Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of <i>Merremia tridentata</i> Leaves

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall

doi:doi:10.11648/j.sjc.20221004.14

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Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall

Published in Science Journal of Chemistry (Volume 10, Issue 4)

Received: 3 August 2022 Accepted: 18 August 2022 Published: 29 August 2022

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Abstract

The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.

Published in	Science Journal of Chemistry (Volume 10, Issue 4)
DOI	10.11648/j.sjc.20221004.14
Page(s)	120-125
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Merremia tridentata, Extraction, Antioxidant, Polyphenols, Flavonoids, Proanthocyanidins

References

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APA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. (2022). Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Science Journal of Chemistry, 10(4), 120-125. https://doi.org/10.11648/j.sjc.20221004.14

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ACS Style

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci. J. Chem. 2022, 10(4), 120-125. doi: 10.11648/j.sjc.20221004.14

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AMA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci J Chem. 2022;10(4):120-125. doi: 10.11648/j.sjc.20221004.14

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@article{10.11648/j.sjc.20221004.14,
  author = {Papa Biram Sy and Aissatou Alioune Gaye and Alioune Fall},
  title = {Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves},
  journal = {Science Journal of Chemistry},
  volume = {10},
  number = {4},
  pages = {120-125},
  doi = {10.11648/j.sjc.20221004.14},
  url = {https://doi.org/10.11648/j.sjc.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20221004.14},
  abstract = {The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.},
 year = {2022}
}

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TY  - JOUR
T1  - Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves
AU  - Papa Biram Sy
AU  - Aissatou Alioune Gaye
AU  - Alioune Fall
Y1  - 2022/08/29
PY  - 2022
N1  - https://doi.org/10.11648/j.sjc.20221004.14
DO  - 10.11648/j.sjc.20221004.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 120
EP  - 125
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20221004.14
AB  - The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.
VL  - 10
IS  - 4
ER  -

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Author Information

Papa Biram Sy

Department of Chemistry, University Cheikh Anta Diop, Dakar, Senegal
Aissatou Alioune Gaye

Center for Food Security and Functional Molecules Studies, University Cheikh Anta Diop, Dakar, Senegal
Alioune Fall

Department of Chemistry, University Cheikh Anta Diop, Dakar, Senegal

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Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. (2022). Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Science Journal of Chemistry, 10(4), 120-125. https://doi.org/10.11648/j.sjc.20221004.14

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ACS Style

Papa Biram Sy; Aissatou Alioune Gaye; Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci. J. Chem. 2022, 10(4), 120-125. doi: 10.11648/j.sjc.20221004.14

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AMA Style

Papa Biram Sy, Aissatou Alioune Gaye, Alioune Fall. Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves. Sci J Chem. 2022;10(4):120-125. doi: 10.11648/j.sjc.20221004.14

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@article{10.11648/j.sjc.20221004.14,
  author = {Papa Biram Sy and Aissatou Alioune Gaye and Alioune Fall},
  title = {Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves},
  journal = {Science Journal of Chemistry},
  volume = {10},
  number = {4},
  pages = {120-125},
  doi = {10.11648/j.sjc.20221004.14},
  url = {https://doi.org/10.11648/j.sjc.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20221004.14},
  abstract = {The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.},
 year = {2022}
}

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TY  - JOUR
T1  - Assay of Total Polyphenols, Flavonoids, and Proanthocyanidins and Determination of the Antioxidant Activity of Different Extracts of Merremia tridentata Leaves
AU  - Papa Biram Sy
AU  - Aissatou Alioune Gaye
AU  - Alioune Fall
Y1  - 2022/08/29
PY  - 2022
N1  - https://doi.org/10.11648/j.sjc.20221004.14
DO  - 10.11648/j.sjc.20221004.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 120
EP  - 125
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20221004.14
AB  - The aqueous and organic solvent extracts of Merremia tridentata leaves are studied. Extraction conditions are optimized: an extraction time of 20 minutes, a temperature of 80°C and a plant material/water mixture of 1% give the best yield of polyphenol. The amount of polyphenols, flavonoids, and proanthocyanins of each organic solvent extract was investigated. The results obtained show that the aqueous extract is richer in phenolic compounds and in flavonoids with, respectively, a Total Polyphenolic Content (TPC) of (29.823±0.56 mg EQAG/g) and a Total Flavonoid Content (TFC) of 10.18±0.32 mg EQ/g. For the proanthocyanidins, it can be noted that all the extracts have substantially equal values varying between 0.6377-0.6906 mg/g). Antioxidant activities are measured using two different methods (DPPH· and CUPRAC). The aqueous extract have the highest values of (77.67±0.98 mg/g) and (96.37±2.02 mg/g), respectively. The study of the antioxidant activities of the extracts do not show a correlation between the amount of polyphenols and the IC50. The ethanolic and methanolic extracts which are although less rich in polyphenol, seem more effective with IC50 estimated at 0.108 mg/mL and 0.110 mg/mL respectively, while the aqueous extract, which is richer in polyphenol records an IC50 of 0.283 mg/mL.
VL  - 10
IS  - 4
ER  -

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