Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (<i>Dioscorea species</i>) Via Nodal Culture

Berihu Mengs

doi:doi:10.11648/j.wjfst.20220601.12

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Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture

Berihu Mengs

Published in World Journal of Food Science and Technology (Volume 6, Issue 1)

Received: 25 March 2022 Accepted: 20 April 2022 Published: 28 April 2022

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Abstract

Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l indol-3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23±0.21) with a maximum shoot length 7.68±0.24cm and 6.79±0.09 shoot number with 7.47±0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03±0.49 and root length 10.76±0.16 cm and 10.58±0.26 root number with 10.42±0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.

Published in	World Journal of Food Science and Technology (Volume 6, Issue 1)
DOI	10.11648/j.wjfst.20220601.12
Page(s)	10-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Plant Hormones, Micro-Propagation, in vitro Nodal Culture, Dioscorea species and Yam

References

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Berihu Mengs. (2022). Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World Journal of Food Science and Technology, 6(1), 10-18. https://doi.org/10.11648/j.wjfst.20220601.12

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Berihu Mengs. Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World J. Food Sci. Technol. 2022, 6(1), 10-18. doi: 10.11648/j.wjfst.20220601.12

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Berihu Mengs. Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World J Food Sci Technol. 2022;6(1):10-18. doi: 10.11648/j.wjfst.20220601.12

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@article{10.11648/j.wjfst.20220601.12,
  author = {Berihu Mengs},
  title = {Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture},
  journal = {World Journal of Food Science and Technology},
  volume = {6},
  number = {1},
  pages = {10-18},
  doi = {10.11648/j.wjfst.20220601.12},
  url = {https://doi.org/10.11648/j.wjfst.20220601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20220601.12},
  abstract = {Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l indol-3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23±0.21) with a maximum shoot length 7.68±0.24cm and 6.79±0.09 shoot number with 7.47±0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03±0.49 and root length 10.76±0.16 cm and 10.58±0.26 root number with 10.42±0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.},
 year = {2022}
}

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TY  - JOUR
T1  - Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture
AU  - Berihu Mengs
Y1  - 2022/04/28
PY  - 2022
N1  - https://doi.org/10.11648/j.wjfst.20220601.12
DO  - 10.11648/j.wjfst.20220601.12
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 10
EP  - 18
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20220601.12
AB  - Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l indol-3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23±0.21) with a maximum shoot length 7.68±0.24cm and 6.79±0.09 shoot number with 7.47±0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03±0.49 and root length 10.76±0.16 cm and 10.58±0.26 root number with 10.42±0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.
VL  - 6
IS  - 1
ER  -

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Berihu Mengs

Plant Biotechnology Lab, Jimma Agricultural Research Center, Jimma, Ethiopia

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Berihu Mengs. (2022). Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World Journal of Food Science and Technology, 6(1), 10-18. https://doi.org/10.11648/j.wjfst.20220601.12

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Berihu Mengs. Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World J. Food Sci. Technol. 2022, 6(1), 10-18. doi: 10.11648/j.wjfst.20220601.12

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Berihu Mengs. Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture. World J Food Sci Technol. 2022;6(1):10-18. doi: 10.11648/j.wjfst.20220601.12

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@article{10.11648/j.wjfst.20220601.12,
  author = {Berihu Mengs},
  title = {Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture},
  journal = {World Journal of Food Science and Technology},
  volume = {6},
  number = {1},
  pages = {10-18},
  doi = {10.11648/j.wjfst.20220601.12},
  url = {https://doi.org/10.11648/j.wjfst.20220601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjfst.20220601.12},
  abstract = {Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l indol-3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23±0.21) with a maximum shoot length 7.68±0.24cm and 6.79±0.09 shoot number with 7.47±0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03±0.49 and root length 10.76±0.16 cm and 10.58±0.26 root number with 10.42±0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.},
 year = {2022}
}

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TY  - JOUR
T1  - Effect of Plant Hormones for Micro Propagation on Improved and Landrace Yam Varieties (Dioscorea species) Via Nodal Culture
AU  - Berihu Mengs
Y1  - 2022/04/28
PY  - 2022
N1  - https://doi.org/10.11648/j.wjfst.20220601.12
DO  - 10.11648/j.wjfst.20220601.12
T2  - World Journal of Food Science and Technology
JF  - World Journal of Food Science and Technology
JO  - World Journal of Food Science and Technology
SP  - 10
EP  - 18
PB  - Science Publishing Group
SN  - 2637-6024
UR  - https://doi.org/10.11648/j.wjfst.20220601.12
AB  - Yam (Dioscorea species) is a monocotyledonous tuber forming tropical vine which belongs to the order Liliflorae, family Dioscoreaceae, and genus Dioscorea. Conventional propagation of yam (Dioscorea spp.) is limited due to low propagation rates and highly infected by pests. However, In vitro clonal propagation is the best alternative to mass multiplication over conventional propagation. Moreover, shoot multiplication and rooting induction experiments were incubated on hormone free MS medium within 30 g/l sugar and 7 g/l agar. For shoot multiplication the initiated shoots of both landraces were cultured on MS media supplemented with 0, 1, 2, 3, 4 and 5 mg/l benzyl amino purine (BAP). For root induction, shoot lets were cultured on MS media supplemented with 0, 1, 2, 3, 4, 5 and 6 mg/l indol-3-butyric acid (IBA). Finally, for acclimatization, in vitro multiplied plantlets were transferred to greenhouse for hardening off. The results showed that the interaction effects of both landraces with BAP and IBA concentrations was significantly influenced in vitro yam shoot multiplication and rooting induction respectively. The maximum shoot number (7.23±0.21) with a maximum shoot length 7.68±0.24cm and 6.79±0.09 shoot number with 7.47±0.47 cm were obtained on MS media supplemented with 3 mg/l BAP for 11/02 and Koffea cultivars respectively. The maximum root number 10.03±0.49 and root length 10.76±0.16 cm and 10.58±0.26 root number with 10.42±0.32 cm were recorded on MS media with 3 mg/l IBA for Koffea and 11/02 cultivar respectively. In vitro raised plantlets were acclimatized and recorded 86% and 90% of survival rate in Koffea and 11/02, respectively, on soil medium with combination ratio of 2:1:1 top soil, sand soil and compost, respectively.
VL  - 6
IS  - 1
ER  -

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