@article{10.11648/j.jctr.20231103.11,
  author = {Yuan Mu and Xinyue Wang and Wenqiu Cai and Xiaojing Liu and Qian Zou and Tiantian Li and Wei Du and Hongyan Zhan and Sheng Tan and Hanmei Xu and Mengwei Li},
  title = {Regulating Oncogenic lncRNA VPS9D1-AS1 with Cholesterol-Modified siRNA for Esophageal Squamous Cell Carcinoma Therapy},
  journal = {Journal of Cancer Treatment and Research},
  volume = {11},
  number = {3},
  pages = {23-31},
  doi = {10.11648/j.jctr.20231103.11},
  url = {https://doi.org/10.11648/j.jctr.20231103.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jctr.20231103.11},
  abstract = {Esophageal carcinoma is the tenth most common cancer in the world. long non-coding RNA (lncRNA) is a type of RNA molecule that has no or limited coding ability. Many studies have found that it plays an important role in the occurrence and development of tumors. This article aims to investigate the expression and clinical significance of lncRNA VPS9D1-AS1 in patients with esophageal squamous cell carcinoma (ESCC). Additionally, it aims to examine the effects of targeted silencing of VPS9D1-AS1 on the growth and migration of ESCC cells in vivo and in vitro, and to explore the mechanism of action. This study compared the expression difference of lncRNA VPS9D1-AS1 in ESCC tissues and normal tissues through TCGA and UCSC databases, and analyzed the correlation between VPS9D1-AS1 expression and the prognosis of ESCC patients; clinical samples from ESCC patients were collected and qRT-PCR (Fluorescent quantitative polymerase chain reaction) was used to detect the relative expression of VPS9D1-AS1 and analyze the correlation between the expression of VPS9D1-AS1 and the onset and development of ESCC. Specific small interfering RNA was used to knock down the expression of VPS9D1-AS1 in ESCC cells, and the effect of VPS9D1-AS1 on the proliferation and migration of esophageal squamous cell carcinoma cells was detected by CCK-8 and Transwell assays. A subcutaneous transplantation tumor model of ESCC cells was established, and cholesterol-modified si-VPS9D1-AS1 was injected around the tumor to detect the impact of VPS9D1-AS1 on ESCC tumor growth in vivo. Western blot was used to detect the expression of apoptosis-related proteins in the tumor tissues of mice in each group. Finally, it was found that lncRNA VPS9D1-AS1 was highly expressed in ESCC tissues and significantly correlated with the prognosis of ESCC patients. Knocking down VPS9D1-AS1 regulates the proliferation, migration, and apoptosis of ESCC cells, and inhibits the growth of transplanted tumors in vivo, indicating it is a potential diagnostic and therapeutic target for esophageal squamous cell carcinoma.},
 year = {2023}
}

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TY  - JOUR
T1  - Regulating Oncogenic lncRNA VPS9D1-AS1 with Cholesterol-Modified siRNA for Esophageal Squamous Cell Carcinoma Therapy
AU  - Yuan Mu
AU  - Xinyue Wang
AU  - Wenqiu Cai
AU  - Xiaojing Liu
AU  - Qian Zou
AU  - Tiantian Li
AU  - Wei Du
AU  - Hongyan Zhan
AU  - Sheng Tan
AU  - Hanmei Xu
AU  - Mengwei Li
Y1  - 2023/10/14
PY  - 2023
N1  - https://doi.org/10.11648/j.jctr.20231103.11
DO  - 10.11648/j.jctr.20231103.11
T2  - Journal of Cancer Treatment and Research
JF  - Journal of Cancer Treatment and Research
JO  - Journal of Cancer Treatment and Research
SP  - 23
EP  - 31
PB  - Science Publishing Group
SN  - 2376-7790
UR  - https://doi.org/10.11648/j.jctr.20231103.11
AB  - Esophageal carcinoma is the tenth most common cancer in the world. long non-coding RNA (lncRNA) is a type of RNA molecule that has no or limited coding ability. Many studies have found that it plays an important role in the occurrence and development of tumors. This article aims to investigate the expression and clinical significance of lncRNA VPS9D1-AS1 in patients with esophageal squamous cell carcinoma (ESCC). Additionally, it aims to examine the effects of targeted silencing of VPS9D1-AS1 on the growth and migration of ESCC cells in vivo and in vitro, and to explore the mechanism of action. This study compared the expression difference of lncRNA VPS9D1-AS1 in ESCC tissues and normal tissues through TCGA and UCSC databases, and analyzed the correlation between VPS9D1-AS1 expression and the prognosis of ESCC patients; clinical samples from ESCC patients were collected and qRT-PCR (Fluorescent quantitative polymerase chain reaction) was used to detect the relative expression of VPS9D1-AS1 and analyze the correlation between the expression of VPS9D1-AS1 and the onset and development of ESCC. Specific small interfering RNA was used to knock down the expression of VPS9D1-AS1 in ESCC cells, and the effect of VPS9D1-AS1 on the proliferation and migration of esophageal squamous cell carcinoma cells was detected by CCK-8 and Transwell assays. A subcutaneous transplantation tumor model of ESCC cells was established, and cholesterol-modified si-VPS9D1-AS1 was injected around the tumor to detect the impact of VPS9D1-AS1 on ESCC tumor growth in vivo. Western blot was used to detect the expression of apoptosis-related proteins in the tumor tissues of mice in each group. Finally, it was found that lncRNA VPS9D1-AS1 was highly expressed in ESCC tissues and significantly correlated with the prognosis of ESCC patients. Knocking down VPS9D1-AS1 regulates the proliferation, migration, and apoptosis of ESCC cells, and inhibits the growth of transplanted tumors in vivo, indicating it is a potential diagnostic and therapeutic target for esophageal squamous cell carcinoma.
VL  - 11
IS  - 3
ER  - 

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