Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes

Ehab El-Kady; Hoda El-Qudaby; Marwa Omran

doi:doi:10.11648/j.sjac.20190702.11

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Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes

Ehab El-Kady, Hoda El-Qudaby, Marwa Omran

Published in Science Journal of Analytical Chemistry (Volume 7, Issue 2)

Received: 28 February 2019 Accepted: 3 April 2019 Published: 7 May 2019

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Abstract

New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.

Published in	Science Journal of Analytical Chemistry (Volume 7, Issue 2)
DOI	10.11648/j.sjac.20190702.11
Page(s)	32-41
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Mephenesin, Pharmaceuticals, Biological Fluids, Glassy Carbon Electrode, Pencil Graphite Electrode, Voltammetry

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APA Style

Ehab El-Kady, Hoda El-Qudaby, Marwa Omran. (2019). Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Science Journal of Analytical Chemistry, 7(2), 32-41. https://doi.org/10.11648/j.sjac.20190702.11

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ACS Style

Ehab El-Kady; Hoda El-Qudaby; Marwa Omran. Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Sci. J. Anal. Chem. 2019, 7(2), 32-41. doi: 10.11648/j.sjac.20190702.11

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AMA Style

Ehab El-Kady, Hoda El-Qudaby, Marwa Omran. Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Sci J Anal Chem. 2019;7(2):32-41. doi: 10.11648/j.sjac.20190702.11

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@article{10.11648/j.sjac.20190702.11,
  author = {Ehab El-Kady and Hoda El-Qudaby and Marwa Omran},
  title = {Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes},
  journal = {Science Journal of Analytical Chemistry},
  volume = {7},
  number = {2},
  pages = {32-41},
  doi = {10.11648/j.sjac.20190702.11},
  url = {https://doi.org/10.11648/j.sjac.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20190702.11},
  abstract = {New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.},
 year = {2019}
}

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TY  - JOUR
T1  - Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes
AU  - Ehab El-Kady
AU  - Hoda El-Qudaby
AU  - Marwa Omran
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.sjac.20190702.11
DO  - 10.11648/j.sjac.20190702.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 32
EP  - 41
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20190702.11
AB  - New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.
VL  - 7
IS  - 2
ER  -

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Author Information

Ehab El-Kady

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
Hoda El-Qudaby

National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Marwa Omran

National Organization for Drug Control and Research (NODCAR), Giza, Egypt

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APA Style

Ehab El-Kady, Hoda El-Qudaby, Marwa Omran. (2019). Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Science Journal of Analytical Chemistry, 7(2), 32-41. https://doi.org/10.11648/j.sjac.20190702.11

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ACS Style

Ehab El-Kady; Hoda El-Qudaby; Marwa Omran. Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Sci. J. Anal. Chem. 2019, 7(2), 32-41. doi: 10.11648/j.sjac.20190702.11

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AMA Style

Ehab El-Kady, Hoda El-Qudaby, Marwa Omran. Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes. Sci J Anal Chem. 2019;7(2):32-41. doi: 10.11648/j.sjac.20190702.11

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@article{10.11648/j.sjac.20190702.11,
  author = {Ehab El-Kady and Hoda El-Qudaby and Marwa Omran},
  title = {Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes},
  journal = {Science Journal of Analytical Chemistry},
  volume = {7},
  number = {2},
  pages = {32-41},
  doi = {10.11648/j.sjac.20190702.11},
  url = {https://doi.org/10.11648/j.sjac.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20190702.11},
  abstract = {New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.},
 year = {2019}
}

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TY  - JOUR
T1  - Comparative Voltammetric Behavior and Determination of Mephenesin in Pure Form, Pharmaceuticals and Biological Fluids at Pencil Graphite and Glassy Carbon Electrodes
AU  - Ehab El-Kady
AU  - Hoda El-Qudaby
AU  - Marwa Omran
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.sjac.20190702.11
DO  - 10.11648/j.sjac.20190702.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 32
EP  - 41
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20190702.11
AB  - New voltammetric methods are introduced for the determination of a skeletal muscle relaxant namely; mephenesin (Mep) in its pure form with the application in the pharmaceutical preparation and biological fluids. Three voltammetric methods namely; cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using pencil graphite electrode (PGE) and glassy carbon electrode (GCE) were used. Voltammetric behavior of Mep was investigated in Britton Robinson (BR) buffer as electrolyte in pH range 2-10. CV produced one irreversible anodic peak revealing oxidation reaction in pH 6 as maximum pH value at 1.33 and 1.36 V using PGE and GCE, respectively. At PGE, DPV and SWV were investigated and linearity ranges were 18.02-119.07 and 18.02-70.0 µg/mL with correlation coefficient 0.9997 and 0.9995, LOD values were found to be 0.113 and 0.059 µg/mL, respectively. At GCE, linearity ranges were 9.05-44.39 and 4.54-65.78 µg/mL with correlation coefficient 0.9996 and 0.9999, LOD values were 0.553 and 0.865 µg/mL at DPV and SWV, respectively. The proposed methods were applied with good recovery in pharmaceutical preparation, human mother milk and urine. They were validated and revealed accurate and precise results.
VL  - 7
IS  - 2
ER  -

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