Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases

Li Zhencong; Xu Yude; Wu Hao; Yao Yicun; Ye Dongping; Miao Haixiong; Liang Weiguo

doi:doi:10.11648/j.ijcems.20210704.19

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Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases

Li Zhencong, Xu Yude, Wu Hao, Yao Yicun, Ye Dongping, Miao Haixiong, Liang Weiguo

Published in International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)

Received: 30 July 2021 Accepted: 10 August 2021 Published: 26 August 2021

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Abstract

Background: In the study of the pathogenesis of cartilage tissue (articular cartilage, meniscus, intervertebral disc, etc.) diseases, the most common research object is to explore some possible pathogenesis and pathogenesis in the direction of the whole pathological changes of these overall tissues. Therefore, there are many limitations and inaccuracies in the previous research methods. Single-cell sequencing refers to the technology of sequencing the genome and transcriptome epigenome at the single cell level, which can obtain the heterogeneity data between different cells from the sample tissue and sequence the precious very trace sample, so as to reflect the law and essence of life at a deeper level. Objective: Single cell sequencing technology can be used to explore the pathogenesis of cartilage tissue diseases, understand the relationship between a cell type and diseases and provide new ideas for treatment and diagnosis. Methods: The first author used the Chinese search terms "single-cell sequencing, cartilage, intervertebral disc, meniscus" and the English search terms "single-cell sequencing; cartilage; intervertebral disc; meniscus, osteoarthritis", relevant articles included between January 2010 and April 2021 were searched in Embase, PubMed, Wanfang database, and China National Knowledge Infrastructure (CNKI) database. The retrieved relevant literatures were screened and collated, and the included relevant literatures were reviewed in detail. Results & Conclusion: From the emergence of single-cell sequencing technology for the first time more than ten years ago, single-cell sequencing technology has been rapidly developed, and occupies an important position in the whole history of life science and technology development. Single-cell sequencing has been widely used in the study of tumor heterogeneity, immune microenvironment, neuroscience, embryonic development, and cell differentiation. However, the application in cartilage tissue diseases, is still in the primary stage of exploratory research. Single-cell sequencing technology can better understand the pathogenesis of cartilage tissue diseases at the cellular level, providing the possibility for pathological staging and targeted therapy at the molecular level of the disease. This is helpful for early diagnosis and early intervention of cartilage tissue diseases, reducing the pain caused by advanced cartilage tissue diseases and the economic burden of the whole society, so it also has a broad prospect in cartilage tissue diseases.

Published in	International Journal of Clinical and Experimental Medical Sciences (Volume 7, Issue 4)
DOI	10.11648/j.ijcems.20210704.19
Page(s)	120-126
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Single Cell Sequencing, Cartilage Tissue, Disc, Meniscus, Articular Cartilage, Osteoarthritis

References

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APA Style

Li Zhencong, Xu Yude, Wu Hao, Yao Yicun, Ye Dongping, et al. (2021). Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. International Journal of Clinical and Experimental Medical Sciences, 7(4), 120-126. https://doi.org/10.11648/j.ijcems.20210704.19

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ACS Style

Li Zhencong; Xu Yude; Wu Hao; Yao Yicun; Ye Dongping, et al. Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 120-126. doi: 10.11648/j.ijcems.20210704.19

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AMA Style

Li Zhencong, Xu Yude, Wu Hao, Yao Yicun, Ye Dongping, et al. Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. Int J Clin Exp Med Sci. 2021;7(4):120-126. doi: 10.11648/j.ijcems.20210704.19

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@article{10.11648/j.ijcems.20210704.19,
  author = {Li Zhencong and Xu Yude and Wu Hao and Yao Yicun and Ye Dongping and Miao Haixiong and Liang Weiguo},
  title = {Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {120-126},
  doi = {10.11648/j.ijcems.20210704.19},
  url = {https://doi.org/10.11648/j.ijcems.20210704.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.19},
  abstract = {Background: In the study of the pathogenesis of cartilage tissue (articular cartilage, meniscus, intervertebral disc, etc.) diseases, the most common research object is to explore some possible pathogenesis and pathogenesis in the direction of the whole pathological changes of these overall tissues. Therefore, there are many limitations and inaccuracies in the previous research methods. Single-cell sequencing refers to the technology of sequencing the genome and transcriptome epigenome at the single cell level, which can obtain the heterogeneity data between different cells from the sample tissue and sequence the precious very trace sample, so as to reflect the law and essence of life at a deeper level. Objective: Single cell sequencing technology can be used to explore the pathogenesis of cartilage tissue diseases, understand the relationship between a cell type and diseases and provide new ideas for treatment and diagnosis. Methods: The first author used the Chinese search terms "single-cell sequencing, cartilage, intervertebral disc, meniscus" and the English search terms "single-cell sequencing; cartilage; intervertebral disc; meniscus, osteoarthritis", relevant articles included between January 2010 and April 2021 were searched in Embase, PubMed, Wanfang database, and China National Knowledge Infrastructure (CNKI) database. The retrieved relevant literatures were screened and collated, and the included relevant literatures were reviewed in detail. Results & Conclusion: From the emergence of single-cell sequencing technology for the first time more than ten years ago, single-cell sequencing technology has been rapidly developed, and occupies an important position in the whole history of life science and technology development. Single-cell sequencing has been widely used in the study of tumor heterogeneity, immune microenvironment, neuroscience, embryonic development, and cell differentiation. However, the application in cartilage tissue diseases, is still in the primary stage of exploratory research. Single-cell sequencing technology can better understand the pathogenesis of cartilage tissue diseases at the cellular level, providing the possibility for pathological staging and targeted therapy at the molecular level of the disease. This is helpful for early diagnosis and early intervention of cartilage tissue diseases, reducing the pain caused by advanced cartilage tissue diseases and the economic burden of the whole society, so it also has a broad prospect in cartilage tissue diseases.},
 year = {2021}
}

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TY  - JOUR
T1  - Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases
AU  - Li Zhencong
AU  - Xu Yude
AU  - Wu Hao
AU  - Yao Yicun
AU  - Ye Dongping
AU  - Miao Haixiong
AU  - Liang Weiguo
Y1  - 2021/08/26
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210704.19
DO  - 10.11648/j.ijcems.20210704.19
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210704.19
AB  - Background: In the study of the pathogenesis of cartilage tissue (articular cartilage, meniscus, intervertebral disc, etc.) diseases, the most common research object is to explore some possible pathogenesis and pathogenesis in the direction of the whole pathological changes of these overall tissues. Therefore, there are many limitations and inaccuracies in the previous research methods. Single-cell sequencing refers to the technology of sequencing the genome and transcriptome epigenome at the single cell level, which can obtain the heterogeneity data between different cells from the sample tissue and sequence the precious very trace sample, so as to reflect the law and essence of life at a deeper level. Objective: Single cell sequencing technology can be used to explore the pathogenesis of cartilage tissue diseases, understand the relationship between a cell type and diseases and provide new ideas for treatment and diagnosis. Methods: The first author used the Chinese search terms "single-cell sequencing, cartilage, intervertebral disc, meniscus" and the English search terms "single-cell sequencing; cartilage; intervertebral disc; meniscus, osteoarthritis", relevant articles included between January 2010 and April 2021 were searched in Embase, PubMed, Wanfang database, and China National Knowledge Infrastructure (CNKI) database. The retrieved relevant literatures were screened and collated, and the included relevant literatures were reviewed in detail. Results & Conclusion: From the emergence of single-cell sequencing technology for the first time more than ten years ago, single-cell sequencing technology has been rapidly developed, and occupies an important position in the whole history of life science and technology development. Single-cell sequencing has been widely used in the study of tumor heterogeneity, immune microenvironment, neuroscience, embryonic development, and cell differentiation. However, the application in cartilage tissue diseases, is still in the primary stage of exploratory research. Single-cell sequencing technology can better understand the pathogenesis of cartilage tissue diseases at the cellular level, providing the possibility for pathological staging and targeted therapy at the molecular level of the disease. This is helpful for early diagnosis and early intervention of cartilage tissue diseases, reducing the pain caused by advanced cartilage tissue diseases and the economic burden of the whole society, so it also has a broad prospect in cartilage tissue diseases.
VL  - 7
IS  - 4
ER  -

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Author Information

Li Zhencong

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Xu Yude

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Wu Hao

School of Clinical Medicine, Guizhou Medical University, Guiyang, China
Yao Yicun

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Ye Dongping

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Miao Haixiong

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Liang Weiguo

Department of Orthopedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China

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APA Style

Li Zhencong, Xu Yude, Wu Hao, Yao Yicun, Ye Dongping, et al. (2021). Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. International Journal of Clinical and Experimental Medical Sciences, 7(4), 120-126. https://doi.org/10.11648/j.ijcems.20210704.19

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ACS Style

Li Zhencong; Xu Yude; Wu Hao; Yao Yicun; Ye Dongping, et al. Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. Int. J. Clin. Exp. Med. Sci. 2021, 7(4), 120-126. doi: 10.11648/j.ijcems.20210704.19

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AMA Style

Li Zhencong, Xu Yude, Wu Hao, Yao Yicun, Ye Dongping, et al. Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases. Int J Clin Exp Med Sci. 2021;7(4):120-126. doi: 10.11648/j.ijcems.20210704.19

Copy | Download

@article{10.11648/j.ijcems.20210704.19,
  author = {Li Zhencong and Xu Yude and Wu Hao and Yao Yicun and Ye Dongping and Miao Haixiong and Liang Weiguo},
  title = {Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases},
  journal = {International Journal of Clinical and Experimental Medical Sciences},
  volume = {7},
  number = {4},
  pages = {120-126},
  doi = {10.11648/j.ijcems.20210704.19},
  url = {https://doi.org/10.11648/j.ijcems.20210704.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20210704.19},
  abstract = {Background: In the study of the pathogenesis of cartilage tissue (articular cartilage, meniscus, intervertebral disc, etc.) diseases, the most common research object is to explore some possible pathogenesis and pathogenesis in the direction of the whole pathological changes of these overall tissues. Therefore, there are many limitations and inaccuracies in the previous research methods. Single-cell sequencing refers to the technology of sequencing the genome and transcriptome epigenome at the single cell level, which can obtain the heterogeneity data between different cells from the sample tissue and sequence the precious very trace sample, so as to reflect the law and essence of life at a deeper level. Objective: Single cell sequencing technology can be used to explore the pathogenesis of cartilage tissue diseases, understand the relationship between a cell type and diseases and provide new ideas for treatment and diagnosis. Methods: The first author used the Chinese search terms "single-cell sequencing, cartilage, intervertebral disc, meniscus" and the English search terms "single-cell sequencing; cartilage; intervertebral disc; meniscus, osteoarthritis", relevant articles included between January 2010 and April 2021 were searched in Embase, PubMed, Wanfang database, and China National Knowledge Infrastructure (CNKI) database. The retrieved relevant literatures were screened and collated, and the included relevant literatures were reviewed in detail. Results & Conclusion: From the emergence of single-cell sequencing technology for the first time more than ten years ago, single-cell sequencing technology has been rapidly developed, and occupies an important position in the whole history of life science and technology development. Single-cell sequencing has been widely used in the study of tumor heterogeneity, immune microenvironment, neuroscience, embryonic development, and cell differentiation. However, the application in cartilage tissue diseases, is still in the primary stage of exploratory research. Single-cell sequencing technology can better understand the pathogenesis of cartilage tissue diseases at the cellular level, providing the possibility for pathological staging and targeted therapy at the molecular level of the disease. This is helpful for early diagnosis and early intervention of cartilage tissue diseases, reducing the pain caused by advanced cartilage tissue diseases and the economic burden of the whole society, so it also has a broad prospect in cartilage tissue diseases.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Progress in the Application of Single-cell Sequencing Technology in Cartilage Tissue Diseases
AU  - Li Zhencong
AU  - Xu Yude
AU  - Wu Hao
AU  - Yao Yicun
AU  - Ye Dongping
AU  - Miao Haixiong
AU  - Liang Weiguo
Y1  - 2021/08/26
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcems.20210704.19
DO  - 10.11648/j.ijcems.20210704.19
T2  - International Journal of Clinical and Experimental Medical Sciences
JF  - International Journal of Clinical and Experimental Medical Sciences
JO  - International Journal of Clinical and Experimental Medical Sciences
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2469-8032
UR  - https://doi.org/10.11648/j.ijcems.20210704.19
AB  - Background: In the study of the pathogenesis of cartilage tissue (articular cartilage, meniscus, intervertebral disc, etc.) diseases, the most common research object is to explore some possible pathogenesis and pathogenesis in the direction of the whole pathological changes of these overall tissues. Therefore, there are many limitations and inaccuracies in the previous research methods. Single-cell sequencing refers to the technology of sequencing the genome and transcriptome epigenome at the single cell level, which can obtain the heterogeneity data between different cells from the sample tissue and sequence the precious very trace sample, so as to reflect the law and essence of life at a deeper level. Objective: Single cell sequencing technology can be used to explore the pathogenesis of cartilage tissue diseases, understand the relationship between a cell type and diseases and provide new ideas for treatment and diagnosis. Methods: The first author used the Chinese search terms "single-cell sequencing, cartilage, intervertebral disc, meniscus" and the English search terms "single-cell sequencing; cartilage; intervertebral disc; meniscus, osteoarthritis", relevant articles included between January 2010 and April 2021 were searched in Embase, PubMed, Wanfang database, and China National Knowledge Infrastructure (CNKI) database. The retrieved relevant literatures were screened and collated, and the included relevant literatures were reviewed in detail. Results & Conclusion: From the emergence of single-cell sequencing technology for the first time more than ten years ago, single-cell sequencing technology has been rapidly developed, and occupies an important position in the whole history of life science and technology development. Single-cell sequencing has been widely used in the study of tumor heterogeneity, immune microenvironment, neuroscience, embryonic development, and cell differentiation. However, the application in cartilage tissue diseases, is still in the primary stage of exploratory research. Single-cell sequencing technology can better understand the pathogenesis of cartilage tissue diseases at the cellular level, providing the possibility for pathological staging and targeted therapy at the molecular level of the disease. This is helpful for early diagnosis and early intervention of cartilage tissue diseases, reducing the pain caused by advanced cartilage tissue diseases and the economic burden of the whole society, so it also has a broad prospect in cartilage tissue diseases.
VL  - 7
IS  - 4
ER  -

Copy | Download