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Research Article
Gastroprotective and Antigastric Ulcer Effects of the Methanol Fraction of Cleistopholis Patens Leaves on Two Ulcer Models in Rats
Issue:
Volume 13, Issue 3, September 2025
Pages:
61-78
Received:
14 March 2025
Accepted:
26 March 2025
Published:
4 July 2025
Abstract: Background: Cleistopholis patens leaves are used to treat stomach disorders in Nigeria. This research investigated the gastroprotective and antigastric ulcer effects of the methanol fraction of Cleistopholis patens leaves (MFCPL) in rats. Methods: In vitro experiments involved DPPH-radical scavenging and H+/K+-ATPase inhibitory activities. Two ulcer models, diclofenac sodium (DS) (using 50 mg/kg b.w. DS orally) and acidified ethanol (AE) (using 0.3 M HCl + 60% ethanol orally), were used. The experimental rats (n=24) were grouped into 6 of 4 groups for each of the DS and AE ulcer models. In the DS model, group 1 served as the normal group, whereas group 2 was ulcer-induced without treatment. Group 3 received 20 mg/kg b.w. omeprazole, whereas groups 4, 5 and 6 received 50, 100 and 200 mg/kg b.w. MFCPL, respectively, for 14 days. For the AE ulcer model, 7 days after pretreatment with MFCPL, all groups except group 1 received acidified ethanol and were sacrificed after 1 hr. The ulcer indices, gastric mucosa morphology and serum antioxidant status of the rats were evaluated. Additionally, GC‒MS analyses of MFCPL were carried out. Results: The MFCPL had H+, K+-ATPase-inhibiting and DPPH-radical-scavenging activities, with half-maximal inhibitory concentrations (IC50) of 50.10 ± 10.52 μg/ml and 16.72 ± 2.47 μg/ml, respectively. Compared with the ulcer control, the administration of MFCPL led to a significant (p < 0.05) reduction in total ulcer counts and gastric volumes with high pH. Compared with those in the control group, the number of gastric lesions in the treated group was markedly lower than that in the control group. The antioxidant status of the treated rats improved. GC‒MS analyses of the MFCPL revealed 20 bioactive compounds. Conclusion: MCPL has an antigastric ulcer effect attributable to its H+/K+-ATPase inhibition and antioxidant activities due to its rich bioactive components and hence can be used to manage ulcers and their associated biochemical aberrations.
Abstract: Background: Cleistopholis patens leaves are used to treat stomach disorders in Nigeria. This research investigated the gastroprotective and antigastric ulcer effects of the methanol fraction of Cleistopholis patens leaves (MFCPL) in rats. Methods: In vitro experiments involved DPPH-radical scavenging and H+/K+-ATPase inhibitory activities. Two ulce...
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Research Article
Photochemistry and Toxicity Studies of Aqueous Nauclea Latifolia Stem Bark Extract: Effect on Serum Biochemical Markers in Wistar Rats
Angbo Kousso Marie Angeline*,
Honoré St Seka Ohoueu
,
Adon Mousan Arsène,
Apoline Jeannette Aka Adouko,
Phillipe Alain Dit Bidié,
Okpèkon Aboua Timothée
Issue:
Volume 13, Issue 3, September 2025
Pages:
79-86
Received:
16 June 2025
Accepted:
27 June 2025
Published:
18 July 2025
Abstract: Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical screening and thin-layer chromatography. Acute and sub-acute toxicity tests were carried out in accordance with OECD protocols 423 and 407 respectively. Blood samples were taken on days 14 and 28 for analysis of biochemical parameters. Urea was determined using the enzymatic method described by Tietz. Creatinine was determined using the method of Jaffé. Total plasma protein concentration called the “biuret method” developed by Kronh. Alanine aminotransferase activity was determined according to the method recommended by the International Federation of Clinical Chemistry (IFCC). Aspartate aminotransferase (ASAT) activity was determined according to the method of ECCLS. Results: Phytochemistry showed the qualitative and expressive presence of polyphenols, flavonoids, tannins, phenolic acids and saponosides. For acute toxicity, somnolence was reported. No mortality or morbidity was observed. The LD50 was greater than 5000 mg/kg. Blood results showed no temporal disturbance in the functioning of biochemical markers of kidney, liver and heart, but some marginal variations were observed. Conclusion: The non-toxic effect revealed is proportional to the dose and active substances of the aqueous extract of Nauclea latifolia stem bark. This experiment would enable us to formulate improved traditional medicines to help treat oral cavity disorders.
Abstract: Bacground: Nauclea latifolia is a medicinal plant used in Côte d'Ivoire as a remedy for tooth decay, dental pain and mouth sores. Its consumption can be toxic. Objective: The aim of this study was to assess the renal, hepatic and myocardial effects, in order to prevent toxicity. Materials and methods: Phytochemistry was carried out by phytochemical...
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Research Article
Biochemical Monitoring in Oral and Maxillofacial Surgery: Feasibility and Application Perspectives in African Context
Nkolo Tolo Francis Daniel*,
Ama Moor Vicky Jocelyne
Issue:
Volume 13, Issue 3, September 2025
Pages:
87-92
Received:
3 July 2025
Accepted:
15 July 2025
Published:
30 July 2025
DOI:
10.11648/j.ab.20251303.13
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Abstract: Background: In sub-Saharan Africa, optimization of therapeutic protocols in maxillofacial surgery represents a major challenge faced with resource constraints, limited access to advanced imaging technologies, and complexity of pathologies. Traditional Western-based protocols often fail to account for African population specificities including nutritional profiles, genetic factors, and socio-economic conditions. Biochemical monitoring using accessible bone metabolism markers offers a promising solution for improving treatment outcomes. Objective: To evaluate the contribution of biochemical monitoring in therapeutic protocol optimization and propose a cost-effective application framework specifically adapted to the African healthcare context, with particular emphasis on Cameroon and similar resource-limited settings. Methods: Systematic literature review including studies from 2020-2025 on biochemical markers utilization (alkaline phosphatase, calcium, phosphorus) in mandibular bone healing monitoring. Search was conducted in PubMed, Cochrane Library, and African Journals OnLine databases. Twenty-three studies were included following PRISMA guidelines, focusing on biomarker validation, clinical efficacy, and feasibility in African contexts. Results: Studies demonstrate significant correlations between biochemical markers and bone mineral density (r=0.8-1.0, p<0.001). The Cameroon study shows perfect correlation (r=1.0) between alkaline phosphatase and bone mineral density. Monitoring integration allows 25% reduction in healing time (14.2±3.1 vs 18.7±4.8 weeks) and 40% decrease in post-operative complications (12.3% vs 20.8%). Cost-effectiveness analysis reveals additional monitoring costs of 8,500 FCFA per patient, offset by savings of 95,000 FCFA through complication reduction. Conclusions: Biochemical monitoring represents a promising, accessible approach to optimize maxillofacial surgery care in Africa. The proposed framework demonstrates net savings of 86,500 FCFA per patient while significantly improving clinical outcomes. Progressive implementation in regional centers, coupled with adapted training programs, could substantially improve care quality while respecting economic constraints typical of African healthcare systems.
Abstract: Background: In sub-Saharan Africa, optimization of therapeutic protocols in maxillofacial surgery represents a major challenge faced with resource constraints, limited access to advanced imaging technologies, and complexity of pathologies. Traditional Western-based protocols often fail to account for African population specificities including nutri...
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Research Article
Fructosamine Profile in Regular Blood Donors at the Bobo-Dioulasso Regional Blood Transfusion Centre, Burkina Faso
Issue:
Volume 13, Issue 3, September 2025
Pages:
93-99
Received:
4 July 2025
Accepted:
15 July 2025
Published:
30 July 2025
DOI:
10.11648/j.ab.20251303.14
Downloads:
Views:
Abstract: Introduction: Fructosamine is a stable ketoamine, formed by the reaction between glucose and the amino group of proteins (mainly albumin, but also globulins and lipoproteins). It indicates average blood glucose concentrations of 2 to 3 weeks for monitoring and controlling diabetes. However, studies on fructosamine based on the characteristics of Burkinabè are still rarely documented. With this in mind, the aim of this study was to investigate the fructosamine profile in regular blood donors from the town of Bobo-Dioulasso (Burkina Faso). Material and methods: This was a descriptive and analytical cross-sectional study with a collection period from 10 August 2024 to 10 September 2024. The study population consisted of fasting adult regular blood donors from Burkina Faso residing in the city of Bobo-Dioulasso, collected at the Bobo-Dioulasso Regional Blood Transfusion Centre. Fructosamine was determined by the colorimetric method using the Cobas® 6000 analyser. The data were analysed using R studio 4.3.3 software so that medians were calculated and reference values were determined at the 2.5 thand 97.5 thpercentile. Results-discussion: A total of 60 regular blood donors, equally distributed by gender, were selected. The mean age of the regular blood donors was 28.28±7.72 years (min = 19 years and max=52 years) while the mean number of donations was 6.97±8.50 (min=2 and max=61). Median fructosamine values were 272.5 µmol/L while the 2.5 thand 97.5 thpercentiles were 247.9 µmol/L and 314.77 µmol/L. The fructosamine ranges in our study population were higher than the reference values proposed by the reagent kit manufacturer and those proposed in the literature. There was no significant difference between the mean fructosamine concentrations according to sex, according to the age groups 19 to 36 and 37 to 55 and according to BMI (normal, overweight, obese). Conclusion: In a context of limited resources, this study contributes to providing reference ranges with a view to improving the control and monitoring of diabetes mellitus in Burkinabe subjects.
Abstract: Introduction: Fructosamine is a stable ketoamine, formed by the reaction between glucose and the amino group of proteins (mainly albumin, but also globulins and lipoproteins). It indicates average blood glucose concentrations of 2 to 3 weeks for monitoring and controlling diabetes. However, studies on fructosamine based on the characteristics of Bu...
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