Research Article
Gastroprotective and Antigastric Ulcer Effects of the Methanol Fraction of Cleistopholis Patens Leaves on Two Ulcer Models in Rats
Issue:
Volume 13, Issue 3, September 2025
Pages:
61-78
Received:
14 March 2025
Accepted:
26 March 2025
Published:
4 July 2025
Abstract: Background: Cleistopholis patens leaves are used to treat stomach disorders in Nigeria. This research investigated the gastroprotective and antigastric ulcer effects of the methanol fraction of Cleistopholis patens leaves (MFCPL) in rats. Methods: In vitro experiments involved DPPH-radical scavenging and H+/K+-ATPase inhibitory activities. Two ulcer models, diclofenac sodium (DS) (using 50 mg/kg b.w. DS orally) and acidified ethanol (AE) (using 0.3 M HCl + 60% ethanol orally), were used. The experimental rats (n=24) were grouped into 6 of 4 groups for each of the DS and AE ulcer models. In the DS model, group 1 served as the normal group, whereas group 2 was ulcer-induced without treatment. Group 3 received 20 mg/kg b.w. omeprazole, whereas groups 4, 5 and 6 received 50, 100 and 200 mg/kg b.w. MFCPL, respectively, for 14 days. For the AE ulcer model, 7 days after pretreatment with MFCPL, all groups except group 1 received acidified ethanol and were sacrificed after 1 hr. The ulcer indices, gastric mucosa morphology and serum antioxidant status of the rats were evaluated. Additionally, GC‒MS analyses of MFCPL were carried out. Results: The MFCPL had H+, K+-ATPase-inhibiting and DPPH-radical-scavenging activities, with half-maximal inhibitory concentrations (IC50) of 50.10 ± 10.52 μg/ml and 16.72 ± 2.47 μg/ml, respectively. Compared with the ulcer control, the administration of MFCPL led to a significant (p < 0.05) reduction in total ulcer counts and gastric volumes with high pH. Compared with those in the control group, the number of gastric lesions in the treated group was markedly lower than that in the control group. The antioxidant status of the treated rats improved. GC‒MS analyses of the MFCPL revealed 20 bioactive compounds. Conclusion: MCPL has an antigastric ulcer effect attributable to its H+/K+-ATPase inhibition and antioxidant activities due to its rich bioactive components and hence can be used to manage ulcers and their associated biochemical aberrations.
Abstract: Background: Cleistopholis patens leaves are used to treat stomach disorders in Nigeria. This research investigated the gastroprotective and antigastric ulcer effects of the methanol fraction of Cleistopholis patens leaves (MFCPL) in rats. Methods: In vitro experiments involved DPPH-radical scavenging and H+/K+-ATPase inhibitory activities. Two ulce...
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Research Article
Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats
Angbo Kousso Marie Angeline*,
Honoré St Seka Ohoueu
,
Adon Mousan Arsène,
Apoline Jeannette Aka Adouko,
Phillipe Alain Dit Bidié,
Okpèkon Aboua Timothée
Issue:
Volume 13, Issue 3, September 2025
Pages:
79-86
Received:
16 June 2025
Accepted:
27 June 2025
Published:
18 July 2025
Abstract: Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical screening and thin-layer chromatography. Acute and sub-acute toxicity tests were carried out in accordance with OECD protocols 423 and 407 respectively. Blood samples were taken on days 14 and 28 for analysis of biochemical parameters. Urea was determined using the enzymatic method described by Tietz. Creatinine was determined using the method of Jaffé. Total plasma protein concentration called the “biuret method” developed by Kronh. Alanine aminotransferase activity was determined according to the method recommended by the International Federation of Clinical Chemistry (IFCC). Aspartate aminotransferase (ASAT) activity was determined according to the method of ECCLS. Results: Phytochemistry showed the qualitative and expressive presence of polyphenols, flavonoids, tannins, phenolic acids and saponosides. For acute toxicity, somnolence was reported. No mortality or morbidity was observed. The LD50 was greater than 5000 mg/kg. Blood results showed no temporal disturbance in the functioning of biochemical markers of kidney, liver and heart, but some marginal variations were observed. Conclusion: The non-toxic effect revealed is proportional to the dose and active substances of the aqueous extract of Nauclea latifolia stem bark. This experiment would enable us to formulate improved traditional medicines to help treat oral cavity disorders.
Abstract: Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical...
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