Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico

Cynthia Mendoza-Rodriguez; Jorge Ocampo-Candiani; Pilar Morales-San Claudio; Osvaldo Vazquez-Martinez; Mauricio Salinas-Santander; Ernesto Torres-Lopez

doi:doi:10.11648/j.ijcems.20200605.12

Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico

Cynthia Mendoza-Rodriguez, Jorge Ocampo-Candiani, Pilar Morales-San Claudio, Osvaldo Vazquez-Martinez, Mauricio Salinas-Santander, Ernesto Torres-Lopez

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 6, Issue 5)

Received: 18 September 2020 Accepted: 5 October 2020 Published: 13 October 2020

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Abstract

Background Herpes simplex viruses 1 (human herpes virus types 1, HSV-1) often cause recurrent infections that affect the skin, mouth, lips, and eyes and eventually induce herpetic encephalitis. A high percentage of the population is infected with HSV-1 in which it produces a variety of these orofacial disease. In Mexico, there are no studies to determine the effects of viral virulence of clinical facial dermal isolates of active infections caused by the Herpes simplex virus 1. Objective of this work was to compare the herpetic activity of human clinical isolates from northeast Mexico against HSV-1 KOS as reference strain, which induces experimental murine model keratitis disease produced by infecting mouse corneas. Methods and Materials we compared several clinical isolate of HSV-1 obtained from 25 patients diagnosed with HSV-1 active, according to acyclovir (ACV) susceptibility, thymidine kinase (TK) polymerase chain reaction (PCR), and experimental Balb/c mice model as viral infections in vivo were evaluated. Results we found that several clinical isolates showed ACV resistance (48%) and pathogenic potential (PP) differences that caused ocular infection more or less than reference HSV-1 KOS strain. In Conclusion, some clinical isolate from northeast Mexico shown differences that caused ocular infection more or less than reference HSV-1 KOS strain.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 6, Issue 5)
DOI	10.11648/j.ijcems.20200605.12
Page(s)	91-95
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Herpes Simplex Virus, Virulence, Experimental Model, Clinical Isolates, Acyclovir, Northeast Mexico

References

[1]	Stannard LM, Fuller AO, Spear PG. Herpes simplex virus glycoproteins associated with different morphological entities projecting from the virion envelope. The Journal of general virology. 1987; 68 (Pt 3): 715-25. Epub 1987/03/01.
[2]	Mocarski Jr ES. Comparative analysis of herpesvirus-common proteins. In: Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, et al., editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge 2007.
[3]	David M. Knipe, Peter M. Howley, Diane E. Griffin, Robert A. Lamb, Malcolm A. Martin, Bernard Roizman, et al. Fundamental Virology. Harvard Medical School, Boston, MA: Lippincott Williams & Wilkins; 2001. p. 2399-459.
[4]	Al-Dujaili LJ, Clerkin PP, Clement C, McFerrin HE, Bhattacharjee PS, Varnell ED, et al. Ocular herpes simplex virus: how are latency, reactivation, recurrent disease and therapy interrelated? Future microbiology. 2011; 6 (8): 877-907. Epub 2011/08/25.
[5]	Irvine AR, Kimura SJ. Experimental stromal herpes simplex keratitis in rabbits. Transactions of the American Ophthalmological Society. 1967; 65: 189-210. Epub 1967/01/01.
[6]	Koskiniemi M, Piiparinen H, Mannonen L, Rantalaiho T, Vaheri A. Herpes encephalitis is a disease of middle aged and elderly people: polymerase chain reaction for detection of herpes simplex virus in the CSF of 516 patients with encephalitis. The Study Group. Journal of neurology, neurosurgery, and psychiatry. 1996; 60 (2): 174-8. Epub 1996/02/01.
[7]	Piacentini R, De Chiara G, Li Puma DD, Ripoli C, Marcocci ME, Garaci E, et al. HSV-1 and Alzheimer's disease: more than a hypothesis. Frontiers in pharmacology. 2014; 5: 97. Epub 2014/05/23.
[8]	Wang H, Davido DJ, Morrison LA. HSV-1 strain McKrae is more neuroinvasive than HSV-1 KOS after corneal or vaginal inoculation in mice. Virus research. 2013; 173 (2): 436-40. Epub 2013/01/24.
[9]	Koelle DM, Norberg P, Fitzgibbon MP, Russell RM, Greninger AL, Huang ML, et al. Worldwide circulation of HSV-2 x HSV-1 recombinant strains. Scientific reports. 2017; 7: 44084. Epub 2017/03/14.
[10]	Colgrove R, Diaz F, Newman R, Saif S, Shea T, Young S, et al. Genomic sequences of a low passage herpes simplex virus 2 clinical isolate and its plaque-purified derivative strain. Virology. 2014; 450-451: 140-5. Epub 2014/02/08.
[11]	Kolb AW, Schmidt TR, Dyer DW, Brandt CR. Sequence variation in the herpes simplex virus U (S) 1 ocular virulence determinant. Investigative ophthalmology & visual science. 2011; 52 (7): 4630-8. Epub 2011/04/27.
[12]	Morrison LA, Da Costa XJ, Knipe DM. Influence of mucosal and parenteral immunization with a replication-defective mutant of HSV-2 on immune responses and protection from genital challenge. Virology. 1998; 243 (1): 178-87. Epub 1998/04/07.
[13]	Morrison LA, Knipe DM. Immunization with replication-defective mutants of herpes simplex virus type 1: sites of immune intervention in pathogenesis of challenge virus infection. Journal of virology. 1994; 68 (2): 689-96. Epub 1994/02/01.
[14]	Korteweg C, Gu J. Pathology, molecular biology, and pathogenesis of avian influenza A (H5N1) infection in humans. The American journal of pathology. 2008; 172 (5): 1155-70. Epub 2008/04/12.
[15]	Bowen CD, Renner DW, Shreve JT, Tafuri Y, Payne KM, Dix RD, et al. Viral forensic genomics reveals the relatedness of classic herpes simplex virus strains KOS, KOS63, and KOS79. Virology. 2016; 492: 179-86. Epub 2016/03/08.
[16]	Szpara ML, Gatherer D, Ochoa A, Greenbaum B, Dolan A, Bowden RJ, et al. Evolution and diversity in human herpes simplex virus genomes. Journal of virology. 2014; 88 (2): 1209-27. Epub 2013/11/15.
[17]	Brunnemann AK, Liermann K, Deinhardt-Emmer S, Maschkowitz G, Pohlmann A, Sodeik B, et al. Recombinant herpes simplex virus type 1 strains with targeted mutations relevant for aciclovir susceptibility. Scientific reports. 2016; 6: 29903. Epub 2016/07/19.

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Cynthia Mendoza-Rodriguez, Jorge Ocampo-Candiani, Pilar Morales-San Claudio, Osvaldo Vazquez-Martinez, Mauricio Salinas-Santander, et al. (2020). Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. International Journal of Clinical and Experimental Medical Sciences, 6(5), 91-95. https://doi.org/10.11648/j.ijcems.20200605.12

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ACS Style

Cynthia Mendoza-Rodriguez; Jorge Ocampo-Candiani; Pilar Morales-San Claudio; Osvaldo Vazquez-Martinez; Mauricio Salinas-Santander, et al. Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. Int. J. Clin. Exp. Med. Sci. 2020, 6(5), 91-95. doi: 10.11648/j.ijcems.20200605.12

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AMA Style

Cynthia Mendoza-Rodriguez, Jorge Ocampo-Candiani, Pilar Morales-San Claudio, Osvaldo Vazquez-Martinez, Mauricio Salinas-Santander, et al. Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. Int J Clin Exp Med Sci. 2020;6(5):91-95. doi: 10.11648/j.ijcems.20200605.12

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@article{10.11648/j.ijcems.20200605.12,
  author = {Cynthia Mendoza-Rodriguez and Jorge Ocampo-Candiani and Pilar Morales-San Claudio and Osvaldo Vazquez-Martinez and Mauricio Salinas-Santander and Ernesto Torres-Lopez},
  title = {Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {6},
  number = {5},
  pages = {91-95},
  doi = {10.11648/j.ijcems.20200605.12},
  url = {https://doi.org/10.11648/j.ijcems.20200605.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20200605.12},
  abstract = {Background Herpes simplex viruses 1 (human herpes virus types 1, HSV-1) often cause recurrent infections that affect the skin, mouth, lips, and eyes and eventually induce herpetic encephalitis. A high percentage of the population is infected with HSV-1 in which it produces a variety of these orofacial disease. In Mexico, there are no studies to determine the effects of viral virulence of clinical facial dermal isolates of active infections caused by the Herpes simplex virus 1. Objective of this work was to compare the herpetic activity of human clinical isolates from northeast Mexico against HSV-1 KOS as reference strain, which induces experimental murine model keratitis disease produced by infecting mouse corneas. Methods and Materials we compared several clinical isolate of HSV-1 obtained from 25 patients diagnosed with HSV-1 active, according to acyclovir (ACV) susceptibility, thymidine kinase (TK) polymerase chain reaction (PCR), and experimental Balb/c mice model as viral infections in vivo were evaluated. Results we found that several clinical isolates showed ACV resistance (48%) and pathogenic potential (PP) differences that caused ocular infection more or less than reference HSV-1 KOS strain. In Conclusion, some clinical isolate from northeast Mexico shown differences that caused ocular infection more or less than reference HSV-1 KOS strain.},
 year = {2020}
}

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TY  - JOUR
T1  - Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico
AU  - Cynthia Mendoza-Rodriguez
AU  - Jorge Ocampo-Candiani
AU  - Pilar Morales-San Claudio
AU  - Osvaldo Vazquez-Martinez
AU  - Mauricio Salinas-Santander
AU  - Ernesto Torres-Lopez
Y1  - 2020/10/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ijcems.20200605.12
DO  - 10.11648/j.ijcems.20200605.12
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 91
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20200605.12
AB  - Background Herpes simplex viruses 1 (human herpes virus types 1, HSV-1) often cause recurrent infections that affect the skin, mouth, lips, and eyes and eventually induce herpetic encephalitis. A high percentage of the population is infected with HSV-1 in which it produces a variety of these orofacial disease. In Mexico, there are no studies to determine the effects of viral virulence of clinical facial dermal isolates of active infections caused by the Herpes simplex virus 1. Objective of this work was to compare the herpetic activity of human clinical isolates from northeast Mexico against HSV-1 KOS as reference strain, which induces experimental murine model keratitis disease produced by infecting mouse corneas. Methods and Materials we compared several clinical isolate of HSV-1 obtained from 25 patients diagnosed with HSV-1 active, according to acyclovir (ACV) susceptibility, thymidine kinase (TK) polymerase chain reaction (PCR), and experimental Balb/c mice model as viral infections in vivo were evaluated. Results we found that several clinical isolates showed ACV resistance (48%) and pathogenic potential (PP) differences that caused ocular infection more or less than reference HSV-1 KOS strain. In Conclusion, some clinical isolate from northeast Mexico shown differences that caused ocular infection more or less than reference HSV-1 KOS strain.
VL  - 6
IS  - 5
ER  -

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Author Information

Cynthia Mendoza-Rodriguez

Dermatology Service, Faculty of Medicine and Dr. Jose Eleuterio Gonzalez University Hospital, Autonomous University of Nuevo Leon, Nuevo León, Mexico
Jorge Ocampo-Candiani

Dermatology Service, Faculty of Medicine and Dr. Jose Eleuterio Gonzalez University Hospital, Autonomous University of Nuevo Leon, Nuevo León, Mexico
Pilar Morales-San Claudio

Microbiology Department, Center of Specialized Laboratories, Autonomous University of Nuevo Leon, Nuevo Leon, Mexico
Osvaldo Vazquez-Martinez

Dermatology Service, Faculty of Medicine and Dr. Jose Eleuterio Gonzalez University Hospital, Autonomous University of Nuevo Leon, Nuevo León, Mexico
Mauricio Salinas-Santander

Research Department, Faculty of Medicine, Autonomous University of Coahuila, Saltillo Coahuila, Mexico
Ernesto Torres-Lopez

Immunovirology Laboratory, Department of Immunology, Faculty of Medicine, Autonomous University of Nuevo Leon, Nuevo Leon, Mexico

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APA Style

Cynthia Mendoza-Rodriguez, Jorge Ocampo-Candiani, Pilar Morales-San Claudio, Osvaldo Vazquez-Martinez, Mauricio Salinas-Santander, et al. (2020). Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. International Journal of Clinical and Experimental Medical Sciences, 6(5), 91-95. https://doi.org/10.11648/j.ijcems.20200605.12

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ACS Style

Cynthia Mendoza-Rodriguez; Jorge Ocampo-Candiani; Pilar Morales-San Claudio; Osvaldo Vazquez-Martinez; Mauricio Salinas-Santander, et al. Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. Int. J. Clin. Exp. Med. Sci. 2020, 6(5), 91-95. doi: 10.11648/j.ijcems.20200605.12

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AMA Style

Cynthia Mendoza-Rodriguez, Jorge Ocampo-Candiani, Pilar Morales-San Claudio, Osvaldo Vazquez-Martinez, Mauricio Salinas-Santander, et al. Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico. Int J Clin Exp Med Sci. 2020;6(5):91-95. doi: 10.11648/j.ijcems.20200605.12

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@article{10.11648/j.ijcems.20200605.12,
  author = {Cynthia Mendoza-Rodriguez and Jorge Ocampo-Candiani and Pilar Morales-San Claudio and Osvaldo Vazquez-Martinez and Mauricio Salinas-Santander and Ernesto Torres-Lopez},
  title = {Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {6},
  number = {5},
  pages = {91-95},
  doi = {10.11648/j.ijcems.20200605.12},
  url = {https://doi.org/10.11648/j.ijcems.20200605.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20200605.12},
  abstract = {Background Herpes simplex viruses 1 (human herpes virus types 1, HSV-1) often cause recurrent infections that affect the skin, mouth, lips, and eyes and eventually induce herpetic encephalitis. A high percentage of the population is infected with HSV-1 in which it produces a variety of these orofacial disease. In Mexico, there are no studies to determine the effects of viral virulence of clinical facial dermal isolates of active infections caused by the Herpes simplex virus 1. Objective of this work was to compare the herpetic activity of human clinical isolates from northeast Mexico against HSV-1 KOS as reference strain, which induces experimental murine model keratitis disease produced by infecting mouse corneas. Methods and Materials we compared several clinical isolate of HSV-1 obtained from 25 patients diagnosed with HSV-1 active, according to acyclovir (ACV) susceptibility, thymidine kinase (TK) polymerase chain reaction (PCR), and experimental Balb/c mice model as viral infections in vivo were evaluated. Results we found that several clinical isolates showed ACV resistance (48%) and pathogenic potential (PP) differences that caused ocular infection more or less than reference HSV-1 KOS strain. In Conclusion, some clinical isolate from northeast Mexico shown differences that caused ocular infection more or less than reference HSV-1 KOS strain.},
 year = {2020}
}

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TY  - JOUR
T1  - Identification and Pathogenic Potential of Orofacial Herpetic Clinical Isolates in Northeast Mexico
AU  - Cynthia Mendoza-Rodriguez
AU  - Jorge Ocampo-Candiani
AU  - Pilar Morales-San Claudio
AU  - Osvaldo Vazquez-Martinez
AU  - Mauricio Salinas-Santander
AU  - Ernesto Torres-Lopez
Y1  - 2020/10/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ijcems.20200605.12
DO  - 10.11648/j.ijcems.20200605.12
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 91
EP  - 95
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20200605.12
AB  - Background Herpes simplex viruses 1 (human herpes virus types 1, HSV-1) often cause recurrent infections that affect the skin, mouth, lips, and eyes and eventually induce herpetic encephalitis. A high percentage of the population is infected with HSV-1 in which it produces a variety of these orofacial disease. In Mexico, there are no studies to determine the effects of viral virulence of clinical facial dermal isolates of active infections caused by the Herpes simplex virus 1. Objective of this work was to compare the herpetic activity of human clinical isolates from northeast Mexico against HSV-1 KOS as reference strain, which induces experimental murine model keratitis disease produced by infecting mouse corneas. Methods and Materials we compared several clinical isolate of HSV-1 obtained from 25 patients diagnosed with HSV-1 active, according to acyclovir (ACV) susceptibility, thymidine kinase (TK) polymerase chain reaction (PCR), and experimental Balb/c mice model as viral infections in vivo were evaluated. Results we found that several clinical isolates showed ACV resistance (48%) and pathogenic potential (PP) differences that caused ocular infection more or less than reference HSV-1 KOS strain. In Conclusion, some clinical isolate from northeast Mexico shown differences that caused ocular infection more or less than reference HSV-1 KOS strain.
VL  - 6
IS  - 5
ER  -

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