 3 (60%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were slime factor positive respectively. In tube adherence method 13 (68.4%) and 4 (80%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were seen to have adhered strongly after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose. In general, strong biofilm formation by these strains was seen on Tryptic Soy Broth medium when supplemented with glucose. Among all the Klebsiella oxytoca, the highest rates of susceptibility were seen toward Trimethoprim-Sulfamethoxazole (100%) and Imipenem (94.7%) followed by Chloramphenicol (73.7%) and gentamicin (68.4%). Among 19 Klebsiella oxytoca isolates, the highest rates of resistance were seen in streptomycin (73.7%) and Kanamycin (73.7%) followed by ampicillin (63.2%). The majority of Klebsiella pneumoniae isolates were resistant to Kanamycin (80%) and Streptomycin (80%) followed by Amikacin (60%). On the other hand, 80% of Klebsiella pneumoniae were susceptible to imipenem, chloramphenicol, Trimethoprim-Sulfamethoxazole, and cefotaxime. Generally, majority of Klebsiella oxytoca, and Klebsiella pneumoniae isolates showed strong biofilm production on different growth conditions and majority of the isolates were also resistance for antibiotics. Therefore, biofilm production by these nosocomial bacteria has an implication public health and pave the way for increased resistance of biofilm-associated organisms to antimicrobial agents.

Published in	International Journal of Microbiology and Biotechnology (Volume 5, Issue 3)
DOI	10.11648/j.ijmb.20200503.17
Page(s)	120-130
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Klebsiella oxytoca, Klebsiella pneumoniae, Biofilm, Antibiotic Resistance

References

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Gedif Meseret Abebe. (2020). Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. International Journal of Microbiology and Biotechnology, 5(3), 120-130. https://doi.org/10.11648/j.ijmb.20200503.17

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Gedif Meseret Abebe. Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. Int. J. Microbiol. Biotechnol. 2020, 5(3), 120-130. doi: 10.11648/j.ijmb.20200503.17

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Gedif Meseret Abebe. Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. Int J Microbiol Biotechnol. 2020;5(3):120-130. doi: 10.11648/j.ijmb.20200503.17

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@article{10.11648/j.ijmb.20200503.17,
  author = {Gedif Meseret Abebe},
  title = {Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {3},
  pages = {120-130},
  doi = {10.11648/j.ijmb.20200503.17},
  url = {https://doi.org/10.11648/j.ijmb.20200503.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200503.17},
  abstract = {Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on our health and food safety. The study was aimed to detect biofilm formation and antibiotic resistance by Klebsiella oxytoca and Klebsiella pneumoniae from animal origin foods. In this study100 food samples were examined for the presence of Klebsiella oxytoca, Klebsiella pneumoniae, and other Enterobacteriaceae family members. In this study, 19 Klebsiella oxytoca and 5 Klebsiella pneumoniae isolates were isolated from cheese and minced meat samples using standard biochemical tests and identification kit. Biofilm formation in these isolates was detected by using microplate, Congo red agar, and tube adherence methods. Antibiotic susceptibility testing was performed using the disk diffusion method on Muller-Hinton agar. Using a microplate method strong biofilm formation was observed in 16 (84%) and 5 (100%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose respectively. After 24 and 48 hours of incubation on Tryptic Soy Broth without glucose strong biofilm formation was detected in 10 (52.6%) and 2 (40%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae respectively. After 24 hours of incubation on Congo red agar 14 (73.7%) and 3 (60%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were slime factor positive respectively. In tube adherence method 13 (68.4%) and 4 (80%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were seen to have adhered strongly after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose. In general, strong biofilm formation by these strains was seen on Tryptic Soy Broth medium when supplemented with glucose. Among all the Klebsiella oxytoca, the highest rates of susceptibility were seen toward Trimethoprim-Sulfamethoxazole (100%) and Imipenem (94.7%) followed by Chloramphenicol (73.7%) and gentamicin (68.4%). Among 19 Klebsiella oxytoca isolates, the highest rates of resistance were seen in streptomycin (73.7%) and Kanamycin (73.7%) followed by ampicillin (63.2%). The majority of Klebsiella pneumoniae isolates were resistant to Kanamycin (80%) and Streptomycin (80%) followed by Amikacin (60%). On the other hand, 80% of Klebsiella pneumoniae were susceptible to imipenem, chloramphenicol, Trimethoprim-Sulfamethoxazole, and cefotaxime. Generally, majority of Klebsiella oxytoca, and Klebsiella pneumoniae isolates showed strong biofilm production on different growth conditions and majority of the isolates were also resistance for antibiotics. Therefore, biofilm production by these nosocomial bacteria has an implication public health and pave the way for increased resistance of biofilm-associated organisms to antimicrobial agents.},
 year = {2020}
}

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TY  - JOUR
T1  - Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods
AU  - Gedif Meseret Abebe
Y1  - 2020/07/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200503.17
DO  - 10.11648/j.ijmb.20200503.17
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 120
EP  - 130
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200503.17
AB  - Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on our health and food safety. The study was aimed to detect biofilm formation and antibiotic resistance by Klebsiella oxytoca and Klebsiella pneumoniae from animal origin foods. In this study100 food samples were examined for the presence of Klebsiella oxytoca, Klebsiella pneumoniae, and other Enterobacteriaceae family members. In this study, 19 Klebsiella oxytoca and 5 Klebsiella pneumoniae isolates were isolated from cheese and minced meat samples using standard biochemical tests and identification kit. Biofilm formation in these isolates was detected by using microplate, Congo red agar, and tube adherence methods. Antibiotic susceptibility testing was performed using the disk diffusion method on Muller-Hinton agar. Using a microplate method strong biofilm formation was observed in 16 (84%) and 5 (100%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose respectively. After 24 and 48 hours of incubation on Tryptic Soy Broth without glucose strong biofilm formation was detected in 10 (52.6%) and 2 (40%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae respectively. After 24 hours of incubation on Congo red agar 14 (73.7%) and 3 (60%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were slime factor positive respectively. In tube adherence method 13 (68.4%) and 4 (80%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were seen to have adhered strongly after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose. In general, strong biofilm formation by these strains was seen on Tryptic Soy Broth medium when supplemented with glucose. Among all the Klebsiella oxytoca, the highest rates of susceptibility were seen toward Trimethoprim-Sulfamethoxazole (100%) and Imipenem (94.7%) followed by Chloramphenicol (73.7%) and gentamicin (68.4%). Among 19 Klebsiella oxytoca isolates, the highest rates of resistance were seen in streptomycin (73.7%) and Kanamycin (73.7%) followed by ampicillin (63.2%). The majority of Klebsiella pneumoniae isolates were resistant to Kanamycin (80%) and Streptomycin (80%) followed by Amikacin (60%). On the other hand, 80% of Klebsiella pneumoniae were susceptible to imipenem, chloramphenicol, Trimethoprim-Sulfamethoxazole, and cefotaxime. Generally, majority of Klebsiella oxytoca, and Klebsiella pneumoniae isolates showed strong biofilm production on different growth conditions and majority of the isolates were also resistance for antibiotics. Therefore, biofilm production by these nosocomial bacteria has an implication public health and pave the way for increased resistance of biofilm-associated organisms to antimicrobial agents.
VL  - 5
IS  - 3
ER  -

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Gedif Meseret Abebe

Department of Biology, College of Natural and Computational Science, Wolaita Sodo University, Wolaita Sodo, Ethiopia

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Gedif Meseret Abebe. (2020). Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. International Journal of Microbiology and Biotechnology, 5(3), 120-130. https://doi.org/10.11648/j.ijmb.20200503.17

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Gedif Meseret Abebe. Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. Int. J. Microbiol. Biotechnol. 2020, 5(3), 120-130. doi: 10.11648/j.ijmb.20200503.17

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Gedif Meseret Abebe. Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods. Int J Microbiol Biotechnol. 2020;5(3):120-130. doi: 10.11648/j.ijmb.20200503.17

Copy | Download

@article{10.11648/j.ijmb.20200503.17,
  author = {Gedif Meseret Abebe},
  title = {Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {5},
  number = {3},
  pages = {120-130},
  doi = {10.11648/j.ijmb.20200503.17},
  url = {https://doi.org/10.11648/j.ijmb.20200503.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200503.17},
  abstract = {Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on our health and food safety. The study was aimed to detect biofilm formation and antibiotic resistance by Klebsiella oxytoca and Klebsiella pneumoniae from animal origin foods. In this study100 food samples were examined for the presence of Klebsiella oxytoca, Klebsiella pneumoniae, and other Enterobacteriaceae family members. In this study, 19 Klebsiella oxytoca and 5 Klebsiella pneumoniae isolates were isolated from cheese and minced meat samples using standard biochemical tests and identification kit. Biofilm formation in these isolates was detected by using microplate, Congo red agar, and tube adherence methods. Antibiotic susceptibility testing was performed using the disk diffusion method on Muller-Hinton agar. Using a microplate method strong biofilm formation was observed in 16 (84%) and 5 (100%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose respectively. After 24 and 48 hours of incubation on Tryptic Soy Broth without glucose strong biofilm formation was detected in 10 (52.6%) and 2 (40%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae respectively. After 24 hours of incubation on Congo red agar 14 (73.7%) and 3 (60%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were slime factor positive respectively. In tube adherence method 13 (68.4%) and 4 (80%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were seen to have adhered strongly after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose. In general, strong biofilm formation by these strains was seen on Tryptic Soy Broth medium when supplemented with glucose. Among all the Klebsiella oxytoca, the highest rates of susceptibility were seen toward Trimethoprim-Sulfamethoxazole (100%) and Imipenem (94.7%) followed by Chloramphenicol (73.7%) and gentamicin (68.4%). Among 19 Klebsiella oxytoca isolates, the highest rates of resistance were seen in streptomycin (73.7%) and Kanamycin (73.7%) followed by ampicillin (63.2%). The majority of Klebsiella pneumoniae isolates were resistant to Kanamycin (80%) and Streptomycin (80%) followed by Amikacin (60%). On the other hand, 80% of Klebsiella pneumoniae were susceptible to imipenem, chloramphenicol, Trimethoprim-Sulfamethoxazole, and cefotaxime. Generally, majority of Klebsiella oxytoca, and Klebsiella pneumoniae isolates showed strong biofilm production on different growth conditions and majority of the isolates were also resistance for antibiotics. Therefore, biofilm production by these nosocomial bacteria has an implication public health and pave the way for increased resistance of biofilm-associated organisms to antimicrobial agents.},
 year = {2020}
}

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TY  - JOUR
T1  - Detection of Biofilm Formation and Antibiotic Resistance in Klebsiella Oxytoca and Klebsiella Pneumoniae from Animal Origin Foods
AU  - Gedif Meseret Abebe
Y1  - 2020/07/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ijmb.20200503.17
DO  - 10.11648/j.ijmb.20200503.17
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 120
EP  - 130
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20200503.17
AB  - Biofilms are surface-attached microbial communities with distinct properties, which have a tremendous impact on our health and food safety. The study was aimed to detect biofilm formation and antibiotic resistance by Klebsiella oxytoca and Klebsiella pneumoniae from animal origin foods. In this study100 food samples were examined for the presence of Klebsiella oxytoca, Klebsiella pneumoniae, and other Enterobacteriaceae family members. In this study, 19 Klebsiella oxytoca and 5 Klebsiella pneumoniae isolates were isolated from cheese and minced meat samples using standard biochemical tests and identification kit. Biofilm formation in these isolates was detected by using microplate, Congo red agar, and tube adherence methods. Antibiotic susceptibility testing was performed using the disk diffusion method on Muller-Hinton agar. Using a microplate method strong biofilm formation was observed in 16 (84%) and 5 (100%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose respectively. After 24 and 48 hours of incubation on Tryptic Soy Broth without glucose strong biofilm formation was detected in 10 (52.6%) and 2 (40%) isolates of Klebsiella oxytoca and Klebsiella pneumoniae respectively. After 24 hours of incubation on Congo red agar 14 (73.7%) and 3 (60%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were slime factor positive respectively. In tube adherence method 13 (68.4%) and 4 (80%), isolates of Klebsiella oxytoca and Klebsiella pneumoniae were seen to have adhered strongly after 24 hours of incubation on Tryptic Soy Broth medium containing 2% of glucose. In general, strong biofilm formation by these strains was seen on Tryptic Soy Broth medium when supplemented with glucose. Among all the Klebsiella oxytoca, the highest rates of susceptibility were seen toward Trimethoprim-Sulfamethoxazole (100%) and Imipenem (94.7%) followed by Chloramphenicol (73.7%) and gentamicin (68.4%). Among 19 Klebsiella oxytoca isolates, the highest rates of resistance were seen in streptomycin (73.7%) and Kanamycin (73.7%) followed by ampicillin (63.2%). The majority of Klebsiella pneumoniae isolates were resistant to Kanamycin (80%) and Streptomycin (80%) followed by Amikacin (60%). On the other hand, 80% of Klebsiella pneumoniae were susceptible to imipenem, chloramphenicol, Trimethoprim-Sulfamethoxazole, and cefotaxime. Generally, majority of Klebsiella oxytoca, and Klebsiella pneumoniae isolates showed strong biofilm production on different growth conditions and majority of the isolates were also resistance for antibiotics. Therefore, biofilm production by these nosocomial bacteria has an implication public health and pave the way for increased resistance of biofilm-associated organisms to antimicrobial agents.
VL  - 5
IS  - 3
ER  -

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