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Otomycosis in Marrakech: Prospective Study of 19 Cases
El Mezouari El Mostafa,
Mouhib H.,
Darouassi Y.,
Ammar H.,
Moutaj R.
Issue:
Volume 2, Issue 1, March 2017
Pages:
1-3
Received:
25 November 2016
Accepted:
28 December 2016
Published:
23 January 2017
DOI:
10.11648/j.ijidt.20170201.11
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Abstract: Fungal otitis is an acute or subacute infection evolution, sometimes serious and rebellious to treatment. The objective of this work is to determine the prevalence of this infection in patients diagnosed at the laboratory of parasitology and mycology of the military hospital of Marrakech. A prospective study was performed that included patients with clinically suspected otomycosis. The ear removal is practiced during the otoscopic examination and diagnosis was confirmed by direct examination and culture. 19 cases of otomycosiswere diagnosed in 52 patients. Isolated fungal species were dominated by molds with a prevalence of 68.4% (N = 13) followed by the yeast with 31.6% (N = 6). It was not noted association of two fungal species. Aspergillusniger (35%), Aspergillusflavus (29%) and Candida albicans (13%) accounted for the most isolated fungal species. Otomycosis prevalence varies among studies. It is an infection that remains rebellious to treatment and whose management is difficult. It must be multi disciplinary combining mycological study as a diagnostic tool.
Abstract: Fungal otitis is an acute or subacute infection evolution, sometimes serious and rebellious to treatment. The objective of this work is to determine the prevalence of this infection in patients diagnosed at the laboratory of parasitology and mycology of the military hospital of Marrakech. A prospective study was performed that included patients wit...
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Survey of Sarcocystis Species Infection in Slaughtered Goats in Makurdi Metropolis
Faith Odije Okita,
Happiness Igwe Obadiah,
Kalemba Terlumun Gyegweh,
Adaobi Augustina Okonkwo,
John Aza Azatyom
Issue:
Volume 2, Issue 1, March 2017
Pages:
4-8
Received:
13 December 2016
Accepted:
4 January 2017
Published:
23 January 2017
DOI:
10.11648/j.ijidt.20170201.12
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Abstract: The emerging zoonotic infection caused by Sarcocystis species is becoming a serious public health problem for animal rearers .This study was conducted to determine the prevalence of Sarcocystis species infection in slaughtered goats at some selected abattoirs in Makurdi metropolis. Histological investigation was carried out using Haematoxylin and Eosin (H and E) as the staining agent on the tissue samples of heart (21), oesophagus (191) and tongue (183) from 224 goats. The results showed that, out of the 224 goats examined, 84(37.50%) were observed to be infected with Sarcocystis species. Morphologically some appeared round, ovoid or globular while others were elongated, spindle-shaped that tapered at one end. Infection related to sex showed that females had higher (83.33%) rate than males (16.67%).With respect to infection due to age, 9.52, 28.57 and 61.91% for ages 1-2 years, 2-3 years and above 3 years respectively were noted. Sex and age of the animals were not determining factors of the infection (P>0.05).There was significant difference in prevalence of infection in the three muscular tissues examined, with oesophagus having the highest (63.10 %) followed by the tongue (34.52%) and heart being the least (2.38%) (P< 0.05). The presence of two distinct microscopic cyst shapes (elongated, spindle-shaped that taper at one end and the round, oval or globular form) prompts the need for further research to ascertain species-specific associated with this parasite in goats and other animals in Nigeria.
Abstract: The emerging zoonotic infection caused by Sarcocystis species is becoming a serious public health problem for animal rearers .This study was conducted to determine the prevalence of Sarcocystis species infection in slaughtered goats at some selected abattoirs in Makurdi metropolis. Histological investigation was carried out using Haematoxylin and E...
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Serological Diagnostic Assays for Detection of Ns1 Antigen, IGM and IGG Antbodies to Dengue Virus
Purimitla Usha Rani,
Payala Vijayalakshmi
Issue:
Volume 2, Issue 1, March 2017
Pages:
9-14
Received:
18 December 2016
Accepted:
10 January 2017
Published:
15 February 2017
DOI:
10.11648/j.ijidt.20170201.13
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Abstract: Complications in managing the dengue virus infections include the lack of rapid diagnostic procedures and at the same time the symptoms of dengue infection are often confused with those of other diseases. Two commercial rapid serological diagnostic kit methods (Dengue Day 1 test, J Mitra and Co. Pvt. Ltd., New Delhi, ImmunoComb II Dengue IgM/IgGBispot kit (Orgenics Pvt. Ltd., Israel)were evaluated for the detection of NS1 antigen, Immunoglobulin IgG and IgM specific to dengue virus in the serum samples of patients suffered with dengue acute primary infection and secondary infection. The total assay time was 20 min-2hrs. The results of these methods were compared with the gold standard assay methods Dengue IgM-Capture Microplate ELISA and Dengue Indirect IgG ELISA (Pan Bio, Brisbane, Australia). The total assay time was 6-7hrs. Nine serum samples were positive to NS1 antigen and negative to IgG and IgM by Dengue day 1 test. The results of Bispot assay method revealed that, the number of IgG positive samples was 11, IgM positive samples were 31 and both IgG and IgM positive samples were 8. Majority of the positive cases were noticed in the age group 35-68 years and males were more prone to dengue infection while comparing with females. By performing IgM MICROLISA, 34 samples were positive which in turn indicated that, three of them were false negative by the immune comb bispot method giving a sensitivity of 91.17%. Through indirect IgG ELISA, the number of positive samples was 15 and four of the 15 positive samples of IgG were false negative by the immuo comb bispot method giving a sensitivity of 73.33%. The gold standard ELISA methods were more efficient than rapid serological tests and gave an overall sensitivity of 99%. Thus the alternative of an assay that is to be used in the diagnosis of dengue infections depends on factors like laboratory infrastructure, preference and availability of equipment. The allied performance of the Rapid test, followed by confirmation with MAC-ELISA on those samples, ensures both speediness as well as quality of reported results.
Abstract: Complications in managing the dengue virus infections include the lack of rapid diagnostic procedures and at the same time the symptoms of dengue infection are often confused with those of other diseases. Two commercial rapid serological diagnostic kit methods (Dengue Day 1 test, J Mitra and Co. Pvt. Ltd., New Delhi, ImmunoComb II Dengue IgM/IgGBis...
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Identification and Classification of Sand Flies Species and It’s Habitats in El-Kadaba Village, White Nile State, Sudan
Belal Abdallah Ahmed Adam,
Moawia Mukhtar Hassan,
Osman Mohammed Abdelnour,
Ahmed Hamid Awadallah
Issue:
Volume 2, Issue 1, March 2017
Pages:
15-21
Received:
28 December 2016
Accepted:
31 January 2017
Published:
24 February 2017
DOI:
10.11648/j.ijidt.20170201.14
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Abstract: Sand flies are a group of dipterans insects belonging to the subfamily Phlebotomine, family Psychodidae. Members of sand flies are delicate and can be easily recognized by their brownish colour, small size (1.5-2.5 mm), hairy appearance, long slender legs, jerky flight pattern and the characteristic manner in which they hold their pointed wings at an angle of 45° above their body. In this study, an entomological survey was carried out during June 2008 in El-Kadaba village (White Nile State, Sudan) to determine sandfly fauna and to identify sand flies vector(s). Sandflies were collected using sticky paper and light traps. Ten species of sandflies were recorded, three Phlebotomus species and seven Sergentomyia species of these sandflies, P. rodhaini was collected only from Acacia seyal/Balanitiesaegyptiaca at the Island whereas S. hunti was collected from the Acacia nilotica forest only of the total collections, P. orientalis representing 3.80% (248 specimens) of the total collection and P. papatasiwere 3.11% of the collection. The abundant species recorded in the area were S. sqaumipluer is (39.76%), S. clydei (25.89%) and S. antennatus (12.39%). Phlebotomusorientalis were collected significantly in higher numbers from the Island (Acacia/Balanities thicket) (5.50±0.98), than the village (3.25±0.72). According to results of this study we recommended the following: more studies must be done in future to cover all the belt of sand flies to determine all species of sand flies in Sudan. Annually Entomological survies must be done to determine density of Sandfly Vectors and Encourage the use of personal protection tools (ITNs), repellents, and improving of houses to avoid bite of sand fly.
Abstract: Sand flies are a group of dipterans insects belonging to the subfamily Phlebotomine, family Psychodidae. Members of sand flies are delicate and can be easily recognized by their brownish colour, small size (1.5-2.5 mm), hairy appearance, long slender legs, jerky flight pattern and the characteristic manner in which they hold their pointed wings at ...
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Early Diagnosis and Treatment Problems with Perinatal Tuberculosis as a Challenge to Health Care Providers
Eduardo Alfredo Duro,
Elizabeth Marcela Rizzo
Issue:
Volume 2, Issue 1, March 2017
Pages:
22-24
Received:
18 January 2017
Accepted:
6 February 2017
Published:
24 February 2017
DOI:
10.11648/j.ijidt.20170201.15
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Abstract: The diagnosis of tuberculosis (TB) in a newborn is often difficult as clinical signs are nonspecific. Neonatal infection is rare but it has a mortality up to 50%. The suspicion of TB in the mother is a powerful tool for diagnosis in the neonate, but physicians need to make mandatory steps to consider maternal TB in countries where the incidence is high. In this report we present two newborns with perinatal TB, the different clinical aspects in their mothers, and the problems that arised during investigation and diagnosis. We emphasize the need for improvement regarding screening of women at risk and sensitization of the medical community about this entity. We need to think in TB.
Abstract: The diagnosis of tuberculosis (TB) in a newborn is often difficult as clinical signs are nonspecific. Neonatal infection is rare but it has a mortality up to 50%. The suspicion of TB in the mother is a powerful tool for diagnosis in the neonate, but physicians need to make mandatory steps to consider maternal TB in countries where the incidence is ...
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