Evaluation of Alcoholic Extract of Elaeagnus Angustifolia L. in Diminishing Proinflammatory Genes in a Model of CA-II-Induced OA Mice
Amir Akbarnejad Eshkalak,
Ladan Keyvan
Issue:
Volume 8, Issue 6, November 2022
Pages:
78-86
Received:
28 October 2022
Accepted:
8 December 2022
Published:
27 December 2022
DOI:
10.11648/j.ijcems.20220806.11
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Abstract: In the present study, the analgesic and anti-inflammatory effects of alcoholic extract of Elaeagnus angustifolia L.(AEEA) in a model of CIA II-induced osteoarthritis in mice were investigated in vivo and in vitro. Production of inflammatory cytokines such as iL-1β, TNF-α, iL-6 as well as COX-2, iNOS by chondrocytes and synovial macrophages stimulates chondrocytes and produces metalloproteinases, especially MMP-3, MMP-1 and causes destruction of articular cartilage. Mice were divided into three groups: healthy, patient before treatment, and patient after treatment and infected OA using CIA II. After easy death, tryptophan blue cells were cultured using pentobarbital sodium. To determine the injectable doses of the extract, LD50 and LC50 of mouse cells were measured first. It was extracted from the blood of mice and converted to cDNA using a kit. Samples were analyzed by Real-Time PCR to express the expression of iL-1β, TNF-α, iL-6, COX-2, iNOS genes. Chondrocytes were treated with LPS in vitro to produce PGE2, NO. The PGE2 production of chondrocytes was measured by ELISA, and the NO production of chondrocytes was measured by chlorometric method. PGE2 and NO production was performed in three groups: chondrocytes, chondrocytes with LPS, and cells treated with LPS and AEEA. The results of gene expression in PGE2 were reduced by 89% and NO by 70%. The expression level of COX-2 and iNOS cytokines was significantly decreased after consumption of AEEA extract, and this mice was reported in chondrocytes with 89% decrease for COX-2 gene and 79% decrease for iNOS gene. Expression levels of iL-1β, TNF-α and iL-6 genes decreased by 76% (iL-1β), 89% (TNF-α) and 91% (iL-6), respectively, after consuming AEEA extract. Significant reduction in the expression of pro-inflammatory cytokines by AEEA demonstrates its analgesic and anti-inflammatory effects in mice with osteoarthritis. AEEA was able to effectively and dose-dependently reduce the production of PGE2, NO in LPS-stimulated chondrocytes (in vitro). It also reduces the expression of pro-inflammatory genes such as iL-1β, TNF-α, iL-6, COX-2, iNOS in the blood and plasma of mice with osteoarthritis in vivo.
Abstract: In the present study, the analgesic and anti-inflammatory effects of alcoholic extract of Elaeagnus angustifolia L.(AEEA) in a model of CIA II-induced osteoarthritis in mice were investigated in vivo and in vitro. Production of inflammatory cytokines such as iL-1β, TNF-α, iL-6 as well as COX-2, iNOS by chondrocytes and synovial macrophages stimulat...
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Clinical Representation of the Different Rheumatological Manifestations of Diabetes
Halidou Idrissa Abdoul-Rahamane,
Kawtar Nassar,
Saadia Janani
Issue:
Volume 8, Issue 6, November 2022
Pages:
87-91
Received:
25 March 2022
Accepted:
19 April 2022
Published:
13 January 2023
Abstract: The various rheumatological clinical manifestations related to diabetes constitute a group of pathologies secondary to diabetes, regardless of the type of diabetes (type 1 or type 2), in most cases the manifestations are discovered at an advanced stage of diabetes, on average after 10 years of evolution. The different conditions of this group are frequent and diverse because they affect the majority of patients followed for diabetes. They are most often unrecognized or under-diagnosed in current practice, due to their clinical presentation which is characterized by a great polymorphism. These different manifestations can affect several structures of the locomotor system (bones, tendons, muscles, nerves or even joints and their synovial membranes) and constitute a real problem of differential diagnosis with the most commonly encountered rheumatological conditions. These clinical manifestations are either directly related to diabetes (chronic sustained hyperglycemia) or indirectly related to the various complications of diabetes; their prevalence is therefore correlated with poor glycemic control and/or the occurrence of other complications of diabetes. In this article, we describe the main rheumatological manifestations, their clinical and paraclinical diagnostic methods, their evolutionary profiles and their prevention as well as the different management in practice, and this will be a reminder in order to improve the practitioner's attitude towards the different clinical presentations of these affections.
Abstract: The various rheumatological clinical manifestations related to diabetes constitute a group of pathologies secondary to diabetes, regardless of the type of diabetes (type 1 or type 2), in most cases the manifestations are discovered at an advanced stage of diabetes, on average after 10 years of evolution. The different conditions of this group are f...
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