Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of <i>Helicobacter Pylori</i> Isolates from South of Russia

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova

doi:doi:10.11648/j.ijmb.20170203.15

Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)

Received: 3 October 2016 Accepted: 19 March 2017 Published: 18 April 2017

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Abstract

High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.

Published in	International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)
DOI	10.11648/j.ijmb.20170203.15
Page(s)	135-138
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Helicobacter Pylori, VNTR Genotyping, MLVA, Cluster Analysis, Molecular Epidemiology

References

[1]	Achtman, M., T. Azuma, D. E. Berg, Y. Ito, G. Morelli, Z. J. Pan, S. Suerbaum, S. Thompson, A. van der Ende, and L. J. van Doorn. Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol. Microbiol, 1999; 32: 459–470.
[2]	Takami, S., T. Hayashi, H. Akashi, T. Shimoyama, and T. Tamura. Genetic heterogeneity of Helicobacter pylori by pulse-ﬁeld gel electrophoresis and re-evaluation of DNA homology. Eur. J. Gastroenterol. Hepatol. 1994. Suppl. 1: S53-S60.
[3]	Salaun, L., C. Audibert, G. Le Ley, C. Burucoa, J. L. Fauchere, and B. Picard. Panmictic structure of Helicobacter pylori demonstrated by the comparative study of six genetic markers. FEMS Microbiol. Lett. 1998, 161: 231–239.
[4]	Owen, R. J., J. Bickley, M. Costas, and D. R. Morgan. Genomic variation in Helicobacter pylori: application for identiﬁcation of strains. J. Gastroenterol, 1991, 181: 43–50.
[5]	Akopyantz, N., N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD ﬁngerprinting. Nucleic Acids Res, 1992, 20:5137–5142.
[6]	Gibson, J. R., E. Slater, J. Xerry, D. S. Tompkins, and R. J. Owen. Use of an ampliﬁed-fragment length polymorphism technique to ﬁngerprint and differentiate isolates of Helicobacter pylori. J. Clin. Microbiol, 1998. 36:2580–2585.
[7]	Ahmed, N., A. A. Khan, A. Alvi, S. Tiwari, C. S. Jyothirmayee, F. Kauser, M. Ali, and C. M. Habibullah. Genomic analysis of Helicobacter pylori from Andhra Pradesh, South India: molecular evidence for three major genetic clusters. Curr. Sci. 2003, 85: 101–108.
[8]	Miehlke, S., R. Thomas, O. Gutierrez, D. Y. Graham, and M. F. Go. DNA ﬁngerprinting of single colonies of Helicobacter pylori from gastric cancer patients suggests infection with a single predominant strain. J. Clin. Microbiol. 1999, 37: 245–247.
[9]	Nathalie, E., M. van Doorn, F. Namavar, L. J. van Doorn, Z. Durrani, E. J. Kuipers, and C. M. J. E. Vandenbroucke-Grauls. Analysis of vacA, cagA, and IS605 genotypes and those determined by PCR ampliﬁcation of DNA between repetitive sequences of Helicobacter pylori strains isolated from patients with nonulcer dyspepsia or mucosa-associated lymphoid tissue lymphoma. J. Clin. Microbiol. 1999, 37: 2348–2349.
[10]	Ann-Catrin, E. T., N. Hosseini, A. M. Svennerholm, and I. Bo ¨lin. Different Helicobacter pylori strains colonize the antral and duodenal mucosa of duodenal ulcer patients. Helicobacter, 2000, 5:69–78.
[11]	Falush D, Wirth T, Linz B, Pritchard JK, Stephens M, Kidd M, Blaser MJ, Graham DY, Vacher S, Perez-Perez GI, Yamaoka Y, Megraud F, Otto K, Reichard U, Katzowitsch E, Wang X, Achtman M, Suerbaum S, Traces of human migrations in Helicobacter pylori populations. Science, 2003, 299:1582-1585.
[12]	van Belkum A, Scherer S, van Alphen L, Verbrugh H. Short-sequence DNA repeats in prokaryotic genomes. Microbiol Mol Biol Rev, 1998, 62: 275-293.
[13]	Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res, 1999, 27: 573-580.
[14]	de Knegt, L. V., Pires, S. M., Löfström, C., Sørensen, G., Pedersen, K., Torpdahl, M., Nielsen, E. M. and Hald, T. Application of Molecular Typing Results in Source Attribution Models: The Case of Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) of Salmonella Isolates Obtained from Integrated Surveillance in Denmark. Risk Analysis, 2016, 36: 571–588. doi:10. 1111/risa. 12483.
[15]	Costa, N. S., Pinto, T. C., Merquior, V. L., Castro, L. F., da Rocha, F. S., Morais, J. M.,... & Teixeira, L. M.. MLVA Typing of Streptococcus pneumoniae Isolates with Emphasis on Serotypes 14, 9N and 9V: Comparison of Previously Described Panels and Proposal of a Novel 7 VNTR Loci-Based Simplified Scheme. PloS one, 2016, 11 (7), e0158651.
[16]	Guo, C., Liao, Y., Li, Y., Duan, J., Guo, Y., Wu,; & Guo, G. Genotyping analysis of Helicobacter pylori using multiple-locus variable-number tandem-repeats analysis in five regions of China and Japan. BMC microbiology, 2011, 11(1), 197.
[17]	Sorokin V. M., Pisanov R.V., Bereznyak E. A., Prozorova L. A. Development of multiple-locus variable number of tandem repeat analysis (MLVA) for Helicobacter pylori and its application to Helicobacter pylori isolates from Rostov region, Russia. Rev Electron Biomed / Electron J Biomed .2012; 3: 32-39.
[18]	Li W. H., Sadler L. A. Low nucleotide diversity in man. Genetics. 1991, 129: 513–523.
[19]	Momynaliev K. T., Chelysheva V. V., Akopian T. A. Population-based identification Russian isolates of H pylori. Genetika, 2005, 41(10):1-4. In Russian.
[20]	Kawai et al. Evolution in an oncogenic bacterial species with extreme genome plasticity: Helicobacter pylori East Asian genomes. BMC Microbiology 2011 11:104. doi:10. 1186/1471-2180-11-104.

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APA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. (2017). Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. International Journal of Microbiology and Biotechnology, 2(3), 135-138. https://doi.org/10.11648/j.ijmb.20170203.15

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ACS Style

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int. J. Microbiol. Biotechnol. 2017, 2(3), 135-138. doi: 10.11648/j.ijmb.20170203.15

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AMA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int J Microbiol Biotechnol. 2017;2(3):135-138. doi: 10.11648/j.ijmb.20170203.15

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@article{10.11648/j.ijmb.20170203.15,
  author = {Vladimir Mihajlovich Sorokin and Ruslan Vjacheslavovich Pisanov and Elena Vadimovna Golubkina and Elena Aleksandrovna Bereznyak and Lubov Aleksandrovna Prozorova},
  title = {Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {3},
  pages = {135-138},
  doi = {10.11648/j.ijmb.20170203.15},
  url = {https://doi.org/10.11648/j.ijmb.20170203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.15},
  abstract = {High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.},
 year = {2017}
}

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TY - JOUR
T1 - Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia
AU - Vladimir Mihajlovich Sorokin
AU - Ruslan Vjacheslavovich Pisanov
AU - Elena Vadimovna Golubkina
AU - Elena Aleksandrovna Bereznyak
AU - Lubov Aleksandrovna Prozorova
Y1 - 2017/04/18
PY - 2017
N1 - https://doi.org/10.11648/j.ijmb.20170203.15
DO - 10.11648/j.ijmb.20170203.15
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 135
EP - 138
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20170203.15
AB - High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.
VL - 2
IS - 3
ER -

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Author Information

Vladimir Mihajlovich Sorokin

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Ruslan Vjacheslavovich Pisanov

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Elena Vadimovna Golubkina

Department of Biochemistry, Astrakhan State Medical University, Ministry of Health of Russia, Astrahan', Russia
Elena Aleksandrovna Bereznyak

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Lubov Aleksandrovna Prozorova

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia

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APA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. (2017). Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. International Journal of Microbiology and Biotechnology, 2(3), 135-138. https://doi.org/10.11648/j.ijmb.20170203.15

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ACS Style

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int. J. Microbiol. Biotechnol. 2017, 2(3), 135-138. doi: 10.11648/j.ijmb.20170203.15

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AMA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int J Microbiol Biotechnol. 2017;2(3):135-138. doi: 10.11648/j.ijmb.20170203.15

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@article{10.11648/j.ijmb.20170203.15,
  author = {Vladimir Mihajlovich Sorokin and Ruslan Vjacheslavovich Pisanov and Elena Vadimovna Golubkina and Elena Aleksandrovna Bereznyak and Lubov Aleksandrovna Prozorova},
  title = {Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {3},
  pages = {135-138},
  doi = {10.11648/j.ijmb.20170203.15},
  url = {https://doi.org/10.11648/j.ijmb.20170203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.15},
  abstract = {High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.},
 year = {2017}
}

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