Research Article
Quality Control by HPLC of Azithromycin Specialties Marketed in Ivory Coast During COVID-19
Marcelle Adjoa Yao*,
Andre Sawa Kpaibe,
Thodhekes Jean Simon Yao,
Nina Able,
Nicaise Bony,
Christophe Amin,
Michèle Aké
Issue:
Volume 12, Issue 1, April 2024
Pages:
1-6
Received:
20 November 2023
Accepted:
19 December 2023
Published:
8 January 2024
Abstract: Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validate an HPLC method for the dosage of AZT and the search for its degradation products. The validated method was carried out on an octadecylsylanized column (250 mm × 4.6 mm, 5 μm) using a mobile phase which consisted of a methanol/phosphate buffer mixture at pH 7.5 (80/20 v/v). The flow rate was set at 1.5 ml/min for a column temperature of 50°C and the UV detection wavelength at 210 nm. Forced degradation studies were carried out under the conditions: acidic, alkaline, oxidizing, UV light and thermal, in order to determine the degradation profiles. The validation parameters which are the linearity domain, the limits of detection and quantification; repeatability and accuracy were satisfactory. All samples showed consistent azithromycin contents. However, related substances and degradation products have been found in certain specialties. The validation of the HPLC method gave reliable results which allowed the determination of AZT and the search for degradation products and impurities.
Abstract: Azithromycin is an antibiotic of the macrolide subclass. Ivory Coast, in its strategy to combat the Covid-19 pandemic, has developed a treatment protocol including azithromycin. Faced with the high consumption of this molecule in the Covid-19 period and with the aim of fighting against substandard drugs, this work was undertaken in order to validat...
Show More
Review Article
Comparisons of Different Digestion Methods for Heavy Metal Analysis from Fruits
Issue:
Volume 12, Issue 1, March 2024
Pages:
7-12
Received:
18 January 2024
Accepted:
5 February 2024
Published:
13 March 2024
Abstract: Fruit juices are produced in home or industrially from horticultural crops by pressing the liquid part. They are rich in sugar, vitamins, and minerals like iron, copper, potassium, folate minerals, and vitamins A, B, and C which are essential for giving the body the nutrients it needs to stay healthy since fruits contain vital mineral components like copper (Cu), iron (Fe), and manganese (Mn), which is necessary for human growth and respiration. However, they may have heavy metals which may poison health risk and toxic even the presence is in little amount. Since fruit juices doesn’t pass through different processes, except extracting the liquid from the fruits of vegetables contamination and heavy metals affect human health. Before determination of heavy metals different procedures are applied for analysis. Digestion is the key component for determination of heavy metals from different samples. In this paper we are concerned on wet digestion methods for analysis. Closed system wet digestion is preferred since it lower the risk of contamination. There are different wet digestion types. Some of them are conventional wet digestion, ultraviolet digestion, ultrasound-assisted acid decomposition, conventional heating, microwave-assisted wet digestion etc. From thus, microwave digestion procedure was preferred for the digestion of samples for determination of heavy metals due to its ability to oxidize almost all of the organic samples.
Abstract: Fruit juices are produced in home or industrially from horticultural crops by pressing the liquid part. They are rich in sugar, vitamins, and minerals like iron, copper, potassium, folate minerals, and vitamins A, B, and C which are essential for giving the body the nutrients it needs to stay healthy since fruits contain vital mineral components li...
Show More
