Hong-Chol Ri,Dong-Min Han,Kwang-Il To*,Yong Il Yun,Song-Nam Go,Chang-Guk Kim,Su-Chol Rim
Issue:
Volume 10, Issue 3, September 2025
Pages:
37-44
Received:
11 May 2025
Accepted:
11 June 2025
Published:
10 July 2025
DOI:
10.11648/j.bmb.20251003.11
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Abstract: Norcantharidin (NCTD) is a demethylated water-soluble synthetic small molecule of cantharidin (CTD), has been reported that it has anticancer activities including apoptosis and anti-angiogenesis. NCTD reduced VEGF production and the expression of integrin-β1 in tumor cells even at the low concentrations with less toxicity to the kidney or liver, and destroyed vimentin to inhibit angiogenesis in tumor cells by inducing anoikis of vascular endothelial cells. In addition, basing on recent data that the metastasis and recurrence of tumor are closely related to angiogenesis in tumor tissues, Croquel-180 sarcomatous cells, a mesenchyma-derived cells group with better angiogenesis were transplanted to Chick embryo Chorioallantoic Membrane (CAM) and the anti-angiogenic effect of NCTD was examined. When NCTD was injected at a dose of 40µg/µL and 80µg/µL, there was significant decrease in the number of microvascular branches than the control group. There was no significant difference in microvascular density (MVD) when NCTD was injected at a dose of 20µg/μL, but at a dose of 40µg/µL and 80µg/µL, MVD decreased significantly compared to the control. We reaffirmed that NCTD is a drug that can specifically inhibit angiogenesis in tumor tissues through CAM experiments, and that it is a very effective drug that can be applied to antitumor therapy targeting angiogenesis in tumor tissues. Norcantharidin is a powerful angiogenesis inhibitor capable of sufficiently inhibiting angiogenesis in tumor tissue. We confirmed that it has potential application value applicable to tumor treatment targeting endothelial cells.Abstract: Norcantharidin (NCTD) is a demethylated water-soluble synthetic small molecule of cantharidin (CTD), has been reported that it has anticancer activities including apoptosis and anti-angiogenesis. NCTD reduced VEGF production and the expression of integrin-β1 in tumor cells even at the low concentrations with less toxicity to the kidney or liver, an...Show More