In the present study, the analgesic and anti-inflammatory effects of alcoholic extract of Elaeagnus angustifolia L.(AEEA) in a model of CIA II-induced osteoarthritis in mice were investigated in vivo and in vitro. Production of inflammatory cytokines such as iL-1β, TNF-α, iL-6 as well as COX-2, iNOS by chondrocytes and synovial macrophages stimulates chondrocytes and produces metalloproteinases, especially MMP-3, MMP-1 and causes destruction of articular cartilage. Mice were divided into three groups: healthy, patient before treatment, and patient after treatment and infected OA using CIA II. After easy death, tryptophan blue cells were cultured using pentobarbital sodium. To determine the injectable doses of the extract, LD50 and LC50 of mouse cells were measured first. It was extracted from the blood of mice and converted to cDNA using a kit. Samples were analyzed by Real-Time PCR to express the expression of iL-1β, TNF-α, iL-6, COX-2, iNOS genes. Chondrocytes were treated with LPS in vitro to produce PGE2, NO. The PGE2 production of chondrocytes was measured by ELISA, and the NO production of chondrocytes was measured by chlorometric method. PGE2 and NO production was performed in three groups: chondrocytes, chondrocytes with LPS, and cells treated with LPS and AEEA. The results of gene expression in PGE2 were reduced by 89% and NO by 70%. The expression level of COX-2 and iNOS cytokines was significantly decreased after consumption of AEEA extract, and this mice was reported in chondrocytes with 89% decrease for COX-2 gene and 79% decrease for iNOS gene. Expression levels of iL-1β, TNF-α and iL-6 genes decreased by 76% (iL-1β), 89% (TNF-α) and 91% (iL-6), respectively, after consuming AEEA extract. Significant reduction in the expression of pro-inflammatory cytokines by AEEA demonstrates its analgesic and anti-inflammatory effects in mice with osteoarthritis. AEEA was able to effectively and dose-dependently reduce the production of PGE2, NO in LPS-stimulated chondrocytes (in vitro). It also reduces the expression of pro-inflammatory genes such as iL-1β, TNF-α, iL-6, COX-2, iNOS in the blood and plasma of mice with osteoarthritis in vivo.
| Published in | International Journal of Clinical and Experimental Medical Sciences (Volume 8, Issue 6) |
| DOI | 10.11648/j.ijcems.20220806.11 |
| Page(s) | 78-86 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Osteoarthritis, CIA II, Elaeagnus Angustifolia L., Anti-Inflammatory, iL-1β, TNF-α, COX-2, iNOS
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APA Style
Amir Akbarnejad Eshkalak, Ladan Keyvan. (2022). Evaluation of Alcoholic Extract of Elaeagnus Angustifolia L. in Diminishing Proinflammatory Genes in a Model of CA-II-Induced OA Mice. International Journal of Clinical and Experimental Medical Sciences, 8(6), 78-86. https://doi.org/10.11648/j.ijcems.20220806.11
ACS Style
Amir Akbarnejad Eshkalak; Ladan Keyvan. Evaluation of Alcoholic Extract of Elaeagnus Angustifolia L. in Diminishing Proinflammatory Genes in a Model of CA-II-Induced OA Mice. Int. J. Clin. Exp. Med. Sci. 2022, 8(6), 78-86. doi: 10.11648/j.ijcems.20220806.11
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Download@article{10.11648/j.ijcems.20220806.11,
author = {Amir Akbarnejad Eshkalak and Ladan Keyvan},
title = {Evaluation of Alcoholic Extract of Elaeagnus Angustifolia L. in Diminishing Proinflammatory Genes in a Model of CA-II-Induced OA Mice},
journal = {International Journal of Clinical and Experimental Medical Sciences},
volume = {8},
number = {6},
pages = {78-86},
doi = {10.11648/j.ijcems.20220806.11},
url = {https://doi.org/10.11648/j.ijcems.20220806.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcems.20220806.11},
abstract = {In the present study, the analgesic and anti-inflammatory effects of alcoholic extract of Elaeagnus angustifolia L.(AEEA) in a model of CIA II-induced osteoarthritis in mice were investigated in vivo and in vitro. Production of inflammatory cytokines such as iL-1β, TNF-α, iL-6 as well as COX-2, iNOS by chondrocytes and synovial macrophages stimulates chondrocytes and produces metalloproteinases, especially MMP-3, MMP-1 and causes destruction of articular cartilage. Mice were divided into three groups: healthy, patient before treatment, and patient after treatment and infected OA using CIA II. After easy death, tryptophan blue cells were cultured using pentobarbital sodium. To determine the injectable doses of the extract, LD50 and LC50 of mouse cells were measured first. It was extracted from the blood of mice and converted to cDNA using a kit. Samples were analyzed by Real-Time PCR to express the expression of iL-1β, TNF-α, iL-6, COX-2, iNOS genes. Chondrocytes were treated with LPS in vitro to produce PGE2, NO. The PGE2 production of chondrocytes was measured by ELISA, and the NO production of chondrocytes was measured by chlorometric method. PGE2 and NO production was performed in three groups: chondrocytes, chondrocytes with LPS, and cells treated with LPS and AEEA. The results of gene expression in PGE2 were reduced by 89% and NO by 70%. The expression level of COX-2 and iNOS cytokines was significantly decreased after consumption of AEEA extract, and this mice was reported in chondrocytes with 89% decrease for COX-2 gene and 79% decrease for iNOS gene. Expression levels of iL-1β, TNF-α and iL-6 genes decreased by 76% (iL-1β), 89% (TNF-α) and 91% (iL-6), respectively, after consuming AEEA extract. Significant reduction in the expression of pro-inflammatory cytokines by AEEA demonstrates its analgesic and anti-inflammatory effects in mice with osteoarthritis. AEEA was able to effectively and dose-dependently reduce the production of PGE2, NO in LPS-stimulated chondrocytes (in vitro). It also reduces the expression of pro-inflammatory genes such as iL-1β, TNF-α, iL-6, COX-2, iNOS in the blood and plasma of mice with osteoarthritis in vivo.},
year = {2022}
}
TY - JOUR T1 - Evaluation of Alcoholic Extract of Elaeagnus Angustifolia L. in Diminishing Proinflammatory Genes in a Model of CA-II-Induced OA Mice AU - Amir Akbarnejad Eshkalak AU - Ladan Keyvan Y1 - 2022/12/27 PY - 2022 N1 - https://doi.org/10.11648/j.ijcems.20220806.11 DO - 10.11648/j.ijcems.20220806.11 T2 - International Journal of Clinical and Experimental Medical Sciences JF - International Journal of Clinical and Experimental Medical Sciences JO - International Journal of Clinical and Experimental Medical Sciences SP - 78 EP - 86 PB - Science Publishing Group SN - 2469-8032 UR - https://doi.org/10.11648/j.ijcems.20220806.11 AB - In the present study, the analgesic and anti-inflammatory effects of alcoholic extract of Elaeagnus angustifolia L.(AEEA) in a model of CIA II-induced osteoarthritis in mice were investigated in vivo and in vitro. Production of inflammatory cytokines such as iL-1β, TNF-α, iL-6 as well as COX-2, iNOS by chondrocytes and synovial macrophages stimulates chondrocytes and produces metalloproteinases, especially MMP-3, MMP-1 and causes destruction of articular cartilage. Mice were divided into three groups: healthy, patient before treatment, and patient after treatment and infected OA using CIA II. After easy death, tryptophan blue cells were cultured using pentobarbital sodium. To determine the injectable doses of the extract, LD50 and LC50 of mouse cells were measured first. It was extracted from the blood of mice and converted to cDNA using a kit. Samples were analyzed by Real-Time PCR to express the expression of iL-1β, TNF-α, iL-6, COX-2, iNOS genes. Chondrocytes were treated with LPS in vitro to produce PGE2, NO. The PGE2 production of chondrocytes was measured by ELISA, and the NO production of chondrocytes was measured by chlorometric method. PGE2 and NO production was performed in three groups: chondrocytes, chondrocytes with LPS, and cells treated with LPS and AEEA. The results of gene expression in PGE2 were reduced by 89% and NO by 70%. The expression level of COX-2 and iNOS cytokines was significantly decreased after consumption of AEEA extract, and this mice was reported in chondrocytes with 89% decrease for COX-2 gene and 79% decrease for iNOS gene. Expression levels of iL-1β, TNF-α and iL-6 genes decreased by 76% (iL-1β), 89% (TNF-α) and 91% (iL-6), respectively, after consuming AEEA extract. Significant reduction in the expression of pro-inflammatory cytokines by AEEA demonstrates its analgesic and anti-inflammatory effects in mice with osteoarthritis. AEEA was able to effectively and dose-dependently reduce the production of PGE2, NO in LPS-stimulated chondrocytes (in vitro). It also reduces the expression of pro-inflammatory genes such as iL-1β, TNF-α, iL-6, COX-2, iNOS in the blood and plasma of mice with osteoarthritis in vivo. VL - 8 IS - 6 ER -
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