Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validate an HPLC method for the dosage of AZT and the search for its degradation products. The validated method was carried out on an octadecylsylanized column (250 mm × 4.6 mm, 5 μm) using a mobile phase which consisted of a methanol/phosphate buffer mixture at pH 7.5 (80/20 v/v). The flow rate was set at 1.5 ml/min for a column temperature of 50°C and the UV detection wavelength at 210 nm. Forced degradation studies were carried out under the conditions: acidic, alkaline, oxidizing, UV light and thermal, in order to determine the degradation profiles. The validation parameters which are the linearity domain, the limits of detection and quantification; repeatability and accuracy were satisfactory. All samples showed consistent azithromycin contents. However, related substances and degradation products have been found in certain specialties. The validation of the HPLC method gave reliable results which allowed the determination of AZT and the search for degradation products and impurities.
| Published in | Science Journal of Analytical Chemistry (Volume 12, Issue 1) |
| DOI | 10.11648/j.sjac.20241101.11 |
| Page(s) | 1-6 |
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
COVID-19, Azithromycin, Validation, HPLC, Degradation Products
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APA Style
Yao, M. A., Kpaibe, A. S., Yao, T. J. S., Able, N., Bony, N., et al. (2024). Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Science Journal of Analytical Chemistry, 12(1), 1-6. https://doi.org/10.11648/j.sjac.20241101.11
ACS Style
Yao, M. A.; Kpaibe, A. S.; Yao, T. J. S.; Able, N.; Bony, N., et al. Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Sci. J. Anal. Chem. 2024, 12(1), 1-6. doi: 10.11648/j.sjac.20241101.11
AMA Style
Yao MA, Kpaibe AS, Yao TJS, Able N, Bony N, et al. Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19. Sci J Anal Chem. 2024;12(1):1-6. doi: 10.11648/j.sjac.20241101.11
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Download@article{10.11648/j.sjac.20241101.11,
author = {Marcelle Adjoa Yao and Andre Sawa Kpaibe and Thodhekes Jean Simon Yao and Nina Able and Nicaise Bony and Christophe Amin and Michèle Aké},
title = {Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19},
journal = {Science Journal of Analytical Chemistry},
volume = {12},
number = {1},
pages = {1-6},
doi = {10.11648/j.sjac.20241101.11},
url = {https://doi.org/10.11648/j.sjac.20241101.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20241101.11},
abstract = {Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validate an HPLC method for the dosage of AZT and the search for its degradation products. The validated method was carried out on an octadecylsylanized column (250 mm × 4.6 mm, 5 μm) using a mobile phase which consisted of a methanol/phosphate buffer mixture at pH 7.5 (80/20 v/v). The flow rate was set at 1.5 ml/min for a column temperature of 50°C and the UV detection wavelength at 210 nm. Forced degradation studies were carried out under the conditions: acidic, alkaline, oxidizing, UV light and thermal, in order to determine the degradation profiles. The validation parameters which are the linearity domain, the limits of detection and quantification; repeatability and accuracy were satisfactory. All samples showed consistent azithromycin contents. However, related substances and degradation products have been found in certain specialties. The validation of the HPLC method gave reliable results which allowed the determination of AZT and the search for degradation products and impurities.
},
year = {2024}
}
TY - JOUR T1 - Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19 AU - Marcelle Adjoa Yao AU - Andre Sawa Kpaibe AU - Thodhekes Jean Simon Yao AU - Nina Able AU - Nicaise Bony AU - Christophe Amin AU - Michèle Aké Y1 - 2024/01/08 PY - 2024 N1 - https://doi.org/10.11648/j.sjac.20241101.11 DO - 10.11648/j.sjac.20241101.11 T2 - Science Journal of Analytical Chemistry JF - Science Journal of Analytical Chemistry JO - Science Journal of Analytical Chemistry SP - 1 EP - 6 PB - Science Publishing Group SN - 2376-8053 UR - https://doi.org/10.11648/j.sjac.20241101.11 AB - Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validate an HPLC method for the dosage of AZT and the search for its degradation products. The validated method was carried out on an octadecylsylanized column (250 mm × 4.6 mm, 5 μm) using a mobile phase which consisted of a methanol/phosphate buffer mixture at pH 7.5 (80/20 v/v). The flow rate was set at 1.5 ml/min for a column temperature of 50°C and the UV detection wavelength at 210 nm. Forced degradation studies were carried out under the conditions: acidic, alkaline, oxidizing, UV light and thermal, in order to determine the degradation profiles. The validation parameters which are the linearity domain, the limits of detection and quantification; repeatability and accuracy were satisfactory. All samples showed consistent azithromycin contents. However, related substances and degradation products have been found in certain specialties. The validation of the HPLC method gave reliable results which allowed the determination of AZT and the search for degradation products and impurities. VL - 12 IS - 1 ER -
Department of Analytical Chemistry and Bromatology, Felix Houphouët Boigny University, Abidjan, Ivory Coast; Drug Control Laboratory, National Public Health Laboratory, Abidjan, Ivory Coast
Department of Analytical Chemistry and Bromatology, Felix Houphouët Boigny University, Abidjan, Ivory Coast
Department of Analytical Chemistry and Bromatology, Felix Houphouët Boigny University, Abidjan, Ivory Coast
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