Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss

Mushtaq Ahmad Bhat; Showkat Ahmad Bhat; Sheikh Bilal Ahmad; Wasim Qureshi; Sabhiya Majid; Aarif Ali; Ishraq Hussain; Tehseen Hassan; Muneeb U. Rehman; Manzoor R. Mir

doi:doi:10.11648/j.ijde.20170202.11

Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss

Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, Aarif Ali, Ishraq Hussain, Tehseen Hassan, Muneeb U. Rehman, Manzoor R. Mir

Published in International Journal of Diabetes and Endocrinology (Volume 2, Issue 2)

Received: 26 December 2016 Accepted: 17 March 2017 Published: 11 April 2017

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Abstract

Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.

Published in	International Journal of Diabetes and Endocrinology (Volume 2, Issue 2)
DOI	10.11648/j.ijde.20170202.11
Page(s)	19-25
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Diabetes Mellitus-2, Biochemical Parameters, MTHFR Gene Polymorphism

References

[1]	Tabassum Rashid, S. A Bhat, MohdUrfan et al. The lipid peroxidation and antioxidant status of type 2 diabetic patients in Kashmir (India). Int J Diabetes Dev Ctries10.1007/s13410-015-0320-5.
[2]	Diabetes Fact sheet N 312. WHO. 2013.
[3]	International Diabetes Foundation: Diabetes Atlas". Retrieved 4 April 2014. Vos, T; Flaxman, AD et al (Dec 15, 2012). "Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010".
[4]	Vos, T; Flaxman, AD et al (Dec 15, 2012). "Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010.
[5]	Diabetes Fact sheet N 312". WHO. 2014.
[6]	Elizabeth H. Harris, clinical diabetes, 2005 American diabetes association; management of dyslipidemia in adults with diabetes.
[7]	Blessing O, Idonije, Oloruntoba Festus and Olarewaju M Oluba; Research journal of medical sciences 2011.
[8]	Pancreas Transplantation". American Diabetes Association. Retrieved 9 April 2014.
[9]	Haffner SM, Mykkanen L, Festa A Burke JP et al 2000, Insulin resistant prediabetic subjects have more atherogenic risk factors than insulin sensitive pre-diabetic subjects: JAMA 263: 2893-2898.
[10]	Garvey WT Kwon S Zheng D Shaughnessy S Wallace PHutto A Pugh K Jenkins AJ 2003; Effects of insulin resistance and type 2 diabetes on lipoprotiens subclass particle size and conceration determined by nuclear magnetic resonance. diabetes 52: 453-562.
[11]	Goyette P, Sumner JS, Milos R, Duncan AM, Rosenblatt DS, Matthews RG, Rozen R (June 1994). "Human methylenetetrahydrofolatereductase: isolation of cDNA, mapping and mutation identification". Nat. Genet. 7 (2): 195–200. doi: 10.1038/ng0694-195. PMID 7920641.
[12]	Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GJ, den Heijer M, Kluijtmans LA, van den Heuvel LP et al. (May 1995). "A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolatereductase". Nat. Genet. 10 (1): 111–3. doi: 10.1038/ng0595-111. PMID 7647779.
[13]	Joseph Sambrook and David W. Russell 2001. DNA extracted by phenol-chloroform-protenase-K method. Molecular Cloning, 3rd edition: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
[14]	Ronald, M, Krauss, M. 2004. Lipids and Lipoproteins in Patients with type 2 Diabetes. Diabetes Care, 27: 1496-1504.
[15]	Elizabeth H. Harris, clinical diabetes, 2005 American diabetes association; management of dyslipidemia in adults with diabetes.
[16]	Movva S, Alluri RV, Venkata S, Vedicherla B, Vattam KK, Ahuja YR, Hasan Q. 2011, Association of methylene tetrahydrofolatereductase C677T genotype with type 2 diabetes mellitus. Genet Test Mol Biomarkers. 2011 Apr; 15 (4): 257-61.
[17]	Wang JG, Staessen JA. Genetic polymorphisms in the renin– angiotensin system: relevance for susceptibility to cardiovascular disease. Eur J Pharmacol 2000; 410: 289–302.
[18]	Weisberg IS, Jacques PF, Selhub J, et al. The 1298AﬁC polymorphism in methylenetetrahydrofolatereductase (MTHFR): in vitro expression and association with homocysteine. Atherosclerosis 2001; 156: 409–15.
[19]	Maeda M, Yamamoto I, Fukuda M, Motomura T, Nishida M, 2008, MTHFR gene polymorphism is susceptible to diabetic retinopathy but not to diabetic nephropathy in Japanese type 2 diabetic patients. J Diabetes Complications. 2008 Mar-Apr; 22 (2): 119-25.
[20]	Sun J, Xu Y, Zhu Y, et al 2005. Methylenetetrahydrofolatereductase gene polymorphism, homocysteine and risk of macroangiopathy type 2 diabetes mellitus. J Endocrinology Invest; 29: 814–20.
[21]	Abo E, lAsrar MA, Hamed AA, Akar N, Egin Y, Saied MMM. Methylenetetrahydrofolatereductase gene polymorphism in type 1 diabetes mellitus: Relationship to microvascular complications. Egypt J Med Hum Genet 2012; 13 (2): 139–46.
[22]	Fletcher O, Kessling AM. MTHFR association with arteriosclerotic vascular disease? Hum Genet 1998; 103: 11–21.
[23]	Cugini P, Baldoni F, De Rosa R, et al. Higher blood pressure load (baric impact) in normotensives with endothelial dysfunction. A paraphysiological status of ‘‘pre-hypertension’’. ClinTer 2002; 153: 309–15.
[24]	Chang YH, Fu WM, Wu YH, Yeh CJ, Huang CN, Shiau MY. Prevalence of reductase C677T and A1298C polymorphisms in Taiwanese patients with Type 2 diabetic mellitus. ClinBiochem 2011; 44 (17–18): 1370–4. 104.
[25]	Friedman G, Goldschmidt N, Friedlander Y, Ben-Yehuda A, Selhub J, Babaey S, et al. A common mutation A1298C in human reductase gene: association with plasma total homocysteine and folate concentration. J Nutr 2001; 129: 1656–61.

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APA Style

Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. (2017). Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. International Journal of Diabetes and Endocrinology, 2(2), 19-25. https://doi.org/10.11648/j.ijde.20170202.11

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ACS Style

Mushtaq Ahmad Bhat; Showkat Ahmad Bhat; Sheikh Bilal Ahmad; Wasim Qureshi; Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int. J. Diabetes Endocrinol. 2017, 2(2), 19-25. doi: 10.11648/j.ijde.20170202.11

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AMA Style

Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int J Diabetes Endocrinol. 2017;2(2):19-25. doi: 10.11648/j.ijde.20170202.11

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@article{10.11648/j.ijde.20170202.11,
  author = {Mushtaq Ahmad Bhat and Showkat Ahmad Bhat and Sheikh Bilal Ahmad and Wasim Qureshi and Sabhiya Majid and Aarif Ali and Ishraq Hussain and Tehseen Hassan and Muneeb U. Rehman and Manzoor R. Mir},
  title = {Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss},
  journal = {International Journal of Diabetes and Endocrinology},
  volume = {2},
  number = {2},
  pages = {19-25},
  doi = {10.11648/j.ijde.20170202.11},
  url = {https://doi.org/10.11648/j.ijde.20170202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20170202.11},
  abstract = {Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.},
 year = {2017}
}

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TY  - JOUR
T1  - Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss
AU  - Mushtaq Ahmad Bhat
AU  - Showkat Ahmad Bhat
AU  - Sheikh Bilal Ahmad
AU  - Wasim Qureshi
AU  - Sabhiya Majid
AU  - Aarif Ali
AU  - Ishraq Hussain
AU  - Tehseen Hassan
AU  - Muneeb U. Rehman
AU  - Manzoor R. Mir
Y1  - 2017/04/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ijde.20170202.11
DO  - 10.11648/j.ijde.20170202.11
T2  - International Journal of Diabetes and Endocrinology
JF  - International Journal of Diabetes and Endocrinology
JO  - International Journal of Diabetes and Endocrinology
SP  - 19
EP  - 25
PB  - Science Publishing Group
SN  - 2640-1371
UR  - https://doi.org/10.11648/j.ijde.20170202.11
AB  - Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.
VL  - 2
IS  - 2
ER  -

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Author Information

Mushtaq Ahmad Bhat

Clinical-Biochemistry, University of Kashmir, Srinagar, India
Showkat Ahmad Bhat

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Sheikh Bilal Ahmad

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Wasim Qureshi

Registrar Academics/Physician, Govt. Medical College, Srinagar, India
Sabhiya Majid

Department of Biochemistry, Govt. Medical College, Srinagar, India
Aarif Ali

Clinical-Biochemistry, University of Kashmir, Srinagar, India
Ishraq Hussain

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Tehseen Hassan

Department of Biochemistry, Govt. Medical College, Srinagar, India
Muneeb U. Rehman

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India
Manzoor R. Mir

Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

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APA Style

Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. (2017). Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. International Journal of Diabetes and Endocrinology, 2(2), 19-25. https://doi.org/10.11648/j.ijde.20170202.11

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ACS Style

Mushtaq Ahmad Bhat; Showkat Ahmad Bhat; Sheikh Bilal Ahmad; Wasim Qureshi; Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int. J. Diabetes Endocrinol. 2017, 2(2), 19-25. doi: 10.11648/j.ijde.20170202.11

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AMA Style

Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int J Diabetes Endocrinol. 2017;2(2):19-25. doi: 10.11648/j.ijde.20170202.11

Copy | Download

@article{10.11648/j.ijde.20170202.11,
  author = {Mushtaq Ahmad Bhat and Showkat Ahmad Bhat and Sheikh Bilal Ahmad and Wasim Qureshi and Sabhiya Majid and Aarif Ali and Ishraq Hussain and Tehseen Hassan and Muneeb U. Rehman and Manzoor R. Mir},
  title = {Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss},
  journal = {International Journal of Diabetes and Endocrinology},
  volume = {2},
  number = {2},
  pages = {19-25},
  doi = {10.11648/j.ijde.20170202.11},
  url = {https://doi.org/10.11648/j.ijde.20170202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijde.20170202.11},
  abstract = {Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.},
 year = {2017}
}

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TY  - JOUR
T1  - Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss
AU  - Mushtaq Ahmad Bhat
AU  - Showkat Ahmad Bhat
AU  - Sheikh Bilal Ahmad
AU  - Wasim Qureshi
AU  - Sabhiya Majid
AU  - Aarif Ali
AU  - Ishraq Hussain
AU  - Tehseen Hassan
AU  - Muneeb U. Rehman
AU  - Manzoor R. Mir
Y1  - 2017/04/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ijde.20170202.11
DO  - 10.11648/j.ijde.20170202.11
T2  - International Journal of Diabetes and Endocrinology
JF  - International Journal of Diabetes and Endocrinology
JO  - International Journal of Diabetes and Endocrinology
SP  - 19
EP  - 25
PB  - Science Publishing Group
SN  - 2640-1371
UR  - https://doi.org/10.11648/j.ijde.20170202.11
AB  - Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.
VL  - 2
IS  - 2
ER  -

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