Abstract: The study was conducted to evaluate physico-chemical properties of beeswax from Bale natural forest Southeastern of Ethiopia in 2014/15. For analysis fifteen (15) crude beeswax samples each of 1 to 3 kg were obtained from three locations considering the different agro-ecologies of Bale and compared against to the national and international standards. The physico-chemical characters evaluated were: melting point, specific gravity, refractive index, volatile matter, acid value, saponification value, ester value, and acid to ester ratio. Although there were some significant variation (P <0.05) between the different agro-ecologies, all samples collected were found in the range of the national and international standard limits. The means results of beeswax sample collected were Melting Point (62.39±0.12), Acid Value (22.33±0.39), Ester Value (75.58±0.84), Ester to Acid Ratio (3.38±0.07), Saponification Value (98.04±0.86), Specific gravity (0.9598±0.001), Refractive Index (1.4426±0.0002) and Volatile Matter (0.3335±0.027). Results obtained in this study, indicate that beeswax obtained from Bale show excellent quality characters according to the national and international standards. Hence, it is advisable to utilize the potentials for export market with better improved technology interventions.Abstract: The study was conducted to evaluate physico-chemical properties of beeswax from Bale natural forest Southeastern of Ethiopia in 2014/15. For analysis fifteen (15) crude beeswax samples each of 1 to 3 kg were obtained from three locations considering the different agro-ecologies of Bale and compared against to the national and international standard...Show More